Experiment: RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07755 and BWI76_RS07760 overlap by 1 nucleotides BWI76_RS07760 and BWI76_RS07765 are separated by 0 nucleotides BWI76_RS07765 and BWI76_RS07770 are separated by 122 nucleotides BWI76_RS07770 and BWI76_RS07775 are separated by 39 nucleotides
BWI76_RS07755: BWI76_RS07755 - isochorismatase, at 1,646,122 to 1,646,973
_RS07755
BWI76_RS07760: BWI76_RS07760 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 1,646,973 to 1,647,728
_RS07760
BWI76_RS07765: BWI76_RS07765 - thioesterase, at 1,647,729 to 1,648,142
_RS07765
BWI76_RS07770: BWI76_RS07770 - hypothetical protein, at 1,648,265 to 1,648,485
_RS07770
BWI76_RS07775: BWI76_RS07775 - carbon starvation protein A, at 1,648,525 to 1,650,630
_RS07775
Position (kb)
1647
1648
1649 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1647.053 kb on - strand, within BWI76_RS07760 at 1647.090 kb on - strand, within BWI76_RS07760 at 1647.138 kb on - strand, within BWI76_RS07760 at 1647.365 kb on - strand, within BWI76_RS07760 at 1647.429 kb on + strand, within BWI76_RS07760 at 1647.815 kb on - strand, within BWI76_RS07765 at 1647.825 kb on - strand, within BWI76_RS07765 at 1647.873 kb on - strand, within BWI76_RS07765 at 1648.232 kb on - strand at 1648.420 kb on - strand, within BWI76_RS07770 at 1648.512 kb on + strand at 1648.553 kb on + strand at 1648.912 kb on + strand, within BWI76_RS07775 at 1649.092 kb on - strand, within BWI76_RS07775
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 1,647,053 - BWI76_RS07760 0.11 +0.3 1,647,090 - BWI76_RS07760 0.15 +0.7 1,647,138 - BWI76_RS07760 0.22 -1.5 1,647,365 - BWI76_RS07760 0.52 -0.1 1,647,429 + BWI76_RS07760 0.60 -0.2 1,647,815 - BWI76_RS07765 0.21 -0.9 1,647,825 - BWI76_RS07765 0.23 +0.1 1,647,873 - BWI76_RS07765 0.35 -1.8 1,648,232 - +0.2 1,648,420 - BWI76_RS07770 0.70 +0.3 1,648,512 + +1.1 1,648,553 + +1.9 1,648,912 + BWI76_RS07775 0.18 +0.1 1,649,092 - BWI76_RS07775 0.27 +0.3
Or see this region's nucleotide sequence