Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0262 and Psyr_0263 are separated by 197 nucleotides Psyr_0263 and Psyr_0264 are separated by 10 nucleotides
Psyr_0262: Psyr_0262 - Transport-associated protein, at 277,811 to 278,650
_0262
Psyr_0263: Psyr_0263 - Two-component response regulator AlgB, at 278,848 to 280,194
_0263
Psyr_0264: Psyr_0264 - Alginate biosynthesis sensor protein KinB, at 280,205 to 281,992
_0264
Position (kb) 278
279
280
281 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 277.874 kb on - strand at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.992 kb on + strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 277.993 kb on - strand, within Psyr_0262 at 278.650 kb on + strand at 278.651 kb on - strand at 278.706 kb on + strand at 278.706 kb on + strand at 278.736 kb on + strand at 278.737 kb on - strand at 278.753 kb on - strand at 278.753 kb on - strand at 278.814 kb on + strand at 278.814 kb on + strand at 278.840 kb on + strand at 278.944 kb on + strand at 278.945 kb on - strand at 278.945 kb on - strand at 278.945 kb on - strand at 279.002 kb on + strand, within Psyr_0263 at 279.003 kb on - strand, within Psyr_0263 at 279.163 kb on + strand, within Psyr_0263 at 279.164 kb on - strand, within Psyr_0263 at 279.650 kb on - strand, within Psyr_0263 at 279.763 kb on + strand, within Psyr_0263 at 279.764 kb on - strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.865 kb on + strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.866 kb on - strand, within Psyr_0263 at 279.919 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.995 kb on + strand, within Psyr_0263 at 279.996 kb on - strand, within Psyr_0263 at 280.100 kb on + strand at 280.100 kb on + strand at 280.101 kb on - strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.183 kb on + strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.184 kb on - strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.190 kb on + strand at 280.191 kb on - strand at 280.191 kb on - strand at 280.197 kb on + strand at 280.197 kb on + strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.198 kb on - strand at 280.319 kb on - strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.342 kb on + strand at 280.538 kb on + strand, within Psyr_0264 at 280.539 kb on - strand, within Psyr_0264 at 280.568 kb on + strand, within Psyr_0264 at 280.569 kb on - strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.590 kb on + strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 280.591 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264 at 281.003 kb on - strand, within Psyr_0264
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 277,874 - -2.5 277,992 + Psyr_0262 0.22 +1.1 277,992 + Psyr_0262 0.22 +2.6 277,992 + Psyr_0262 0.22 +1.2 277,992 + Psyr_0262 0.22 -0.4 277,992 + Psyr_0262 0.22 -2.6 277,992 + Psyr_0262 0.22 +1.1 277,992 + Psyr_0262 0.22 -0.4 277,993 - Psyr_0262 0.22 -1.5 277,993 - Psyr_0262 0.22 -1.3 277,993 - Psyr_0262 0.22 +2.4 277,993 - Psyr_0262 0.22 -3.7 277,993 - Psyr_0262 0.22 -3.1 278,650 + +0.2 278,651 - +0.9 278,706 + +0.8 278,706 + +1.5 278,736 + +0.2 278,737 - -0.6 278,753 - +3.0 278,753 - -1.1 278,814 + +0.8 278,814 + -3.3 278,840 + -1.8 278,944 + -3.4 278,945 - +1.5 278,945 - +1.6 278,945 - +2.1 279,002 + Psyr_0263 0.11 -1.2 279,003 - Psyr_0263 0.12 -1.7 279,163 + Psyr_0263 0.23 +2.1 279,164 - Psyr_0263 0.23 -0.7 279,650 - Psyr_0263 0.60 +3.3 279,763 + Psyr_0263 0.68 +1.4 279,764 - Psyr_0263 0.68 +2.1 279,865 + Psyr_0263 0.76 +0.6 279,865 + Psyr_0263 0.76 -2.5 279,865 + Psyr_0263 0.76 -1.5 279,866 - Psyr_0263 0.76 -0.9 279,866 - Psyr_0263 0.76 +1.0 279,919 + Psyr_0263 0.80 +3.0 279,995 + Psyr_0263 0.85 +2.4 279,995 + Psyr_0263 0.85 +0.6 279,996 - Psyr_0263 0.85 +2.1 280,100 + +0.1 280,100 + +1.9 280,101 - -0.1 280,183 + +3.4 280,183 + +0.9 280,183 + +0.6 280,184 - +1.9 280,184 - +1.0 280,184 - +0.8 280,190 + +1.0 280,190 + +0.5 280,190 + -1.7 280,190 + -0.3 280,191 - +1.7 280,191 - +1.7 280,197 + -2.5 280,197 + +0.8 280,198 - +2.6 280,198 - -1.0 280,198 - +0.9 280,198 - -0.3 280,198 - +1.3 280,319 - -0.0 280,342 + -2.1 280,342 + -2.2 280,342 + -1.1 280,342 + +5.8 280,538 + Psyr_0264 0.19 -0.3 280,539 - Psyr_0264 0.19 -3.4 280,568 + Psyr_0264 0.20 +0.6 280,569 - Psyr_0264 0.20 +1.3 280,590 + Psyr_0264 0.22 -0.3 280,590 + Psyr_0264 0.22 -2.0 280,591 - Psyr_0264 0.22 -0.2 280,591 - Psyr_0264 0.22 -0.8 280,591 - Psyr_0264 0.22 +0.1 281,003 - Psyr_0264 0.45 -1.2 281,003 - Psyr_0264 0.45 -0.9
Or see this region's nucleotide sequence