Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0018 and Psyr_0019 are separated by 53 nucleotides Psyr_0019 and Psyr_0020 are separated by 125 nucleotides Psyr_0020 and Psyr_0021 are separated by 123 nucleotides
Psyr_0018: Psyr_0018 - methionyl-tRNA formyltransferase, at 22,042 to 22,986
_0018
Psyr_0019: Psyr_0019 - peptide deformylase, at 23,040 to 23,546
_0019
Psyr_0020: Psyr_0020 - Peptidoglycan-binding LysM, at 23,672 to 24,697
_0020
Psyr_0021: Psyr_0021 - DNA protecting protein DprA, at 24,821 to 25,939
_0021
Position (kb) 23
24
25 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 22.999 kb on - strand at 22.999 kb on - strand at 23.536 kb on - strand at 23.547 kb on - strand at 23.547 kb on - strand at 23.547 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.549 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.553 kb on - strand at 23.633 kb on - strand at 23.633 kb on - strand at 23.657 kb on - strand at 23.685 kb on + strand at 23.686 kb on - strand at 23.686 kb on - strand at 23.762 kb on + strand at 23.762 kb on + strand at 23.762 kb on + strand at 23.762 kb on + strand at 23.762 kb on + strand at 23.762 kb on + strand at 23.763 kb on - strand at 23.763 kb on - strand at 23.987 kb on - strand, within Psyr_0020 at 24.092 kb on + strand, within Psyr_0020 at 24.092 kb on + strand, within Psyr_0020 at 24.093 kb on - strand, within Psyr_0020 at 24.093 kb on - strand, within Psyr_0020 at 24.093 kb on - strand, within Psyr_0020 at 24.093 kb on - strand, within Psyr_0020 at 24.093 kb on - strand, within Psyr_0020 at 24.146 kb on + strand, within Psyr_0020 at 24.147 kb on - strand, within Psyr_0020 at 24.147 kb on - strand, within Psyr_0020 at 24.182 kb on + strand, within Psyr_0020 at 24.183 kb on - strand, within Psyr_0020 at 24.183 kb on - strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.209 kb on + strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.210 kb on - strand, within Psyr_0020 at 24.349 kb on - strand, within Psyr_0020 at 24.349 kb on - strand, within Psyr_0020 at 24.371 kb on - strand, within Psyr_0020 at 24.527 kb on + strand, within Psyr_0020 at 24.527 kb on + strand, within Psyr_0020 at 24.527 kb on + strand, within Psyr_0020 at 24.527 kb on + strand, within Psyr_0020 at 24.528 kb on - strand, within Psyr_0020 at 24.528 kb on - strand, within Psyr_0020 at 24.620 kb on + strand at 24.620 kb on + strand at 24.620 kb on + strand at 24.621 kb on - strand at 24.621 kb on - strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.631 kb on + strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.632 kb on - strand at 24.635 kb on + strand at 24.745 kb on + strand at 24.745 kb on + strand at 24.745 kb on + strand at 24.745 kb on + strand at 24.746 kb on - strand at 24.769 kb on - strand at 24.897 kb on + strand at 25.348 kb on + strand, within Psyr_0021 at 25.348 kb on + strand, within Psyr_0021 at 25.349 kb on - strand, within Psyr_0021 at 25.349 kb on - strand, within Psyr_0021 at 25.349 kb on - strand, within Psyr_0021 at 25.349 kb on - strand, within Psyr_0021 at 25.527 kb on + strand, within Psyr_0021 at 25.528 kb on - strand, within Psyr_0021 at 25.528 kb on - strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.604 kb on + strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.605 kb on - strand, within Psyr_0021 at 25.612 kb on - strand, within Psyr_0021 at 25.659 kb on + strand, within Psyr_0021 at 25.660 kb on - strand, within Psyr_0021 at 25.660 kb on - strand, within Psyr_0021
