Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2402 and Psyr_2403 are separated by 94 nucleotides Psyr_2403 and Psyr_2404 are separated by 32 nucleotides Psyr_2404 and Psyr_2405 are separated by 1 nucleotides Psyr_2405 and Psyr_2406 are separated by 175 nucleotides
Psyr_2402: Psyr_2402 - 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II, at 2,784,425 to 2,785,534
_2402
Psyr_2403: Psyr_2403 - Protein of unknown function DUF1330, at 2,785,629 to 2,785,919
_2403
Psyr_2404: Psyr_2404 - NUDIX hydrolase, at 2,785,952 to 2,786,515
_2404
Psyr_2405: Psyr_2405 - transcriptional regulator, GntR family, at 2,786,517 to 2,787,269
_2405
Psyr_2406: Psyr_2406 - Flavin reductase-like protein, at 2,787,445 to 2,787,930
_2406
Position (kb)
2785
2786
2787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2785.156 kb on + strand, within Psyr_2402 at 2785.157 kb on - strand, within Psyr_2402 at 2785.638 kb on + strand at 2785.638 kb on + strand at 2785.677 kb on + strand, within Psyr_2403 at 2785.677 kb on + strand, within Psyr_2403 at 2785.677 kb on + strand, within Psyr_2403 at 2785.678 kb on - strand, within Psyr_2403 at 2785.678 kb on - strand, within Psyr_2403 at 2785.686 kb on + strand, within Psyr_2403 at 2785.687 kb on - strand, within Psyr_2403 at 2785.833 kb on + strand, within Psyr_2403 at 2785.834 kb on - strand, within Psyr_2403 at 2785.995 kb on + strand at 2785.995 kb on + strand at 2785.995 kb on + strand at 2785.995 kb on + strand at 2785.995 kb on + strand at 2785.996 kb on - strand at 2785.996 kb on - strand at 2785.996 kb on - strand at 2786.000 kb on - strand at 2786.005 kb on - strand at 2786.031 kb on + strand, within Psyr_2404 at 2786.031 kb on + strand, within Psyr_2404 at 2786.031 kb on + strand, within Psyr_2404 at 2786.032 kb on - strand, within Psyr_2404 at 2786.042 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.064 kb on + strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.065 kb on - strand, within Psyr_2404 at 2786.169 kb on + strand, within Psyr_2404 at 2786.169 kb on + strand, within Psyr_2404 at 2786.169 kb on + strand, within Psyr_2404 at 2786.170 kb on - strand, within Psyr_2404 at 2786.170 kb on - strand, within Psyr_2404 at 2786.170 kb on - strand, within Psyr_2404 at 2786.205 kb on + strand, within Psyr_2404 at 2786.228 kb on + strand, within Psyr_2404 at 2786.228 kb on + strand, within Psyr_2404 at 2786.352 kb on + strand, within Psyr_2404 at 2786.352 kb on + strand, within Psyr_2404 at 2786.352 kb on + strand, within Psyr_2404 at 2786.352 kb on + strand, within Psyr_2404 at 2786.353 kb on - strand, within Psyr_2404 at 2786.353 kb on - strand, within Psyr_2404 at 2786.353 kb on - strand, within Psyr_2404 at 2786.353 kb on - strand, within Psyr_2404 at 2786.369 kb on + strand, within Psyr_2404 at 2786.403 kb on + strand, within Psyr_2404 at 2786.404 kb on - strand, within Psyr_2404 at 2786.412 kb on + strand, within Psyr_2404 at 2786.413 kb on - strand, within Psyr_2404 at 2786.413 kb on - strand, within Psyr_2404 at 2786.413 kb on - strand, within Psyr_2404 at 2786.526 kb on + strand at 2786.526 kb on + strand at 2786.527 kb on - strand at 2786.527 kb on - strand at 2786.527 kb on - strand at 2786.527 kb on - strand at 2786.603 kb on - strand, within Psyr_2405 at 2786.631 kb on + strand, within Psyr_2405 at 2786.631 kb on + strand, within Psyr_2405 at 2786.632 kb on - strand, within Psyr_2405 at 2786.632 kb on - strand, within Psyr_2405 at 2786.758 kb on + strand, within Psyr_2405 at 2786.758 kb on + strand, within Psyr_2405 at 2786.826 kb on + strand, within Psyr_2405 at 2786.826 kb on + strand, within Psyr_2405 