Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0361 and Psyr_0362 overlap by 4 nucleotides Psyr_0362 and Psyr_0363 are separated by 103 nucleotides Psyr_0363 and Psyr_0364 are separated by 105 nucleotides
Psyr_0361: Psyr_0361 - conserved hypothetical protein, at 386,578 to 387,285
_0361
Psyr_0362: Psyr_0362 - Sigma-70 region 2, at 387,282 to 387,848
_0362
Psyr_0363: Psyr_0363 - conserved hypothetical protein, at 387,952 to 388,485
_0363
Psyr_0364: Psyr_0364 - sodium/proton antiporter, NhaA family, at 388,591 to 389,766
_0364
Position (kb) 387
388
389 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 387.069 kb on + strand, within Psyr_0361 at 387.199 kb on + strand, within Psyr_0361 at 387.199 kb on + strand, within Psyr_0361 at 387.200 kb on - strand, within Psyr_0361 at 387.200 kb on - strand, within Psyr_0361 at 387.200 kb on - strand, within Psyr_0361 at 387.200 kb on - strand, within Psyr_0361 at 387.200 kb on - strand, within Psyr_0361 at 387.235 kb on + strand at 387.235 kb on + strand at 387.235 kb on + strand at 387.235 kb on + strand at 387.235 kb on + strand at 387.235 kb on + strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.236 kb on - strand at 387.239 kb on + strand at 387.239 kb on + strand at 387.239 kb on + strand at 387.239 kb on + strand at 387.239 kb on + strand at 387.240 kb on - strand at 387.240 kb on - strand at 387.240 kb on - strand at 387.272 kb on - strand at 387.272 kb on - strand at 387.272 kb on - strand at 387.390 kb on + strand, within Psyr_0362 at 387.390 kb on + strand, within Psyr_0362 at 387.390 kb on + strand, within Psyr_0362 at 387.391 kb on - strand, within Psyr_0362 at 387.391 kb on - strand, within Psyr_0362 at 387.391 kb on - strand, within Psyr_0362 at 387.391 kb on - strand, within Psyr_0362 at 387.391 kb on - strand, within Psyr_0362 at 387.394 kb on + strand, within Psyr_0362 at 387.394 kb on + strand, within Psyr_0362 at 387.394 kb on + strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.395 kb on - strand, within Psyr_0362 at 387.407 kb on + strand, within Psyr_0362 at 387.407 kb on + strand, within Psyr_0362 at 387.407 kb on + strand, within Psyr_0362 at 387.407 kb on + strand, within Psyr_0362 at 387.408 kb on - strand, within Psyr_0362 at 387.873 kb on + strand at 387.873 kb on + strand at 387.874 kb on - strand at 387.874 kb on - strand at 387.914 kb on - strand at 387.914 kb on - strand at 387.918 kb on + strand at 387.918 kb on + strand at 387.918 kb on + strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.919 kb on - strand at 387.940 kb on + strand at 387.975 kb on - strand at 387.986 kb on + strand at 387.986 kb on + strand at 387.987 kb on - strand at 388.073 kb on + strand, within Psyr_0363 at 388.073 kb on + strand, within Psyr_0363 