Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0291 and Psyr_0292 are separated by 231 nucleotides Psyr_0292 and Psyr_0293 are separated by 16 nucleotides
Psyr_0291: Psyr_0291 - DedA, at 309,519 to 310,181
_0291
Psyr_0292: Psyr_0292 - porphobilinogen synthase, at 310,413 to 311,423
_0292
Psyr_0293: Psyr_0293 - Polyphosphate kinase, at 311,440 to 313,650
_0293
Position (kb) 310
311
312 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 309.521 kb on - strand at 309.709 kb on + strand, within Psyr_0291 at 309.710 kb on - strand, within Psyr_0291 at 309.758 kb on - strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 309.817 kb on + strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.007 kb on - strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.027 kb on + strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.028 kb on - strand, within Psyr_0291 at 310.044 kb on + strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.073 kb on - strand, within Psyr_0291 at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.246 kb on + strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.247 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.289 kb on - strand at 310.367 kb on + strand at 310.367 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 310.369 kb on + strand at 311.445 kb on + strand at 311.522 kb on + strand at 311.523 kb on - strand at 311.523 kb on - strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.596 kb on + strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.597 kb on - strand at 311.963 kb on - strand, within Psyr_0293 at 312.251 kb on - strand, within Psyr_0293 at 312.362 kb on + strand, within Psyr_0293 at 312.363 kb on - strand, within Psyr_0293
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 309,521 - -1.5 309,709 + Psyr_0291 0.29 -2.4 309,710 - Psyr_0291 0.29 -1.8 309,758 - Psyr_0291 0.36 -0.3 309,817 + Psyr_0291 0.45 -1.8 309,817 + Psyr_0291 0.45 -2.7 310,007 - Psyr_0291 0.74 -3.8 310,007 - Psyr_0291 0.74 -2.8 310,007 - Psyr_0291 0.74 +1.1 310,027 + Psyr_0291 0.77 -3.2 310,027 + Psyr_0291 0.77 -2.0 310,027 + Psyr_0291 0.77 +0.9 310,028 - Psyr_0291 0.77 -2.7 310,028 - Psyr_0291 0.77 -2.8 310,044 + Psyr_0291 0.79 -2.0 310,073 - Psyr_0291 0.84 -1.2 310,073 - Psyr_0291 0.84 -3.7 310,246 + -0.1 310,246 + -2.7 310,246 + +2.1 310,246 + -1.0 310,246 + -0.2 310,246 + -3.4 310,246 + -0.1 310,246 + -1.2 310,246 + -3.4 310,246 + -1.1 310,246 + -2.4 310,246 + -1.0 310,246 + +0.3 310,246 + +0.1 310,247 - -3.2 310,247 - -3.2 310,247 - +0.1 310,247 - +2.2 310,247 - +1.1 310,247 - +2.5 310,247 - -1.9 310,247 - +0.5 310,247 - +0.8 310,247 - -2.0 310,247 - -2.4 310,247 - +0.1 310,247 - +0.3 310,289 - +0.9 310,289 - +0.1 310,289 - -2.3 310,367 + +0.3 310,367 + +2.0 310,369 + +4.0 310,369 + -0.3 310,369 + +0.8 310,369 + -0.9 310,369 + -0.9 310,369 + -1.1 310,369 + -2.5 311,445 + +0.2 311,522 + +3.4 311,523 - -3.0 311,523 - +0.0 311,596 + -4.4 311,596 + +0.7 311,596 + +1.7 311,596 + -3.0 311,596 + -3.7 311,596 + -1.8 311,596 + -0.4 311,596 + +0.1 311,597 - -0.1 311,597 - -3.9 311,597 - -0.3 311,597 - -2.1 311,597 - -1.2 311,597 - -3.5 311,597 - +0.2 311,597 - -0.2 311,597 - -4.6 311,597 - -1.0 311,597 - +0.5 311,597 - +1.3 311,597 - -2.8 311,963 - Psyr_0293 0.24 -3.5 312,251 - Psyr_0293 0.37 -0.2 312,362 + Psyr_0293 0.42 -2.0 312,363 - Psyr_0293 0.42 +1.0
Or see this region's nucleotide sequence