Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19925 and BWI76_RS19930 are separated by 185 nucleotides BWI76_RS19930 and BWI76_RS19935 are separated by 43 nucleotides BWI76_RS19935 and BWI76_RS19940 are separated by 112 nucleotides
BWI76_RS19925: BWI76_RS19925 - DNA gyrase subunit A, at 4,171,294 to 4,173,927
_RS19925
BWI76_RS19930: BWI76_RS19930 - bifunctional 3-demethylubiquinone 3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol methylase, at 4,174,113 to 4,174,841
_RS19930
BWI76_RS19935: BWI76_RS19935 - hypothetical protein, at 4,174,885 to 4,175,073
_RS19935
BWI76_RS19940: BWI76_RS19940 - ribonucleoside-diphosphate reductase subunit alpha, at 4,175,186 to 4,177,471
_RS19940
Position (kb)
4174
4175
4176 Strain fitness (log2 ratio)
-1
0
1 at 4173.981 kb on + strand at 4174.048 kb on - strand at 4174.814 kb on + strand at 4174.881 kb on - strand at 4174.881 kb on - strand at 4174.959 kb on + strand, within BWI76_RS19935
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 4,173,981 + +0.4 4,174,048 - +0.1 4,174,814 + -0.3 4,174,881 - +0.2 4,174,881 - +0.2 4,174,959 + BWI76_RS19935 0.39 -1.2
Or see this region's nucleotide sequence