Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2988 and Psyr_2989 overlap by 1 nucleotides Psyr_2989 and Psyr_2990 are separated by 312 nucleotides Psyr_2990 and Psyr_2991 are separated by 160 nucleotides
Psyr_2988: Psyr_2988 - glucose-6-phosphate 1-dehydrogenase, at 3,579,407 to 3,580,939
_2988
Psyr_2989: Psyr_2989 - 6-phosphogluconate dehydrogenase (decarboxylating), at 3,580,939 to 3,581,913
_2989
Psyr_2990: Psyr_2990 - conserved hypothetical protein, at 3,582,226 to 3,582,399
_2990
Psyr_2991: Psyr_2991 - GCN5-related N-acetyltransferase, at 3,582,560 to 3,583,051
_2991
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3580.015 kb on - strand, within Psyr_2988 at 3580.226 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.263 kb on + strand, within Psyr_2988 at 3580.264 kb on - strand, within Psyr_2988 at 3580.264 kb on - strand, within Psyr_2988 at 3580.596 kb on + strand, within Psyr_2988 at 3580.597 kb on - strand, within Psyr_2988 at 3580.597 kb on - strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.629 kb on + strand, within Psyr_2988 at 3580.630 kb on - strand, within Psyr_2988 at 3580.630 kb on - strand, within Psyr_2988 at 3580.671 kb on - strand, within Psyr_2988 at 3580.824 kb on + strand at 3580.940 kb on + strand at 3580.940 kb on + strand at 3580.940 kb on + strand at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.387 kb on + strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.523 kb on - strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.570 kb on + strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.571 kb on - strand, within Psyr_2989 at 3581.621 kb on + strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.622 kb on - strand, within Psyr_2989 at 3581.828 kb on + strand at 3581.829 kb on - strand at 3581.829 kb on - strand at 3581.926 kb on + strand at 3581.926 kb on + strand at 3581.927 kb on - strand at 3581.988 kb on - strand at 3582.071 kb on - strand at 3582.149 kb on - strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.236 kb on + strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.237 kb on - strand at 3582.239 kb on + strand at 3582.239 kb on + strand at 3582.265 kb on + strand, within Psyr_2990 at 3582.299 kb on + strand, within Psyr_2990 at 3582.300 kb on - strand, within Psyr_2990 at 3582.409 kb on + strand at 3582.409 kb on + strand at 3582.409 kb on + strand at 3582.561 kb on + strand at 3582.612 kb on + strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.613 kb on - strand, within Psyr_2991 at 3582.883 kb on - strand, within Psyr_2991
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,580,015 - Psyr_2988 0.40 -0.1 3,580,226 + Psyr_2988 0.53 -0.4 3,580,263 + Psyr_2988 0.56 -1.5 3,580,263 + Psyr_2988 0.56 +0.5 3,580,263 + Psyr_2988 0.56 +0.1 3,580,264 - Psyr_2988 0.56 -1.3 3,580,264 - Psyr_2988 0.56 -1.6 3,580,596 + Psyr_2988 0.78 -1.2 3,580,597 - Psyr_2988 0.78 -1.9 3,580,597 - Psyr_2988 0.78 -3.1 3,580,629 + Psyr_2988 0.80 -0.3 3,580,629 + Psyr_2988 0.80 -0.2 3,580,629 + Psyr_2988 0.80 +0.9 3,580,630 - Psyr_2988 0.80 -0.1 3,580,630 - Psyr_2988 0.80 -0.1 3,580,671 - Psyr_2988 0.82 -0.5 3,580,824 + +1.9 3,580,940 + -0.2 3,580,940 + +1.4 3,580,940 + -0.7 3,581,387 + Psyr_2989 0.46 -1.0 3,581,387 + Psyr_2989 0.46 +0.6 3,581,387 + Psyr_2989 0.46 -2.7 3,581,387 + Psyr_2989 0.46 +1.6 3,581,523 - Psyr_2989 0.60 -0.2 3,581,523 - Psyr_2989 0.60 +1.3 3,581,570 + Psyr_2989 0.65 +4.3 3,581,570 + Psyr_2989 0.65 -1.9 3,581,570 + Psyr_2989 0.65 +1.1 3,581,570 + Psyr_2989 0.65 -0.1 3,581,571 - Psyr_2989 0.65 -0.2 3,581,571 - Psyr_2989 0.65 +1.5 3,581,571 - Psyr_2989 0.65 +1.6 3,581,571 - Psyr_2989 0.65 -1.7 3,581,621 + Psyr_2989 0.70 +1.0 3,581,622 - Psyr_2989 0.70 +1.8 3,581,622 - Psyr_2989 0.70 +0.0 3,581,622 - Psyr_2989 0.70 -1.2 3,581,622 - Psyr_2989 0.70 -1.0 3,581,828 + -0.2 3,581,829 - -1.5 3,581,829 - -1.3 3,581,926 + +0.6 3,581,926 + +1.2 3,581,927 - -1.9 3,581,988 - -0.6 3,582,071 - -1.4 3,582,149 - +0.7 3,582,236 + +0.2 3,582,236 + +0.1 3,582,236 + -0.9 3,582,236 + -0.2 3,582,236 + +1.2 3,582,236 + -0.9 3,582,236 + +0.4 3,582,237 - +0.9 3,582,237 - +0.5 3,582,237 - -0.3 3,582,237 - +2.5 3,582,237 - +0.6 3,582,239 + -0.3 3,582,239 + -0.5 3,582,265 + Psyr_2990 0.22 +0.2 3,582,299 + Psyr_2990 0.42 -1.1 3,582,300 - Psyr_2990 0.43 +0.3 3,582,409 + +0.5 3,582,409 + -0.7 3,582,409 + -1.8 3,582,561 + -1.2 3,582,612 + Psyr_2991 0.11 -1.0 3,582,613 - Psyr_2991 0.11 +0.3 3,582,613 - Psyr_2991 0.11 -0.1 3,582,613 - Psyr_2991 0.11 -2.0 3,582,613 - Psyr_2991 0.11 -0.6 3,582,613 - Psyr_2991 0.11 -1.0 3,582,613 - Psyr_2991 0.11 -0.3 3,582,883 - Psyr_2991 0.66 +0.1
Or see this region's nucleotide sequence