Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3488 and Psyr_3489 are separated by 49 nucleotides Psyr_3489 and Psyr_3490 are separated by 82 nucleotides Psyr_3490 and Psyr_3491 are separated by 62 nucleotides
Psyr_3488: Psyr_3488 - anti-sigma-28 factor, FlgM, at 4,157,659 to 4,157,973
_3488
Psyr_3489: Psyr_3489 - FlgN, at 4,158,023 to 4,158,490
_3489
Psyr_3490: Psyr_3490 - conserved hypothetical protein, at 4,158,573 to 4,159,319
_3490
Psyr_3491: Psyr_3491 - Major facilitator superfamily, at 4,159,382 to 4,160,803
_3491
Position (kb)
4158
4159
4160 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4157.971 kb on + strand at 4157.972 kb on - strand at 4158.034 kb on + strand at 4158.034 kb on + strand at 4158.035 kb on - strand at 4158.035 kb on - strand at 4158.035 kb on - strand at 4158.203 kb on - strand, within Psyr_3489 at 4158.276 kb on + strand, within Psyr_3489 at 4158.424 kb on + strand, within Psyr_3489 at 4158.424 kb on + strand, within Psyr_3489 at 4158.424 kb on + strand, within Psyr_3489 at 4158.424 kb on + strand, within Psyr_3489 at 4158.425 kb on - strand, within Psyr_3489 at 4158.432 kb on - strand, within Psyr_3489 at 4158.432 kb on - strand, within Psyr_3489 at 4158.432 kb on - strand, within Psyr_3489 at 4158.457 kb on + strand at 4158.489 kb on - strand at 4158.499 kb on - strand at 4158.547 kb on - strand at 4158.549 kb on + strand at 4158.549 kb on + strand at 4158.623 kb on + strand at 4158.623 kb on + strand at 4158.624 kb on - strand at 4158.624 kb on - strand at 4158.624 kb on - strand at 4158.624 kb on - strand at 4158.624 kb on - strand at 4158.686 kb on + strand, within Psyr_3490 at 4158.687 kb on - strand, within Psyr_3490 at 4158.687 kb on - strand, within Psyr_3490 at 4158.720 kb on + strand, within Psyr_3490 at 4158.720 kb on + strand, within Psyr_3490 at 4158.721 kb on - strand, within Psyr_3490 at 4158.721 kb on - strand, within Psyr_3490 at 4158.860 kb on + strand, within Psyr_3490 at 4158.861 kb on - strand, within Psyr_3490 at 4158.861 kb on - strand, within Psyr_3490 at 4158.903 kb on + strand, within Psyr_3490 at 4158.904 kb on - strand, within Psyr_3490 at 4158.904 kb on - strand, within Psyr_3490 at 4158.934 kb on - strand, within Psyr_3490 at 4159.044 kb on - strand, within Psyr_3490 at 4159.111 kb on + strand, within Psyr_3490 at 4159.111 kb on + strand, within Psyr_3490 at 4159.113 kb on + strand, within Psyr_3490 at 4159.114 kb on - strand, within Psyr_3490 at 4159.114 kb on - strand, within Psyr_3490 at 4159.114 kb on - strand, within Psyr_3490 at 4159.114 kb on - strand, within Psyr_3490 at 4159.114 kb on - strand, within Psyr_3490 at 4159.269 kb on + strand at 4159.276 kb on + strand at 4159.276 kb on + strand at 4159.277 kb on - strand at 4159.377 kb on - strand at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.618 kb on + strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.619 kb on - strand, within Psyr_3491 at 4159.734 kb on + strand, within Psyr_3491 at 4159.734 kb on + strand, within Psyr_3491 at 4159.735 kb on - strand, within