Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1980

Experiment: Varel_Bryant_medium_Glucose with 2-Mercaptopyridine N-oxide 0.1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1979 and BT1980 are separated by 146 nucleotidesBT1980 and BT1981 are separated by 111 nucleotidesBT1981 and BT1982 overlap by 4 nucleotidesBT1982 and BT1983 are separated by 2 nucleotides BT1979: BT1979 - Meso-diaminopimelate D-dehydrogenase (NCBI ptt file), at 2,489,425 to 2,490,324 BT1979 BT1980: BT1980 - transposase (NCBI ptt file), at 2,490,471 to 2,492,132 BT1980 BT1981: BT1981 - hypothetical protein (NCBI ptt file), at 2,492,244 to 2,492,549 BT1981 BT1982: BT1982 - hypothetical protein (NCBI ptt file), at 2,492,546 to 2,492,875 BT1982 BT1983: BT1983 - hemolysin III (NCBI ptt file), at 2,492,878 to 2,493,567 BT1983 Position (kb) 2490 2491 2492 2493Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 2489.473 kb on + strandat 2489.943 kb on + strand, within BT1979at 2489.973 kb on - strand, within BT1979at 2489.973 kb on - strand, within BT1979at 2489.974 kb on + strand, within BT1979at 2489.974 kb on + strandat 2489.974 kb on + strand, within BT1979at 2489.975 kb on - strand, within BT1979at 2489.976 kb on + strand, within BT1979at 2490.037 kb on + strand, within BT1979at 2490.038 kb on - strand, within BT1979at 2490.038 kb on - strand, within BT1979at 2490.204 kb on - strand, within BT1979at 2490.250 kb on + strandat 2490.260 kb on + strandat 2490.260 kb on + strandat 2490.321 kb on - strandat 2490.323 kb on - strandat 2490.427 kb on + strandat 2490.431 kb on - strandat 2490.481 kb on + strandat 2490.568 kb on + strandat 2490.569 kb on - strandat 2490.741 kb on + strand, within BT1980at 2490.743 kb on + strand, within BT1980at 2490.818 kb on - strand, within BT1980at 2490.818 kb on - strand, within BT1980at 2490.826 kb on + strand, within BT1980at 2490.827 kb on - strand, within BT1980at 2490.881 kb on - strand, within BT1980at 2490.888 kb on - strand, within BT1980at 2491.186 kb on - strand, within BT1980at 2491.325 kb on + strand, within BT1980at 2491.337 kb on - strand, within BT1980at 2491.434 kb on + strand, within BT1980at 2491.533 kb on + strand, within BT1980at 2491.534 kb on - strand, within BT1980at 2491.539 kb on + strand, within BT1980at 2491.603 kb on - strand, within BT1980at 2491.634 kb on + strand, within BT1980at 2491.655 kb on + strand, within BT1980at 2491.656 kb on - strand, within BT1980at 2491.712 kb on + strand, within BT1980at 2491.712 kb on + strand, within BT1980at 2491.775 kb on + strand, within BT1980at 2491.775 kb on + strand, within BT1980at 2491.776 kb on - strand, within BT1980at 2491.781 kb on - strand, within BT1980at 2491.985 kb on + strandat 2492.014 kb on + strandat 2492.045 kb on + strandat 2492.051 kb on + strandat 2492.057 kb on + strandat 2492.107 kb on + strandat 2492.108 kb on + strandat 2492.153 kb on - strandat 2492.159 kb on + strandat 2492.427 kb on + strand, within BT1981at 2492.427 kb on + strand, within BT1981at 2492.428 kb on - strand, within BT1981at 2492.431 kb on - strand, within BT1981at 2492.431 kb on - strand, within BT1981at 2492.482 kb on + strand, within BT1981at 2492.537 kb on - strandat 2492.553 kb on + strandat 2492.604 kb on + strand, within BT1982at 2492.607 kb on + strand, within BT1982at 2492.724 kb on + strand, within BT1982at 2492.726 kb on + strand, within BT1982at 2492.727 kb on - strand, within BT1982at 2492.937 kb on + strandat 2492.937 kb on + strandat 2492.938 kb on - strandat 2492.979 kb on + strand, within BT1983at 2492.982 kb on - strand, within BT1983at 2493.062 kb on + strand, within BT1983at 2493.094 kb on + strand, within BT1983at 2493.113 kb on - strand, within BT1983

