Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1923 and Psyr_1924 overlap by 26 nucleotides Psyr_1924 and Psyr_1925 are separated by 29 nucleotides Psyr_1925 and Psyr_1926 overlap by 1 nucleotides Psyr_1926 and Psyr_1927 are separated by 7 nucleotides
Psyr_1923: Psyr_1923 - hypothetical protein, at 2,196,210 to 2,197,433
_1923
Psyr_1924: Psyr_1924 - hypothetical protein, at 2,197,408 to 2,198,067
_1924
Psyr_1925: Psyr_1925 - diaminobutyrate aminotransferase apoenzyme, at 2,198,097 to 2,199,377
_1925
Psyr_1926: Psyr_1926 - hypothetical protein, at 2,199,377 to 2,200,087
_1926
Psyr_1927: Psyr_1927 - hypothetical protein, at 2,200,095 to 2,200,541
_1927
Position (kb)
2198
2199
2200 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2197.185 kb on - strand, within Psyr_1923 at 2197.226 kb on + strand, within Psyr_1923 at 2197.279 kb on + strand, within Psyr_1923 at 2197.331 kb on - strand at 2197.381 kb on - strand at 2197.383 kb on - strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.386 kb on + strand at 2197.387 kb on - strand at 2197.387 kb on - strand at 2197.461 kb on + strand at 2197.461 kb on + strand at 2197.469 kb on + strand at 2197.469 kb on + strand at 2197.470 kb on - strand at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.526 kb on - strand, within Psyr_1924 at 2197.539 kb on - strand, within Psyr_1924 at 2197.600 kb on + strand, within Psyr_1924 at 2197.800 kb on + strand, within Psyr_1924 at 2197.855 kb on + strand, within Psyr_1924 at 2197.884 kb on + strand, within Psyr_1924 at 2197.885 kb on - strand, within Psyr_1924 at 2197.902 kb on - strand, within Psyr_1924 at 2197.977 kb on + strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2197.978 kb on - strand, within Psyr_1924 at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.047 kb on + strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.048 kb on - strand at 2198.066 kb on - strand at 2198.070 kb on + strand at 2198.118 kb on + strand at 2198.118 kb on + strand at 2198.119 kb on - strand at 2198.119 kb on - strand at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.253 kb on + strand, within Psyr_1925 at 2198.295 kb on + strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.296 kb on - strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.297 kb on + strand, within Psyr_1925 at 2198.298 kb on - strand, within Psyr_1925 at 2198.378 kb on + strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.404 kb on - strand, within Psyr_1925 at 2198.579 kb on - strand, within Psyr_1925 at 2198.579 kb on - strand, within Psyr_1925 at 2198.607 kb on + strand, within Psyr_1925 at 2198.646 kb on - strand, within Psyr_1925 at 2198.758 kb on + strand, within Psyr_1925 at 2198.759 kb on - strand, within Psyr_1925 at 2198.867 kb on + strand, within Psyr_1925 at 2198.867 kb on + strand, within Psyr_1925 at 2198.867 kb on + strand, within Psyr_1925 at 2198.867 kb on + strand, within Psyr_1925 at 2198.868 kb on - strand, within Psyr_1925 at 2198.868 kb on - strand, within Psyr_1925 at 2198.868 kb on - strand, within Psyr_1925 at 2198.869 kb on + strand, within Psyr_1925 at 2198.870 kb on - strand, within Psyr_1925 at 2198.938 kb on + strand, within Psyr_1925 at 2198.939 kb on - strand, within Psyr_1925 at 2199.033 kb on + strand, within Psyr_1925 at 2199.033 kb on + strand, within Psyr_1925 at 2199.033 kb on + strand, within Psyr_1925 at 2199.034 kb on - strand, within Psyr_1925 at 2199.106 kb on + strand, within Psyr_1925 at 2199.298 kb on + strand at 2199.298 kb on + strand at 2199.298 kb on + strand at 2199.299 kb on - strand at 2199.299 kb on - strand at 2199.302 kb on - strand at 2199.302 kb on - strand at 2199.302 kb on - strand at 2199.302 kb on - strand at 2199.334 kb on - strand at 2199.341 kb on + strand at 2199.341 kb on + strand at 2199.341 kb on + strand at 2199.346 kb on + strand at 2199.347 kb on - strand at 2199.384 kb on + strand at 2199.384 kb on + strand at 2199.384 kb on + strand at 2199.403 kb on + strand at 2199.403 kb on + strand at 2199.403 kb on + strand at 2199.404 kb on - strand at 2199.404 kb on - strand at 2199.404 kb on - strand at 2199.460 kb on - strand, within Psyr_1926 at 2199.590 kb on + strand, within Psyr_1926 at 2199.604 kb on - strand, within Psyr_1926 at 2199.631 kb on + strand, within Psyr_1926 at 2199.632 kb on - strand, within Psyr_1926 at 2199.708 kb on - strand, within Psyr_1926 at 2199.708 kb on - strand, within Psyr_1926 at 2199.708 kb on - strand, within Psyr_1926 at 2199.782 kb on + strand, within Psyr_1926 at 2199.817 kb on + strand, within Psyr_1926 at 2199.817 kb on + strand, within Psyr_1926 at 2199.817 kb on + strand, within Psyr_1926 at 2199.817 kb on + strand, within Psyr_1926 at 2199.817 kb on + strand, within Psyr_1926 at 2199.818 kb on - strand, within Psyr_1926 at 2199.818 kb on - strand, within Psyr_1926 at 2199.818 kb on - strand, within Psyr_1926 at 2199.821 kb on + strand, within Psyr_1926 at 2199.821 kb on + strand, within Psyr_1926 at 2199.821 kb on + strand, within Psyr_1926 at 2199.872 kb on + strand, within Psyr_1926 at 2199.873 kb on - strand, within Psyr_1926 at 2199.943 kb on + strand, within Psyr_1926 at 2200.100 kb on + strand at 2200.107 kb on - strand at 2200.107 kb on - strand at 2200.177 kb on + strand, within Psyr_1927 at 2200.177 kb on + strand, within Psyr_1927 at 2200.177 kb on + strand, within Psyr_1927 at 2200.178 kb on - strand, within Psyr_1927 at 2200.178 kb on - strand, within Psyr_1927 at 2200.178 kb on - strand, within Psyr_1927 at 2200.178 kb on - strand, within