Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10025 and BWI76_RS10030 are separated by 235 nucleotides BWI76_RS10030 and BWI76_RS10035 are separated by 42 nucleotides
BWI76_RS10025: BWI76_RS10025 - secretion protein HlyD, at 2,122,349 to 2,123,554
_RS10025
BWI76_RS10030: BWI76_RS10030 - glycosyl transferase, at 2,123,790 to 2,126,933
_RS10030
BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908
_RS10035
Position (kb)
2123
2124
2125
2126
2127 Strain fitness (log2 ratio)
-1
0
1
2 at 2122.888 kb on - strand, within BWI76_RS10025 at 2122.926 kb on + strand, within BWI76_RS10025 at 2122.934 kb on - strand, within BWI76_RS10025 at 2122.941 kb on + strand, within BWI76_RS10025 at 2122.949 kb on - strand, within BWI76_RS10025 at 2123.018 kb on - strand, within BWI76_RS10025 at 2123.023 kb on + strand, within BWI76_RS10025 at 2123.046 kb on - strand, within BWI76_RS10025 at 2123.052 kb on - strand, within BWI76_RS10025 at 2123.057 kb on - strand, within BWI76_RS10025 at 2123.065 kb on - strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.090 kb on + strand, within BWI76_RS10025 at 2123.097 kb on - strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.111 kb on + strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.119 kb on - strand, within BWI76_RS10025 at 2123.137 kb on + strand, within BWI76_RS10025 at 2123.154 kb on + strand, within BWI76_RS10025 at 2123.164 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.169 kb on + strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.177 kb on - strand, within BWI76_RS10025 at 2123.214 kb on - strand, within BWI76_RS10025 at 2123.256 kb on - strand, within BWI76_RS10025 at 2123.324 kb on + strand, within BWI76_RS10025 at 2123.339 kb on - strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.348 kb on + strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.356 kb on - strand, within BWI76_RS10025 at 2123.392 kb on + strand, within BWI76_RS10025 at 2123.410 kb on - strand, within BWI76_RS10025 at 2123.456 kb on - strand at 2123.473 kb on + strand at 2123.527 kb on + strand at 2123.535 kb on - strand at 2123.565 kb on + strand at 2123.570 kb on + strand at 2123.570 kb on + strand at 2123.573 kb on - strand at 2123.578 kb on - strand at 2123.578 kb on - strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.610 kb on + strand at 2123.613 kb on - strand at 2123.615 kb on + strand at 2123.615 kb on + strand at 2123.618 kb on - strand at 2123.623 kb on - strand at 2123.623 kb on - strand at 2123.665 kb on - strand at 2123.681 kb on - strand at 2123.684 kb on + strand at 2123.692 kb on - strand at 2123.694 kb on - strand at 2123.696 kb on + strand at 2123.704 kb on - strand at 2123.763 kb on - strand at 2123.789 kb on - strand at 2123.791 kb on - strand at 2123.803 kb on + strand at 2123.863 kb on - strand at 2123.864 kb on - strand at 2123.864 kb on - strand at 2123.880 kb on + strand at 2123.966 kb on + strand at 2124.176 kb on + strand, within BWI76_RS10030 at 2124.184 kb on - strand, within BWI76_RS10030 at 2124.367 kb on - strand, within BWI76_RS10030 at 2124.537 kb on - strand, within BWI76_RS10030 at 2124.562 kb on + strand, within BWI76_RS10030 at 2124.936 kb on - strand, within BWI76_RS10030 at 2124.955 kb on + strand, within BWI76_RS10030 at 2125.278 kb on + strand, within BWI76_RS10030 at 2125.534 kb on + strand, within BWI76_RS10030 at 2125.541 kb on + strand, within BWI76_RS10030 at 2125.714 kb on + strand, within BWI76_RS10030 at 2125.759 kb on + strand, within BWI76_RS10030 at 2125.824 kb on - strand, within BWI76_RS10030 at 2125.943 kb on + strand, within