Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1683 and Psyr_1684 are separated by 371 nucleotides Psyr_1684 and Psyr_1685 are separated by 141 nucleotides
Psyr_1683: Psyr_1683 - conserved hypothetical protein, at 1,891,318 to 1,892,301
_1683
Psyr_1684: Psyr_1684 - GGDEF domain protein, at 1,892,673 to 1,893,737
_1684
Psyr_1685: Psyr_1685 - NAD(+) kinase, at 1,893,879 to 1,894,769
_1685
Position (kb)
1892
1893
1894 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1891.889 kb on + strand, within Psyr_1683 at 1892.000 kb on - strand, within Psyr_1683 at 1892.293 kb on + strand at 1892.356 kb on + strand at 1892.356 kb on + strand at 1892.356 kb on + strand at 1892.356 kb on + strand at 1892.357 kb on - strand at 1892.357 kb on - strand at 1892.357 kb on - strand at 1892.398 kb on + strand at 1892.399 kb on - strand at 1892.429 kb on - strand at 1892.448 kb on - strand at 1892.490 kb on + strand at 1892.491 kb on - strand at 1892.491 kb on - strand at 1892.492 kb on + strand at 1892.492 kb on + strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.493 kb on - strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.611 kb on + strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.612 kb on - strand at 1892.613 kb on + strand at 1892.613 kb on + strand at 1892.613 kb on + strand at 1892.614 kb on - strand at 1892.614 kb on - strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.615 kb on + strand at 1892.616 kb on - strand at 1892.616 kb on - strand at 1892.616 kb on - strand at 1892.616 kb on - strand at 1892.672 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.738 kb on + strand at 1892.739 kb on - strand at 1892.739 kb on - strand at 1892.879 kb on + strand, within Psyr_1684 at 1892.879 kb on + strand, within Psyr_1684 at 1892.879 kb on + strand, within Psyr_1684 at 1892.879 kb on + strand, within Psyr_1684 at 1892.879 kb on + strand, within Psyr_1684 at 1892.879 kb on + strand, within Psyr_1684 at 1892.880 kb on - strand, within Psyr_1684 at 1892.880 kb on - strand, within Psyr_1684 at 1892.880 kb on - strand, within Psyr_1684 at 1892.880 kb on - strand, within Psyr_1684 at 1892.880 kb on - strand, within Psyr_1684 at 1892.935 kb on + strand, within Psyr_1684 at 1892.935 kb on + strand, within Psyr_1684 at 1892.935 kb on + strand, within Psyr_1684 at 1892.936 kb on - strand, within Psyr_1684 at 1892.936 kb on - strand, within Psyr_1684 at 1892.985 kb on - strand, within Psyr_1684 at 1893.012 kb on + strand, within Psyr_1684 at 1893.012 kb on + strand, within Psyr_1684 at 1893.012 kb on + strand, within Psyr_1684 at 1893.012 kb on + strand, within Psyr_1684 at 1893.013 kb on - strand, within Psyr_1684 at 1893.013 kb on - strand, within Psyr_1684 at 1893.013 kb on - strand, within Psyr_1684 at 1893.013 kb on - strand, within Psyr_1684 at 1893.024 kb on + strand, within Psyr_1684 at 1893.053 kb on + strand, within Psyr_1684 at 1893.053 kb on + strand, within Psyr_1684 at 1893.054 kb on - strand, within Psyr_1684 at 1893.054 kb on - strand, within Psyr_1684 at 1893.054 kb on - strand, within Psyr_1684 at 1893.155 kb on + strand, within Psyr_1684 at 1893.155 kb on + strand, within Psyr_1684 at 1893.155 kb on + strand, within Psyr_1684 at 1893.156 kb on - strand, within Psyr_1684 at 1893.156 kb on - strand, within Psyr_1684 at 1893.156 kb on - strand, within Psyr_1684 at 1893.159 kb on + strand, within Psyr_1684 at 1893.159 kb on + strand, within Psyr_1684 at 1893.159 kb on + strand, within Psyr_1684 at 1893.159 kb on + strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.160 kb on - strand, within Psyr_1684 at 1893.493 kb on - strand, within Psyr_1684 at 1893.624 kb on + strand, within Psyr_1684 at 1893.624 kb on + strand, within Psyr_1684 at 1893.624 kb on + strand, within Psyr_1684 at 1893.624 kb on + strand, within Psyr_1684 at 1893.625 kb on - strand, within Psyr_1684 at 1893.625 kb on - strand, within Psyr_1684 at 1893.625 kb on - strand, within Psyr_1684 at 1893.625 kb on - strand, within Psyr_1684 at 1893.676 kb on + strand at 1893.676 kb on + strand at 1893.