Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09540 and BWI76_RS09545 are separated by 96 nucleotides BWI76_RS09545 and BWI76_RS09550 are separated by 10 nucleotides
BWI76_RS09540: BWI76_RS09540 - hypothetical protein, at 1,994,714 to 1,995,727
_RS09540
BWI76_RS09545: BWI76_RS09545 - NAD-dependent epimerase/dehydratase, at 1,995,824 to 1,997,257
_RS09545
BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269
_RS09550
Position (kb)
1995
1996
1997
1998 Strain fitness (log2 ratio)
-1
0
1 at 1994.934 kb on + strand, within BWI76_RS09540 at 1995.367 kb on + strand, within BWI76_RS09540 at 1995.592 kb on - strand, within BWI76_RS09540 at 1995.999 kb on - strand, within BWI76_RS09545 at 1995.999 kb on - strand, within BWI76_RS09545 at 1996.322 kb on - strand, within BWI76_RS09545 at 1996.669 kb on - strand, within BWI76_RS09545 at 1996.874 kb on + strand, within BWI76_RS09545 at 1997.060 kb on - strand, within BWI76_RS09545 at 1997.075 kb on + strand, within BWI76_RS09545 at 1997.092 kb on + strand, within BWI76_RS09545 at 1997.274 kb on - strand at 1997.370 kb on - strand, within BWI76_RS09550 at 1997.375 kb on - strand, within BWI76_RS09550 at 1997.556 kb on - strand, within BWI76_RS09550 at 1997.744 kb on - strand, within BWI76_RS09550 at 1997.750 kb on - strand, within BWI76_RS09550 at 1998.198 kb on - strand at 1998.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 1,994,934 + BWI76_RS09540 0.22 +0.2 1,995,367 + BWI76_RS09540 0.64 -0.5 1,995,592 - BWI76_RS09540 0.87 +0.1 1,995,999 - BWI76_RS09545 0.12 -0.4 1,995,999 - BWI76_RS09545 0.12 +0.1 1,996,322 - BWI76_RS09545 0.35 -1.2 1,996,669 - BWI76_RS09545 0.59 +0.0 1,996,874 + BWI76_RS09545 0.73 -1.0 1,997,060 - BWI76_RS09545 0.86 -0.3 1,997,075 + BWI76_RS09545 0.87 -0.8 1,997,092 + BWI76_RS09545 0.88 -0.6 1,997,274 - +0.6 1,997,370 - BWI76_RS09550 0.10 -0.3 1,997,375 - BWI76_RS09550 0.11 +0.1 1,997,556 - BWI76_RS09550 0.29 +0.2 1,997,744 - BWI76_RS09550 0.48 +0.4 1,997,750 - BWI76_RS09550 0.48 -1.3 1,998,198 - +0.9 1,998,235 + -0.2
Or see this region's nucleotide sequence