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant_medium_Glucose with 2-Mercaptopyridine N-oxide 0.1 mM
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2,489,473 + -4.2
2,489,943 + BT1979 0.58 -2.0
2,489,973 - BT1979 0.61 -2.6
2,489,973 - BT1979 0.61 -1.8
2,489,974 + BT1979 0.61 -2.0
2,489,974 + -0.0
2,489,974 + BT1979 0.61 -0.0
2,489,975 - BT1979 0.61 -2.5
2,489,976 + BT1979 0.61 -3.2
2,490,037 + BT1979 0.68 -2.5
2,490,038 - BT1979 0.68 -3.8
2,490,038 - BT1979 0.68 -0.0
2,490,204 - BT1979 0.87 -0.0
2,490,250 + -2.1
2,490,260 + -0.0
2,490,260 + -3.0
2,490,321 - -2.8
2,490,323 - -1.0
2,490,427 + -3.0
2,490,431 - -0.0
2,490,481 + -0.3
2,490,568 + -1.0
2,490,569 - -0.3
2,490,741 + BT1980 0.16 -0.8
2,490,743 + BT1980 0.16 +1.0
2,490,818 - BT1980 0.21 +0.6
2,490,818 - BT1980 0.21 -1.0
2,490,826 + BT1980 0.21 +0.6
2,490,827 - BT1980 0.21 -0.0
2,490,881 - BT1980 0.25 -0.0
2,490,888 - BT1980 0.25 -1.3
2,491,186 - BT1980 0.43 +0.7
2,491,325 + BT1980 0.51 -1.8
2,491,337 - BT1980 0.52 -1.0
2,491,434 + BT1980 0.58 -0.6
2,491,533 + BT1980 0.64 -1.0
2,491,534 - BT1980 0.64 +0.3
2,491,539 + BT1980 0.64 -1.0
2,491,603 - BT1980 0.68 -0.0
2,491,634 + BT1980 0.70 +0.4
2,491,655 + BT1980 0.71 -1.6
2,491,656 - BT1980 0.71 -2.6
2,491,712 + BT1980 0.75 -1.6
2,491,712 + BT1980 0.75 +1.0
2,491,775 + BT1980 0.78 +1.3
2,491,775 + BT1980 0.78 -0.0
2,491,776 - BT1980 0.79 +1.0
2,491,781 - BT1980 0.79 -1.9
2,491,985 + +1.6
2,492,014 + -0.0
2,492,045 + -2.5
2,492,051 + -0.8
2,492,057 + -0.6
2,492,107 + -0.9
2,492,108 + -0.0
2,492,153 - -1.6
2,492,159 + -0.6
2,492,427 + BT1981 0.60 +1.7
2,492,427 + BT1981 0.60 -0.0
2,492,428 - BT1981 0.60 -0.0
2,492,431 - BT1981 0.61 -1.5
2,492,431 - BT1981 0.61 -4.2
2,492,482 + BT1981 0.78 -1.1
2,492,537 - -1.0
2,492,553 + -0.0
2,492,604 + BT1982 0.18 -1.6
2,492,607 + BT1982 0.18 -2.1
2,492,724 + BT1982 0.54 -0.3
2,492,726 + BT1982 0.55 -1.6
2,492,727 - BT1982 0.55 -0.0
2,492,937 + -2.6
2,492,937 + -2.0
2,492,938 - -2.6
2,492,979 + BT1983 0.15 -5.9
2,492,982 - BT1983 0.15 -2.6
2,493,062 + BT1983 0.27 -3.4
2,493,094 + BT1983 0.31 -2.4
2,493,113 - BT1983 0.34 -2.6

Or see this region's nucleotide sequence