at 388.074 kb on - strand, within Psyr_0363 at 388.074 kb on - strand, within Psyr_0363 at 388.074 kb on - strand, within Psyr_0363 at 388.074 kb on - strand, within Psyr_0363 at 388.074 kb on - strand, within Psyr_0363 at 388.130 kb on + strand, within Psyr_0363 at 388.210 kb on - strand, within Psyr_0363 at 388.223 kb on + strand, within Psyr_0363 at 388.224 kb on - strand, within Psyr_0363 at 388.226 kb on + strand, within Psyr_0363 at 388.226 kb on + strand, within Psyr_0363 at 388.226 kb on + strand, within Psyr_0363 at 388.226 kb on + strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.227 kb on - strand, within Psyr_0363 at 388.328 kb on + strand, within Psyr_0363 at 388.328 kb on + strand, within Psyr_0363 at 388.328 kb on + strand, within Psyr_0363 at 388.329 kb on - strand, within Psyr_0363 at 388.329 kb on - strand, within Psyr_0363 at 388.329 kb on - strand, within Psyr_0363 at 388.329 kb on - strand, within Psyr_0363 at 388.329 kb on - strand, within Psyr_0363 at 388.453 kb on - strand at 388.542 kb on + strand at 388.542 kb on + strand at 388.542 kb on + strand at 388.542 kb on + strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.543 kb on - strand at 388.545 kb on + strand at 388.585 kb on - strand at 388.661 kb on - strand at 388.766 kb on + strand, within Psyr_0364 at 388.914 kb on + strand, within Psyr_0364 at 388.915 kb on - strand, within Psyr_0364 at 388.915 kb on - strand, within Psyr_0364 at 388.915 kb on - strand, within Psyr_0364 at 388.982 kb on + strand, within Psyr_0364 at 388.983 kb on - strand, within Psyr_0364 at 388.983 kb on - strand, within Psyr_0364 at 389.136 kb on - strand, within Psyr_0364 at 389.136 kb on - strand, within Psyr_0364 at 389.136 kb on - strand, within Psyr_0364 at 389.234 kb on + strand, within Psyr_0364 at 389.234 kb on + strand, within Psyr_0364 at 389.234 kb on + strand, within Psyr_0364 at 389.235 kb on - strand, within Psyr_0364 at 389.423 kb on + strand, within Psyr_0364 at 389.423 kb on + strand, within Psyr_0364 at 389.423 kb on + strand, within Psyr_0364 at 389.423 kb on + strand, within Psyr_0364 at 389.424 kb on - strand, within Psyr_0364
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 387,069 + Psyr_0361 0.69 +0.4 387,199 + Psyr_0361 0.88 -3.0 387,199 + Psyr_0361 0.88 +0.8 387,200 - Psyr_0361 0.88 -1.3 387,200 - Psyr_0361 0.88 +0.9 387,200 - Psyr_0361 0.88 -0.0 387,200 - Psyr_0361 0.88 -0.9 387,200 - Psyr_0361 0.88 -1.4 387,235 + -2.0 387,235 + -0.0 387,235 + +1.2 387,235 + -1.7 387,235 + -0.9 387,235 + -2.0 387,236 - +0.1 387,236 - +1.3 387,236 - +1.6 387,236 - +2.1 387,236 - -4.3 387,236 - -0.4 387,236 - -0.1 387,239 + +1.1 387,239 + +1.2 387,239 + -2.9 387,239 + +0.1 387,239 + -3.3 387,240 - +0.5 387,240 - +2.0 387,240 - -3.6 