at 2786.826 kb on + strand, within Psyr_2405 at 2786.826 kb on + strand, within Psyr_2405 at 2786.826 kb on + strand, within Psyr_2405 at 2786.827 kb on - strand, within Psyr_2405 at 2786.827 kb on - strand, within Psyr_2405 at 2786.827 kb on - strand, within Psyr_2405 at 2786.827 kb on - strand, within Psyr_2405 at 2786.827 kb on - strand, within Psyr_2405 at 2786.833 kb on + strand, within Psyr_2405 at 2786.833 kb on + strand, within Psyr_2405 at 2786.985 kb on + strand, within Psyr_2405 at 2786.986 kb on - strand, within Psyr_2405 at 2786.986 kb on - strand, within Psyr_2405 at 2787.317 kb on + strand at 2787.485 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,785,156 + Psyr_2402 0.66 +0.8 2,785,157 - Psyr_2402 0.66 -1.0 2,785,638 + +2.0 2,785,638 + -3.0 2,785,677 + Psyr_2403 0.16 -0.0 2,785,677 + Psyr_2403 0.16 -0.4 2,785,677 + Psyr_2403 0.16 -1.1 2,785,678 - Psyr_2403 0.17 -0.7 2,785,678 - Psyr_2403 0.17 -0.2 2,785,686 + Psyr_2403 0.20 -1.3 2,785,687 - Psyr_2403 0.20 -0.4 2,785,833 + Psyr_2403 0.70 -0.3 2,785,834 - Psyr_2403 0.70 -0.2 2,785,995 + -0.6 2,785,995 + -0.9 2,785,995 + -1.7 2,785,995 + +0.9 2,785,995 + +0.5 2,785,996 - -0.7 2,785,996 - +0.9 2,785,996 - -0.3 2,786,000 - -1.4 2,786,005 - -0.1 2,786,031 + Psyr_2404 0.14 -0.1 2,786,031 + Psyr_2404 0.14 +0.1 2,786,031 + Psyr_2404 0.14 +0.9 2,786,032 - Psyr_2404 0.14 +0.3 2,786,042 + Psyr_2404 0.16 -1.6 2,786,064 + Psyr_2404 0.20 -0.3 2,786,064 + Psyr_2404 0.20 +0.2 2,786,064 + Psyr_2404 0.20 +0.2 2,786,064 + Psyr_2404 0.20 -1.4 2,786,064 + Psyr_2404 0.20 +0.2 2,786,064 + Psyr_2404 0.20 -1.1 2,786,064 + Psyr_2404 0.20 +0.8 2,786,064 + Psyr_2404 0.20 -0.7 2,786,064 + Psyr_2404 0.20 -1.9 2,786,064 + Psyr_2404 0.20 +0.6 2,786,065 - Psyr_2404 0.20 -0.2 2,786,065 - Psyr_2404 0.20 -0.6 2,786,065 - Psyr_2404 0.20 +1.1 2,786,065 - Psyr_2404 0.20 +0.3 2,786,065 - Psyr_2404 0.20 +0.6 2,786,065 - Psyr_2404 0.20 -0.4 2,786,065 - Psyr_2404 0.20 -0.7 2,786,065 - Psyr_2404 0.20 -0.4 2,786,169 + Psyr_2404 0.38 -0.1 2,786,169 + Psyr_2404 0.38 +3.2 2,786,169 + Psyr_2404 0.38 +0.9 2,786,170 - Psyr_2404 0.39 -0.4 2,786,170 - Psyr_2404 0.39 -0.5 2,786,170 - Psyr_2404 0.39 +0.7 2,786,205 + Psyr_2404 0.45 +0.5 2,786,228 + Psyr_2404 0.49 -1.2 2,786,228 + Psyr_2404 0.49 -0.5 2,786,352 + Psyr_2404 0.71 +0.4 2,786,352 + Psyr_2404 0.71 +0.7 2,786,352 + Psyr_2404 0.71 +1.5 2,786,352 + Psyr_2404 0.71 -0.4 2,786,353 - Psyr_2404 0.71 +1.7 2,786,353 - Psyr_2404 0.71 +2.1 2,786,353 - Psyr_2404 0.71 -0.9 2,786,353 - Psyr_2404 0.71 -0.1 2,786,369 + Psyr_2404 0.74 +0.4 2,786,403 + Psyr_2404 0.80 +1.5 2,786,404 - Psyr_2404 0.80 -0.5 2,786,412 + Psyr_2404 0.82 -0.6 2,786,413 - Psyr_2404 0.82 +2.7 2,786,413 - Psyr_2404 0.82 +0.4 2,786,413 - Psyr_2404 0.82 -1.9 2,786,526 + +0.0 2,786,526 + -1.5 2,786,527 - -1.0 2,786,527 - +0.3 2,786,527 - -0.1 2,786,527 - -0.8 2,786,603 - Psyr_2405 0.11 -0.3 2,786,631 + Psyr_2405 0.15 -2.1 2,786,631 + Psyr_2405 0.15 -0.0 2,786,632 - Psyr_2405 0.15 -1.2 2,786,632 - Psyr_2405 0.15 +0.1 2,786,758 + Psyr_2405 0.32 +0.4 2,786,758 + Psyr_2405 0.32 -0.6 2,786,826 + Psyr_2405 0.41 -2.0 2,786,826 + Psyr_2405 0.41 -1.1 2,786,826 + Psyr_2405 0.41 -0.3 2,786,826 + Psyr_2405 0.41 -1.7 2,786,826 + Psyr_2405 0.41 -2.0 2,786,827 - Psyr_2405 0.41 -1.5 2,786,827 - Psyr_2405 0.41 -0.5 2,786,827 - Psyr_2405 0.41 -0.2 2,786,827 - Psyr_2405 0.41 -0.7 2,786,827 - Psyr_2405 0.41 +0.1 2,786,833 + Psyr_2405 0.42 -0.8 2,786,833 + Psyr_2405 0.42 +0.7 2,786,985 + Psyr_2405 0.62 -0.9 2,786,986 - Psyr_2405 0.62 -1.5 2,786,986 - Psyr_2405 0.62 -0.6 2,787,317 + -0.5 2,787,485 + +1.8
Or see this region's nucleotide sequence