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 22,999 - +1.4 22,999 - +0.2 23,536 - +0.6 23,547 - -1.9 23,547 - -0.2 23,547 - +2.5 23,549 - -0.0 23,549 - -2.0 23,549 - +0.2 23,549 - -0.7 23,549 - -3.1 23,549 - -1.8 23,549 - -2.1 23,549 - -2.8 23,549 - +1.6 23,549 - +0.9 23,549 - +0.3 23,549 - -2.2 23,549 - -2.9 23,549 - -1.4 23,549 - -2.7 23,549 - -3.8 23,549 - +3.0 23,549 - +1.2 23,549 - +2.7 23,549 - +1.6 23,549 - -2.2 23,549 - -1.6 23,549 - -0.6 23,549 - +0.3 23,549 - +0.6 23,549 - -1.0 23,549 - -3.4 23,549 - +4.3 23,549 - -2.3 23,549 - -0.9 23,549 - +3.7 23,553 - +0.5 23,553 - -0.7 23,553 - +1.7 23,553 - -2.2 23,553 - -3.3 23,553 - +0.6 23,553 - -0.8 23,553 - -2.8 23,553 - +0.4 23,553 - -3.1 23,633 - +1.1 23,633 - -2.9 23,657 - +0.5 23,685 + -2.9 23,686 - +2.2 23,686 - -0.6 23,762 + +1.1 23,762 + -2.8 23,762 + -4.0 23,762 + +2.0 23,762 + +0.8 23,762 + -2.8 23,763 - +1.6 23,763 - +1.2 23,987 - Psyr_0020 0.31 -2.8 24,092 + Psyr_0020 0.41 -2.6 24,092 + Psyr_0020 0.41 -1.7 24,093 - Psyr_0020 0.41 +0.1 24,093 - Psyr_0020 0.41 +1.7 24,093 - Psyr_0020 0.41 +0.6 24,093 - Psyr_0020 0.41 +2.0 24,093 - Psyr_0020 0.41 +1.3 24,146 + Psyr_0020 0.46 +3.8 24,147 - Psyr_0020 0.46 +0.4 24,147 - Psyr_0020 0.46 -1.6 24,182 + Psyr_0020 0.50 +1.1 24,183 - Psyr_0020 0.50 -2.7 24,183 - Psyr_0020 0.50 +0.9 24,209 + Psyr_0020 0.52 +0.3 24,209 + Psyr_0020 0.52 +4.7 24,209 + Psyr_0020 0.52 +0.3 24,209 + Psyr_0020 0.52 +1.6 24,209 + Psyr_0020 0.52 +0.2 24,209 + Psyr_0020 0.52 +0.7 24,209 + Psyr_0020 0.52 -1.3 24,210 - Psyr_0020 0.52 +0.7 24,210 - Psyr_0020 0.52 -0.2 24,210 - Psyr_0020 0.52 +0.1 24,210 - Psyr_0020 0.52 -1.7 24,210 - Psyr_0020 0.52 -1.1 24,210 - Psyr_0020 0.52 -0.0 24,210 - Psyr_0020 0.52 -0.5 24,349 - Psyr_0020 0.66 -2.1 24,349 - Psyr_0020 0.66 +2.3 24,371 - Psyr_0020 0.68 +1.5 24,527 + Psyr_0020 0.83 -2.1 24,527 + Psyr_0020 0.83 +0.7 24,527 + Psyr_0020 0.83 -2.9 24,527 + Psyr_0020 0.83 -0.3 24,528 - Psyr_0020 0.83 -1.4 24,528 - Psyr_0020 0.83 +4.6 24,620 + +0.6 24,620 + -1.3 24,620 + +0.8 24,621 - -3.7 24,621 - -1.5 24,631 + -2.2 24,631 + -0.9 24,631 + +1.7 24,631 + -1.6 24,631 + -0.7 24,631 + +1.1 24,631 + +0.6 24,631 + -0.9 24,631 + -2.8 24,631 + -0.9 24,631 + +1.1 24,631 + +0.0 24,632 - -3.6 24,632 - -2.7 24,632 - +1.6 24,632 - -2.7 24,632 - +0.5 24,632 - -2.7 24,632 - -1.8 24,632 - +1.9 24,632 - -1.9 24,632 - +1.0 24,632 - -0.1 24,635 + -2.0 24,745 + +1.5 24,745 + -2.7 24,745 + -0.5 24,745 + -0.7 24,746 - +0.9 24,769 - -3.3 24,897 + +1.2 25,348 + Psyr_0021 0.47 +1.6 25,348 + Psyr_0021 0.47 +0.7 25,349 - Psyr_0021 0.47 +3.5 25,349 - Psyr_0021 0.47 -3.0 25,349 - Psyr_0021 0.47 -1.5 25,349 - Psyr_0021 0.47 -0.9 25,527 + Psyr_0021 0.63 -2.4 25,528 - Psyr_0021 0.63 +0.0 25,528 - Psyr_0021 0.63 +1.1 25,604 + Psyr_0021 0.70 -0.1 25,604 + Psyr_0021 0.70 -0.4 25,604 + Psyr_0021 0.70 -0.7 25,604 + Psyr_0021 0.70 +1.1 25,604 + Psyr_0021 0.70 +0.4 25,604 + Psyr_0021 0.70 +2.2 25,604 + Psyr_0021 0.70 +0.4 25,605 - Psyr_0021 0.70 +2.2 25,605 - Psyr_0021 0.70 -1.2 25,605 - Psyr_0021 0.70 -1.2 25,605 - Psyr_0021 0.70 +0.6 25,605 - Psyr_0021 0.70 -3.4 25,605 - Psyr_0021 0.70 -1.5 25,605 - Psyr_0021 0.70 +0.2 25,612 - Psyr_0021 0.71 +1.8 25,659 + Psyr_0021 0.75 +1.5 25,660 - Psyr_0021 0.75 +0.4 25,660 - Psyr_0021 0.75 +1.6
Or see this region's nucleotide sequence