Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotides BWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotides BWI76_RS19150 and BWI76_RS19155 are separated by 12 nucleotides
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633
_RS19145
BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532
_RS19150
BWI76_RS19155: BWI76_RS19155 - formate dehydrogenase-N subunit alpha, at 3,982,545 to 3,985,595
_RS19155
Position (kb)
3981
3982
3983 Strain fitness (log2 ratio)
-1
0
1 at 3980.662 kb on + strand at 3981.070 kb on - strand, within BWI76_RS19145 at 3982.721 kb on + strand at 3982.783 kb on - strand at 3982.820 kb on - strand at 3982.962 kb on + strand, within BWI76_RS19155 at 3983.019 kb on - strand, within BWI76_RS19155 at 3983.094 kb on + strand, within BWI76_RS19155 at 3983.174 kb on - strand, within BWI76_RS19155 at 3983.307 kb on + strand, within BWI76_RS19155 at 3983.410 kb on + strand, within BWI76_RS19155 at 3983.489 kb on + strand, within BWI76_RS19155 at 3983.497 kb on - strand, within BWI76_RS19155
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,980,662 + -1.0 3,981,070 - BWI76_RS19145 0.10 -0.0 3,982,721 + +0.1 3,982,783 - -0.1 3,982,820 - +0.2 3,982,962 + BWI76_RS19155 0.14 -0.7 3,983,019 - BWI76_RS19155 0.16 -0.3 3,983,094 + BWI76_RS19155 0.18 +0.2 3,983,174 - BWI76_RS19155 0.21 -0.2 3,983,307 + BWI76_RS19155 0.25 -1.2 3,983,410 + BWI76_RS19155 0.28 -0.1 3,983,489 + BWI76_RS19155 0.31 -0.5 3,983,497 - BWI76_RS19155 0.31 +0.7
Or see this region's nucleotide sequence