387,272 - +1.4 387,272 - +1.2 387,272 - +0.0 387,390 + Psyr_0362 0.19 -0.9 387,390 + Psyr_0362 0.19 -3.8 387,390 + Psyr_0362 0.19 -2.0 387,391 - Psyr_0362 0.19 -0.2 387,391 - Psyr_0362 0.19 -3.1 387,391 - Psyr_0362 0.19 +0.3 387,391 - Psyr_0362 0.19 +2.9 387,391 - Psyr_0362 0.19 -1.3 387,394 + Psyr_0362 0.20 +1.8 387,394 + Psyr_0362 0.20 -0.0 387,394 + Psyr_0362 0.20 -3.2 387,395 - Psyr_0362 0.20 -1.5 387,395 - Psyr_0362 0.20 -1.9 387,395 - Psyr_0362 0.20 +0.0 387,395 - Psyr_0362 0.20 -1.7 387,395 - Psyr_0362 0.20 -2.5 387,395 - Psyr_0362 0.20 -0.1 387,407 + Psyr_0362 0.22 +1.1 387,407 + Psyr_0362 0.22 -2.3 387,407 + Psyr_0362 0.22 -2.2 387,407 + Psyr_0362 0.22 +0.3 387,408 - Psyr_0362 0.22 +1.4 387,873 + +0.4 387,873 + +1.2 387,874 - -0.6 387,874 - -0.9 387,914 - -0.6 387,914 - -3.2 387,918 + -3.3 387,918 + -0.0 387,918 + +2.1 387,919 - -2.4 387,919 - +1.2 387,919 - +2.2 387,919 - +2.2 387,919 - -0.2 387,919 - +0.6 387,919 - -0.8 387,940 + -0.5 387,975 - +1.0 387,986 + -3.6 387,986 + +0.2 387,987 - +0.6 388,073 + Psyr_0363 0.23 -1.9 388,073 + Psyr_0363 0.23 -2.0 388,074 - Psyr_0363 0.23 +0.1 388,074 - Psyr_0363 0.23 -2.8 388,074 - Psyr_0363 0.23 -0.8 388,074 - Psyr_0363 0.23 +0.5 388,074 - Psyr_0363 0.23 -0.4 388,130 + Psyr_0363 0.33 -3.0 388,210 - Psyr_0363 0.48 +0.1 388,223 + Psyr_0363 0.51 -0.5 388,224 - Psyr_0363 0.51 -2.3 388,226 + Psyr_0363 0.51 +1.3 388,226 + Psyr_0363 0.51 +0.5 388,226 + Psyr_0363 0.51 -0.3 388,226 + Psyr_0363 0.51 -1.8 388,227 - Psyr_0363 0.51 -0.1 388,227 - Psyr_0363 0.51 +1.8 388,227 - Psyr_0363 0.51 -1.2 388,227 - Psyr_0363 0.51 +1.1 388,227 - Psyr_0363 0.51 -0.7 388,227 - Psyr_0363 0.51 -0.3 388,328 + Psyr_0363 0.70 -2.0 388,328 + Psyr_0363 0.70 -1.6 388,328 + Psyr_0363 0.70 -1.8 388,329 - Psyr_0363 0.71 -1.3 388,329 - Psyr_0363 0.71 +0.5 388,329 - Psyr_0363 0.71 +2.4 388,329 - Psyr_0363 0.71 -3.2 388,329 - Psyr_0363 0.71 +1.4 388,453 - +0.7 388,542 + -0.2 388,542 + +0.5 388,542 + -1.8 388,542 + -0.1 388,543 - -0.8 388,543 - -1.3 388,543 - +1.8 388,543 - -0.6 388,543 - -1.8 388,543 - +1.4 388,543 - -0.2 388,543 - +1.3 388,545 + +1.5 388,585 - +0.7 388,661 - +0.5 388,766 + Psyr_0364 0.15 -1.3 388,914 + Psyr_0364 0.27 -0.4 388,915 - Psyr_0364 0.28 -2.9 388,915 - Psyr_0364 0.28 +0.8 388,915 - Psyr_0364 0.28 -3.8 388,982 + Psyr_0364 0.33 -2.7 388,983 - Psyr_0364 0.33 -1.9 388,983 - Psyr_0364 0.33 -2.9 389,136 - Psyr_0364 0.46 -1.0 389,136 - Psyr_0364 0.46 -4.0 389,136 - Psyr_0364 0.46 -2.1 389,234 + Psyr_0364 0.55 -0.1 389,234 + Psyr_0364 0.55 -0.4 389,234 + Psyr_0364 0.55 -0.6 389,235 - Psyr_0364 0.55 -2.5 389,423 + Psyr_0364 0.71 +1.3 389,423 + Psyr_0364 0.71 +1.8 389,423 + Psyr_0364 0.71 -0.0 389,423 + Psyr_0364 0.71 +2.4 389,424 - Psyr_0364 0.71 -2.7
Or see this region's nucleotide sequence