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 1,891,889 + Psyr_1683 0.58 +2.4 1,892,000 - Psyr_1683 0.69 -2.6 1,892,293 + +1.1 1,892,356 + +0.3 1,892,356 + +3.0 1,892,356 + +1.4 1,892,356 + +5.0 1,892,357 - -0.2 1,892,357 - -2.9 1,892,357 - -1.5 1,892,398 + +2.3 1,892,399 - -1.1 1,892,429 - -2.7 1,892,448 - -1.8 1,892,490 + +0.7 1,892,491 - -1.0 1,892,491 - -1.2 1,892,492 + +1.2 1,892,492 + +1.6 1,892,493 - +0.6 1,892,493 - -0.3 1,892,493 - -0.1 1,892,493 - -1.7 1,892,493 - -2.8 1,892,493 - -2.7 1,892,493 - +2.6 1,892,611 + +1.5 1,892,611 + +1.7 1,892,611 + +1.1 1,892,611 + +2.6 1,892,611 + +1.0 1,892,611 + +1.9 1,892,611 + +1.8 1,892,612 - -1.5 1,892,612 - -1.8 1,892,612 - -0.5 1,892,612 - -2.4 1,892,612 - -0.5 1,892,612 - -0.8 1,892,612 - -2.7 1,892,612 - -0.4 1,892,612 - +1.8 1,892,612 - -1.8 1,892,613 + +1.0 1,892,613 + +2.2 1,892,613 + +4.1 1,892,614 - -1.4 1,892,614 - -2.7 1,892,615 + +2.8 1,892,615 + +2.3 1,892,615 + +6.0 1,892,615 + +1.9 1,892,615 + +1.4 1,892,615 + +1.8 1,892,615 + +1.5 1,892,615 + +3.1 1,892,615 + +1.5 1,892,615 + +2.6 1,892,615 + -0.0 1,892,616 - +3.0 1,892,616 - +0.2 1,892,616 - -0.3 1,892,616 - -1.0 1,892,672 + -1.8 1,892,738 + -2.0 1,892,738 + -0.5 1,892,738 + -0.6 1,892,738 + +1.7 1,892,738 + -1.5 1,892,738 + -3.1 1,892,738 + -0.6 1,892,739 - -1.6 1,892,739 - -0.3 1,892,879 + Psyr_1684 0.19 -0.6 1,892,879 + Psyr_1684 0.19 +0.6 1,892,879 + Psyr_1684 0.19 +0.4 1,892,879 + Psyr_1684 0.19 -2.3 1,892,879 + Psyr_1684 0.19 -1.2 1,892,879 + Psyr_1684 0.19 +1.5 1,892,880 - Psyr_1684 0.19 -1.4 1,892,880 - Psyr_1684 0.19 +0.0 1,892,880 - Psyr_1684 0.19 -3.0 1,892,880 - Psyr_1684 0.19 -0.3 1,892,880 - Psyr_1684 0.19 -1.3 1,892,935 + Psyr_1684 0.25 -1.6 1,892,935 + Psyr_1684 0.25 -1.9 1,892,935 + Psyr_1684 0.25 -1.7 1,892,936 - Psyr_1684 0.25 -1.4 1,892,936 - Psyr_1684 0.25 -1.2 1,892,985 - Psyr_1684 0.29 +1.6 1,893,012 + Psyr_1684 0.32 +0.4 1,893,012 + Psyr_1684 0.32 -1.8 1,893,012 + Psyr_1684 0.32 +1.9 1,893,012 + Psyr_1684 0.32 -1.8 1,893,013 - Psyr_1684 0.32 -1.9 1,893,013 - Psyr_1684 0.32 -2.9 1,893,013 - Psyr_1684 0.32 -0.1 1,893,013 - Psyr_1684 0.32 -0.5 1,893,024 + Psyr_1684 0.33 -2.5 1,893,053 + Psyr_1684 0.36 -1.3 1,893,053 + Psyr_1684 0.36 -1.1 1,893,054 - Psyr_1684 0.36 -2.6 1,893,054 - Psyr_1684 0.36 -0.0 1,893,054 - Psyr_1684 0.36 -0.9 1,893,155 + Psyr_1684 0.45 -2.2 1,893,155 + Psyr_1684 0.45 -2.0 1,893,155 + Psyr_1684 0.45 -2.3 1,893,156 - Psyr_1684 0.45 +1.1 1,893,156 - Psyr_1684 0.45 -0.8 1,893,156 - Psyr_1684 0.45 -3.1 1,893,159 + Psyr_1684 0.46 -0.8 1,893,159 + Psyr_1684 0.46 -1.9 1,893,159 + Psyr_1684 0.46 -1.8 1,893,159 + Psyr_1684 0.46 -0.5 1,893,160 - Psyr_1684 0.46 +2.5 1,893,160 - Psyr_1684 0.46 -2.2 1,893,160 - Psyr_1684 0.46 -2.6 1,893,160 - Psyr_1684 0.46 -2.4 1,893,160 - Psyr_1684 0.46 -0.8 1,893,160 - Psyr_1684 0.46 -3.6 1,893,493 - Psyr_1684 0.77 +1.9 1,893,624 + Psyr_1684 0.89 -0.5 1,893,624 + Psyr_1684 0.89 -0.2 1,893,624 + Psyr_1684 0.89 -3.2 1,893,624 + Psyr_1684 0.89 -3.2 1,893,625 - Psyr_1684 0.89 +0.5 1,893,625 - Psyr_1684 0.89 -1.6 1,893,625 - Psyr_1684 0.89 -0.3 1,893,625 - Psyr_1684 0.89 -0.1 1,893,676 + +2.3 1,893,676 + +0.5 1,893,798 + -0.3
Or see this region's nucleotide sequence