Psyr_1927 at 2200.268 kb on - strand, within Psyr_1927 at 2200.271 kb on + strand, within Psyr_1927 at 2200.272 kb on - strand, within Psyr_1927 at 2200.272 kb on - strand, within Psyr_1927 at 2200.272 kb on - strand, within Psyr_1927 at 2200.323 kb on - strand, within Psyr_1927 at 2200.323 kb on - strand, within Psyr_1927 at 2200.326 kb on + strand, within Psyr_1927 at 2200.326 kb on + strand, within Psyr_1927 at 2200.327 kb on - strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.341 kb on + strand, within Psyr_1927 at 2200.342 kb on - strand, within Psyr_1927 at 2200.342 kb on - strand, within Psyr_1927 at 2200.342 kb on - strand, within Psyr_1927 at 2200.342 kb on - strand, within Psyr_1927 at 2200.342 kb on - strand, within Psyr_1927 at 2200.343 kb on + strand, within Psyr_1927 at 2200.372 kb on + strand, within Psyr_1927
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,197,185 - Psyr_1923 0.80 +0.2 2,197,226 + Psyr_1923 0.83 +0.3 2,197,279 + Psyr_1923 0.87 -0.6 2,197,331 - -0.9 2,197,381 - -0.8 2,197,383 - +1.4 2,197,386 + -0.9 2,197,386 + -0.1 2,197,386 + -0.3 2,197,386 + +1.0 2,197,386 + +0.2 2,197,386 + +0.9 2,197,387 - +1.4 2,197,387 - +0.3 2,197,461 + -0.3 2,197,461 + -1.2 2,197,469 + +2.0 2,197,469 + -0.2 2,197,470 - -1.2 2,197,526 - Psyr_1924 0.18 +2.4 2,197,526 - Psyr_1924 0.18 +2.0 2,197,526 - Psyr_1924 0.18 -0.4 2,197,526 - Psyr_1924 0.18 -1.6 2,197,526 - Psyr_1924 0.18 +4.7 2,197,539 - Psyr_1924 0.20 -0.4 2,197,600 + Psyr_1924 0.29 +1.0 2,197,800 + Psyr_1924 0.59 -1.1 2,197,855 + Psyr_1924 0.68 +0.4 2,197,884 + Psyr_1924 0.72 +0.7 2,197,885 - Psyr_1924 0.72 +0.5 2,197,902 - Psyr_1924 0.75 +0.6 2,197,977 + Psyr_1924 0.86 +0.5 2,197,978 - Psyr_1924 0.86 +1.1 2,197,978 - Psyr_1924 0.86 -0.7 2,198,047 + -1.2 2,198,047 + +0.1 2,198,047 + +2.8 2,198,047 + -3.3 2,198,048 - +0.9 2,198,048 - +0.6 2,198,048 - -0.9 2,198,048 - +0.1 2,198,066 - +0.1 2,198,070 + +0.5 2,198,118 + +0.3 2,198,118 + +2.5 2,198,119 - +0.4 2,198,119 - +2.2 2,198,253 + Psyr_1925 0.12 +0.3 2,198,253 + Psyr_1925 0.12 -2.0 2,198,253 + Psyr_1925 0.12 +1.2 2,198,295 + Psyr_1925 0.15 -0.3 2,198,296 - Psyr_1925 0.16 -0.2 2,198,296 - Psyr_1925 0.16 +2.4 2,198,296 - Psyr_1925 0.16 +1.4 2,198,297 + Psyr_1925 0.16 -0.5 2,198,297 + Psyr_1925 0.16 -0.4 2,198,298 - Psyr_1925 0.16 +0.4 2,198,378 + Psyr_1925 0.22 +0.1 2,198,404 - Psyr_1925 0.24 +0.8 2,198,404 - Psyr_1925 0.24 +0.0 2,198,404 - Psyr_1925 0.24 +0.4 2,198,404 - Psyr_1925 0.24 -0.6 2,198,579 - Psyr_1925 0.38 +1.1 2,198,579 - Psyr_1925 0.38 -0.0 2,198,607 + Psyr_1925 0.40 +0.9 2,198,646 - Psyr_1925 0.43 +1.1 2,198,758 + Psyr_1925 0.52 -0.9 2,198,759 - Psyr_1925 0.52 -0.1 2,198,867 + Psyr_1925 0.60 +0.0 2,198,867 + Psyr_1925 0.60 -1.1 2,198,867 + Psyr_1925 0.60 -1.9 2,198,867 + Psyr_1925 0.60 +0.5 2,198,868 - Psyr_1925 0.60 -0.3 2,198,868 - Psyr_1925 0.60 +0.1 2,198,868 - Psyr_1925 0.60 +0.1 2,198,869 + Psyr_1925 0.60 -0.4 2,198,870 - Psyr_1925 0.60 -0.8 2,198,938 + Psyr_1925 0.66 -0.0 2,198,939 - Psyr_1925 0.66 +1.1 2,199,033 + Psyr_1925 0.73 +1.8 2,199,033 + Psyr_1925 0.73 -0.7 2,199,033 + Psyr_1925 0.73 +0.9 2,199,034 - Psyr_1925 0.73 -0.8 2,199,106 + Psyr_1925 0.79 +2.4 2,199,298 + -1.9 2,199,298 + +1.7 2,199,298 + +1.7 2,199,299 - +0.2 2,199,299 - +0.3 2,199,302 - -0.4 2,199,302 - -1.9 2,199,302 - -0.2 2,199,302 - -0.7 2,199,334 - -0.0 2,199,341 + +0.7 2,199,341 + -3.3 2,199,341 + +1.4 2,199,346 + -0.7 2,199,347 - +0.1 2,199,384 + -0.0 2,199,384 + +0.7 2,199,384 + -0.6 2,199,403 + -0.1 2,199,403 + -1.0 2,199,403 + -1.3 2,199,404 - -1.1 2,199,404 - +2.8 2,199,404 - -1.6 2,199,460 - Psyr_1926 0.12 -1.5 2,199,590 + Psyr_1926 0.30 +0.3 2,199,604 - Psyr_1926 0.32 -1.4 2,199,631 + Psyr_1926 0.36 -2.9 2,199,632 - Psyr_1926 0.36 -1.2 2,199,708 - Psyr_1926 0.47 -1.7 2,199,708 - Psyr_1926 0.47 +1.4 2,199,708 - Psyr_1926 0.47 +0.4 2,199,782 + Psyr_1926 0.57 -2.1 2,199,817 + Psyr_1926 0.62 -1.3 2,199,817 + Psyr_1926 0.62 -1.0 2,199,817 + Psyr_1926 0.62 +1.1 2,199,817 + Psyr_1926 0.62 +0.5 2,199,817 + Psyr_1926 0.62 -0.1 2,199,818 - Psyr_1926 0.62 +1.7 2,199,818 - Psyr_1926 0.62 -2.0 2,199,818 - Psyr_1926 0.62 -3.1 2,199,821 + Psyr_1926 0.62 -1.3 2,199,821 + Psyr_1926 0.62 +0.0 2,199,821 + Psyr_1926 0.62 +1.2 2,199,872 + Psyr_1926 0.70 -1.0 2,199,873 - Psyr_1926 0.70 -1.3 2,199,943 + Psyr_1926 0.80 -1.3 2,200,100 + +0.3 2,200,107 - -0.9 2,200,107 - +2.6 2,200,177 + Psyr_1927 0.18 +0.2 2,200,177 + Psyr_1927 0.18 -2.4 2,200,177 + Psyr_1927 0.18 +0.5 2,200,178 - Psyr_1927 0.19 +1.2 2,200,178 - Psyr_1927 0.19 -1.8 2,200,178 - Psyr_1927 0.19 -0.9 2,200,178 - Psyr_1927 0.19 -0.3 2,200,268 - Psyr_1927 0.39 +0.4 2,200,271 + Psyr_1927 0.39 -0.0 2,200,272 - Psyr_1927 0.40 +0.6 2,200,272 - Psyr_1927 0.40 +0.0 2,200,272 - Psyr_1927 0.40 +0.8 2,200,323 - Psyr_1927 0.51 +1.6 2,200,323 - Psyr_1927 0.51 -0.5 2,200,326 + Psyr_1927 0.52 -1.5 2,200,326 + Psyr_1927 0.52 -2.1 2,200,327 - Psyr_1927 0.52 -2.4 2,200,341 + Psyr_1927 0.55 -0.5 2,200,341 + Psyr_1927 0.55 -0.2 2,200,341 + Psyr_1927 0.55 -0.4 2,200,341 + Psyr_1927 0.55 +0.3 2,200,341 + Psyr_1927 0.55 -1.6 2,200,341 + Psyr_1927 0.55 -0.5 2,200,341 + Psyr_1927 0.55 +0.7 2,200,341 + Psyr_1927 0.55 -1.2 2,200,341 + Psyr_1927 0.55 +0.0 2,200,341 + Psyr_1927 0.55 +0.2 2,200,341 + Psyr_1927 0.55 -1.3 2,200,341 + Psyr_1927 0.55 +0.9 2,200,342 - Psyr_1927 0.55 +0.7 2,200,342 - Psyr_1927 0.55 +0.6 2,200,342 - Psyr_1927 0.55 +2.6 2,200,342 - Psyr_1927 0.55 +1.8 2,200,342 - Psyr_1927 0.55 +0.1 2,200,343 + Psyr_1927 0.55 -1.1 2,200,372 + Psyr_1927 0.62 +0.5
Or see this region's nucleotide sequence