Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1958 and Psyr_1959 are separated by 14 nucleotides Psyr_1959 and Psyr_1960 overlap by 20 nucleotides
Psyr_1958: Psyr_1958 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,244,792 to 2,252,792
_1958
Psyr_1959: Psyr_1959 - Amino acid adenylation, at 2,252,807 to 2,259,271
_1959
Psyr_1960: Psyr_1960 - Non-ribosomal peptide synthase:Amino acid adenylation, at 2,259,252 to 2,267,903
_1960
Position (kb)
2252
2253
2254
2255
2256
2257
2258
2259
2260 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2251.825 kb on - strand, within Psyr_1958 at 2252.137 kb on - strand at 2252.325 kb on - strand at 2252.356 kb on - strand at 2252.356 kb on - strand at 2252.356 kb on - strand at 2253.006 kb on - strand at 2253.209 kb on + strand at 2253.225 kb on + strand at 2253.225 kb on + strand at 2253.225 kb on + strand at 2253.226 kb on - strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.300 kb on + strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.301 kb on - strand at 2253.377 kb on + strand at 2253.575 kb on + strand, within Psyr_1959 at 2253.575 kb on + strand, within Psyr_1959 at 2253.723 kb on + strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2253.724 kb on - strand, within Psyr_1959 at 2254.115 kb on + strand, within Psyr_1959 at 2254.116 kb on - strand, within Psyr_1959 at 2254.211 kb on - strand, within Psyr_1959 at 2254.734 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2254.743 kb on - strand, within Psyr_1959 at 2255.486 kb on + strand, within Psyr_1959 at 2255.487 kb on - strand, within Psyr_1959 at 2255.706 kb on - strand, within Psyr_1959 at 2256.161 kb on + strand, within Psyr_1959 at 2256.161 kb on + strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2256.162 kb on - strand, within Psyr_1959 at 2257.685 kb on + strand, within Psyr_1959 at 2257.685 kb on + strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.686 kb on - strand, within Psyr_1959 at 2257.728 kb on - strand, within Psyr_1959 at 2257.728 kb on - strand, within Psyr_1959 at 2257.851 kb on - strand, within Psyr_1959 at 2257.898 kb on + strand, within Psyr_1959 at 2257.899 kb on - strand, within Psyr_1959 at 2257.908 kb on - strand, within Psyr_1959 at 2258.251 kb on - strand, within Psyr_1959 at 2258.251 kb on - strand, within Psyr_1959 at 2258.321 kb on + strand, within Psyr_1959 at 2258.330 kb on + strand, within Psyr_1959 at 2258.486 kb on + strand, within Psyr_1959 at 2258.486 kb on + strand, within Psyr_1959 at 2258.487 kb on - strand, within Psyr_1959 at 2258.591 kb on + strand, within Psyr_1959 at 2258.787 kb on - strand at 2258.861 kb on + strand at 2258.973 kb on - strand at 2259.420 kb on - strand at 2259.432 kb on + strand at 2259.615 kb on + strand at 2259.615 kb on + strand at 2259.778 kb on + strand at 2259.779 kb on - strand at 2259.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,251,825 - Psyr_1958 0.88 -1.5 2,252,137 - +0.2 2,252,325 - +2.0 2,252,356 - -0.9 2,252,356 - -2.4 2,252,356 - +0.8 2,253,006 - +0.5 2,253,209 + +0.6 2,253,225 + +0.7 2,253,225 + +0.9 2,253,225 + +0.9 2,253,226 - -1.8 2,253,300 + -0.7 2,253,300 + -1.1 2,253,300 + +0.2 2,253,300 + +0.8 2,253,301 - +1.8 2,253,301 - +2.4 2,253,301 - -1.6 2,253,301 - -2.3 2,253,377 + +0.7 2,253,575 + Psyr_1959 0.12 -1.2 2,253,575 + Psyr_1959 0.12 -1.6 2,253,723 + Psyr_1959 0.14 +0.1 2,253,724 - Psyr_1959 0.14 -0.6 2,253,724 - Psyr_1959 0.14 +0.9 2,253,724 - Psyr_1959 0.14 +0.8 2,254,115 + Psyr_1959 0.20 +0.9 2,254,116 - Psyr_1959 0.20 +0.2 2,254,211 - Psyr_1959 0.22 -0.3 2,254,734 - Psyr_1959 0.30 +0.4 2,254,743 - Psyr_1959 0.30 +0.0 2,254,743 - Psyr_1959 0.30 +0.4 2,254,743 - Psyr_1959 0.30 -0.1 2,255,486 + Psyr_1959 0.41 -0.3 2,255,487 - Psyr_1959 0.41 +1.2 2,255,706 - Psyr_1959 0.45 +1.3 2,256,161 + Psyr_1959 0.52 -0.4 2,256,161 + Psyr_1959 0.52 -1.0 2,256,162 - Psyr_1959 0.52 -1.3 2,256,162 - Psyr_1959 0.52 -0.3 2,256,162 - Psyr_1959 0.52 -0.7 2,257,685 + Psyr_1959 0.75 +0.4 2,257,685 + Psyr_1959 0.75 -0.9 2,257,686 - Psyr_1959 0.75 -0.4 2,257,686 - Psyr_1959 0.75 -2.0 2,257,686 - Psyr_1959 0.75 +1.5 2,257,728 - Psyr_1959 0.76 +0.8 2,257,728 - Psyr_1959 0.76 +0.8 2,257,851 - Psyr_1959 0.78 +0.1 2,257,898 + Psyr_1959 0.79 -2.3 2,257,899 - Psyr_1959 0.79 -1.5 2,257,908 - Psyr_1959 0.79 +4.1 2,258,251 - Psyr_1959 0.84 -0.8 2,258,251 - Psyr_1959 0.84 +1.9 2,258,321 + Psyr_1959 0.85 -0.2 2,258,330 + Psyr_1959 0.85 +1.3 2,258,486 + Psyr_1959 0.88 -1.0 2,258,486 + Psyr_1959 0.88 +0.7 2,258,487 - Psyr_1959 0.88 +0.3 2,258,591 + Psyr_1959 0.89 +1.1 2,258,787 - +0.9 2,258,861 + +0.4 2,258,973 - +1.0 2,259,420 - -0.4 2,259,432 + -0.4 2,259,615 + -1.1 2,259,615 + +3.0 2,259,778 + +0.8 2,259,779 - +0.7 2,259,779 - -0.5
Or see this region's nucleotide sequence