Psyr_3491 at 4159.806 kb on + strand, within Psyr_3491 at 4159.806 kb on + strand, within Psyr_3491 at 4159.807 kb on - strand, within Psyr_3491 at 4159.807 kb on - strand, within Psyr_3491 at 4159.807 kb on - strand, within Psyr_3491 at 4160.002 kb on + strand, within Psyr_3491 at 4160.002 kb on + strand, within Psyr_3491 at 4160.002 kb on + strand, within Psyr_3491 at 4160.003 kb on - strand, within Psyr_3491 at 4160.003 kb on - strand, within Psyr_3491 at 4160.134 kb on - strand, within Psyr_3491 at 4160.183 kb on + strand, within Psyr_3491
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 4,157,971 + +4.0 4,157,972 - -1.3 4,158,034 + -1.5 4,158,034 + -2.9 4,158,035 - -2.5 4,158,035 - +0.3 4,158,035 - -1.5 4,158,203 - Psyr_3489 0.38 +0.6 4,158,276 + Psyr_3489 0.54 -1.1 4,158,424 + Psyr_3489 0.86 -0.9 4,158,424 + Psyr_3489 0.86 -0.9 4,158,424 + Psyr_3489 0.86 -1.8 4,158,424 + Psyr_3489 0.86 -0.3 4,158,425 - Psyr_3489 0.86 -2.6 4,158,432 - Psyr_3489 0.87 -1.4 4,158,432 - Psyr_3489 0.87 -1.5 4,158,432 - Psyr_3489 0.87 -3.5 4,158,457 + -0.5 4,158,489 - +1.0 4,158,499 - -0.8 4,158,547 - +0.8 4,158,549 + -0.9 4,158,549 + -0.1 4,158,623 + -1.8 4,158,623 + +0.9 4,158,624 - +0.2 4,158,624 - -1.3 4,158,624 - +2.1 4,158,624 - +1.0 4,158,624 - +0.0 4,158,686 + Psyr_3490 0.15 -0.4 4,158,687 - Psyr_3490 0.15 -0.2 4,158,687 - Psyr_3490 0.15 +1.3 4,158,720 + Psyr_3490 0.20 -1.5 4,158,720 + Psyr_3490 0.20 -0.4 4,158,721 - Psyr_3490 0.20 -0.3 4,158,721 - Psyr_3490 0.20 +0.8 4,158,860 + Psyr_3490 0.38 +2.1 4,158,861 - Psyr_3490 0.39 +0.3 4,158,861 - Psyr_3490 0.39 +2.0 4,158,903 + Psyr_3490 0.44 +1.1 4,158,904 - Psyr_3490 0.44 -0.1 4,158,904 - Psyr_3490 0.44 -1.4 4,158,934 - Psyr_3490 0.48 -0.8 4,159,044 - Psyr_3490 0.63 +1.0 4,159,111 + Psyr_3490 0.72 +0.4 4,159,111 + Psyr_3490 0.72 -0.7 4,159,113 + Psyr_3490 0.72 +0.6 4,159,114 - Psyr_3490 0.72 -0.3 4,159,114 - Psyr_3490 0.72 -0.0 4,159,114 - Psyr_3490 0.72 +0.9 4,159,114 - Psyr_3490 0.72 -0.8 4,159,114 - Psyr_3490 0.72 +1.4 4,159,269 + -0.6 4,159,276 + +1.8 4,159,276 + -0.2 4,159,277 - +0.8 4,159,377 - +0.2 4,159,618 + Psyr_3491 0.17 +0.0 4,159,618 + Psyr_3491 0.17 +0.3 4,159,618 + Psyr_3491 0.17 -0.3 4,159,618 + Psyr_3491 0.17 -0.4 4,159,618 + Psyr_3491 0.17 +1.3 4,159,618 + Psyr_3491 0.17 -0.6 4,159,618 + Psyr_3491 0.17 -0.1 4,159,618 + Psyr_3491 0.17 -0.5 4,159,619 - Psyr_3491 0.17 -1.2 4,159,619 - Psyr_3491 0.17 +0.2 4,159,619 - Psyr_3491 0.17 +0.6 4,159,619 - Psyr_3491 0.17 -1.5 4,159,619 - Psyr_3491 0.17 +0.5 4,159,619 - Psyr_3491 0.17 +0.5 4,159,734 + Psyr_3491 0.25 -0.3 4,159,734 + Psyr_3491 0.25 +0.4 4,159,735 - Psyr_3491 0.25 +1.2 4,159,806 + Psyr_3491 0.30 +0.6 4,159,806 + Psyr_3491 0.30 +0.6 4,159,807 - Psyr_3491 0.30 +0.8 4,159,807 - Psyr_3491 0.30 +2.1 4,159,807 - Psyr_3491 0.30 +0.3 4,160,002 + Psyr_3491 0.44 -0.9 4,160,002 + Psyr_3491 0.44 +0.8 4,160,002 + Psyr_3491 0.44 +0.7 4,160,003 - Psyr_3491 0.44 -0.9 4,160,003 - Psyr_3491 0.44 +2.7 4,160,134 - Psyr_3491 0.53 -2.3 4,160,183 + Psyr_3491 0.56 +0.9
Or see this region's nucleotide sequence