BWI76_RS10030 at 2126.146 kb on - strand, within BWI76_RS10030 at 2126.443 kb on + strand, within BWI76_RS10030 at 2126.451 kb on - strand, within BWI76_RS10030 at 2126.451 kb on - strand at 2126.503 kb on - strand, within BWI76_RS10030 at 2126.770 kb on + strand at 2126.859 kb on + strand at 2126.880 kb on + strand at 2126.888 kb on - strand at 2127.018 kb on - strand at 2127.267 kb on + strand, within BWI76_RS10035 at 2127.326 kb on + strand, within BWI76_RS10035 at 2127.835 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 2,122,888 - BWI76_RS10025 0.45 +1.5 2,122,926 + BWI76_RS10025 0.48 +2.2 2,122,934 - BWI76_RS10025 0.49 +1.8 2,122,941 + BWI76_RS10025 0.49 +1.9 2,122,949 - BWI76_RS10025 0.50 +1.8 2,123,018 - BWI76_RS10025 0.55 +1.9 2,123,023 + BWI76_RS10025 0.56 +2.0 2,123,046 - BWI76_RS10025 0.58 +2.3 2,123,052 - BWI76_RS10025 0.58 +2.0 2,123,057 - BWI76_RS10025 0.59 +2.7 2,123,065 - BWI76_RS10025 0.59 +0.2 2,123,090 + BWI76_RS10025 0.61 +1.9 2,123,090 + BWI76_RS10025 0.61 +2.3 2,123,090 + BWI76_RS10025 0.61 +2.0 2,123,097 - BWI76_RS10025 0.62 +1.5 2,123,111 + BWI76_RS10025 0.63 +1.5 2,123,111 + BWI76_RS10025 0.63 +2.1 2,123,111 + BWI76_RS10025 0.63 +1.9 2,123,119 - BWI76_RS10025 0.64 +2.1 2,123,119 - BWI76_RS10025 0.64 +1.7 2,123,119 - BWI76_RS10025 0.64 +2.0 2,123,137 + BWI76_RS10025 0.65 +1.6 2,123,154 + BWI76_RS10025 0.67 +2.7 2,123,164 + BWI76_RS10025 0.68 +1.6 2,123,169 + BWI76_RS10025 0.68 +1.1 2,123,169 + BWI76_RS10025 0.68 +2.0 2,123,169 + BWI76_RS10025 0.68 +2.2 2,123,169 + BWI76_RS10025 0.68 +2.0 2,123,169 + BWI76_RS10025 0.68 +2.0 2,123,177 - BWI76_RS10025 0.69 +1.5 2,123,177 - BWI76_RS10025 0.69 +2.1 2,123,177 - BWI76_RS10025 0.69 +1.7 2,123,214 - BWI76_RS10025 0.72 +1.9 2,123,256 - BWI76_RS10025 0.75 +2.2 2,123,324 + BWI76_RS10025 0.81 +2.1 2,123,339 - BWI76_RS10025 0.82 +1.1 2,123,348 + BWI76_RS10025 0.83 +2.0 2,123,348 + BWI76_RS10025 0.83 +2.1 2,123,348 + BWI76_RS10025 0.83 +2.0 2,123,356 - BWI76_RS10025 0.83 +1.8 2,123,356 - BWI76_RS10025 0.83 +1.8 2,123,356 - BWI76_RS10025 0.83 -1.2 2,123,392 + BWI76_RS10025 0.86 +2.0 2,123,410 - BWI76_RS10025 0.88 +2.5 2,123,456 - +1.9 2,123,473 + +2.0 2,123,527 + +1.9 2,123,535 - +0.6 2,123,565 + -0.4 2,123,570 + +0.1 2,123,570 + -0.3 2,123,573 - +1.9 2,123,578 - +1.6 2,123,578 - +2.1 2,123,610 + +1.0 2,123,610 + +0.0 2,123,610 + -0.2 2,123,610 + -0.2 2,123,613 - +1.6 2,123,615 + -0.5 2,123,615 + -0.2 2,123,618 - +1.4 2,123,623 - +1.6 2,123,623 - +0.4 2,123,665 - +1.6 2,123,681 - +1.6 2,123,684 + +0.3 2,123,692 - +1.6 2,123,694 - +1.9 2,123,696 + +0.3 2,123,704 - +1.4 2,123,763 - +2.0 2,123,789 - +2.3 2,123,791 - +1.8 2,123,803 + +1.7 2,123,863 - +2.6 2,123,864 - +1.9 2,123,864 - +1.7 2,123,880 + +1.3 2,123,966 + +1.8 2,124,176 + BWI76_RS10030 0.12 +1.2 2,124,184 - BWI76_RS10030 0.13 +1.8 2,124,367 - BWI76_RS10030 0.18 +1.5 2,124,537 - BWI76_RS10030 0.24 +1.6 2,124,562 + BWI76_RS10030 0.25 +1.6 2,124,936 - BWI76_RS10030 0.36 +1.5 2,124,955 + BWI76_RS10030 0.37 +1.7 2,125,278 + BWI76_RS10030 0.47 +1.8 2,125,534 + BWI76_RS10030 0.55 +1.4 2,125,541 + BWI76_RS10030 0.56 +1.6 2,125,714 + BWI76_RS10030 0.61 +1.4 2,125,759 + BWI76_RS10030 0.63 +1.5 2,125,824 - BWI76_RS10030 0.65 +2.1 2,125,943 + BWI76_RS10030 0.68 +1.2 2,126,146 - BWI76_RS10030 0.75 +2.1 2,126,443 + BWI76_RS10030 0.84 +2.0 2,126,451 - BWI76_RS10030 0.85 +2.1 2,126,451 - +0.6 2,126,503 - BWI76_RS10030 0.86 +2.0 2,126,770 + +1.5 2,126,859 + +1.2 2,126,880 + +0.7 2,126,888 - -0.3 2,127,018 - +0.5 2,127,267 + BWI76_RS10035 0.31 -1.4 2,127,326 + BWI76_RS10035 0.38 +0.7 2,127,835 + +1.3
Or see this region's nucleotide sequence