Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_1299

Experiment: library passed through aphids in food, 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_1298 and Psyr_1299 are separated by 112 nucleotidesPsyr_1299 and Psyr_1300 are separated by 63 nucleotides Psyr_1298: Psyr_1298 - exonuclease RecJ, at 1,465,993 to 1,467,708 _1298 Psyr_1299: Psyr_1299 - NADH:flavin oxidoreductase/NADH oxidase, at 1,467,821 to 1,468,927 _1299 Psyr_1300: Psyr_1300 - PAS/PAC sensor hybrid histidine kinase, at 1,468,991 to 1,471,078 _1300 Position (kb) 1467 1468 1469Strain fitness (log2 ratio) -2 -1 0 1 2at 1467.635 kb on - strandat 1467.748 kb on + strandat 1467.749 kb on - strandat 1467.842 kb on + strandat 1467.842 kb on + strandat 1467.842 kb on + strandat 1467.842 kb on + strandat 1467.842 kb on + strandat 1467.842 kb on + strandat 1467.843 kb on - strandat 1467.843 kb on - strandat 1467.843 kb on - strandat 1467.900 kb on - strandat 1468.072 kb on + strand, within Psyr_1299at 1468.073 kb on - strand, within Psyr_1299at 1468.073 kb on - strand, within Psyr_1299at 1468.177 kb on + strand, within Psyr_1299at 1468.178 kb on - strand, within Psyr_1299at 1468.364 kb on + strand, within Psyr_1299at 1468.364 kb on + strand, within Psyr_1299at 1468.364 kb on + strand, within Psyr_1299at 1468.415 kb on + strand, within Psyr_1299at 1468.416 kb on - strand, within Psyr_1299at 1468.416 kb on - strand, within Psyr_1299at 1468.447 kb on + strand, within Psyr_1299at 1468.608 kb on + strand, within Psyr_1299at 1468.608 kb on + strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.609 kb on - strand, within Psyr_1299at 1468.621 kb on + strand, within Psyr_1299at 1469.281 kb on + strand, within Psyr_1300at 1469.283 kb on + strand, within Psyr_1300at 1469.283 kb on + strand, within Psyr_1300at 1469.283 kb on + strand, within Psyr_1300at 1469.283 kb on + strand, within Psyr_1300at 1469.283 kb on + strand, within Psyr_1300at 1469.284 kb on - strand, within Psyr_1300at 1469.284 kb on - strand, within Psyr_1300at 1469.297 kb on + strand, within Psyr_1300at 1469.297 kb on + strand, within Psyr_1300at 1469.298 kb on - strand, within Psyr_1300at 1469.481 kb on + strand, within Psyr_1300at 1469.481 kb on + strand, within Psyr_1300at 1469.482 kb on - strand, within Psyr_1300at 1469.640 kb on + strand, within Psyr_1300at 1469.641 kb on - strand, within Psyr_1300at 1469.846 kb on + strand, within Psyr_1300at 1469.847 kb on - strand, within Psyr_1300at 1469.899 kb on + strand, within Psyr_1300at 1469.900 kb on - strand, within Psyr_1300at 1469.900 kb on - strand, within Psyr_1300

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours
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1,467,635 - -1.8
1,467,748 + +0.5
1,467,749 - -0.4
1,467,842 + -0.0
1,467,842 + +0.3
1,467,842 + -0.2
1,467,842 + +0.4
1,467,842 + +0.2
1,467,842 + -0.2
1,467,843 - +1.3
1,467,843 - -0.0
1,467,843 - -0.7
1,467,900 - -0.1
1,468,072 + Psyr_1299 0.23 -1.3
1,468,073 - Psyr_1299 0.23 +0.5
1,468,073 - Psyr_1299 0.23 +2.0
1,468,177 + Psyr_1299 0.32 -0.2
1,468,178 - Psyr_1299 0.32 -0.5
1,468,364 + Psyr_1299 0.49 +2.3
1,468,364 + Psyr_1299 0.49 +0.1
1,468,364 + Psyr_1299 0.49 +0.6
1,468,415 + Psyr_1299 0.54 +0.6
1,468,416 - Psyr_1299 0.54 +0.5
1,468,416 - Psyr_1299 0.54 -0.5
1,468,447 + Psyr_1299 0.57 +0.4
1,468,608 + Psyr_1299 0.71 -2.2
1,468,608 + Psyr_1299 0.71 +0.3
1,468,609 - Psyr_1299 0.71 -1.5
1,468,609 - Psyr_1299 0.71 +0.2
1,468,609 - Psyr_1299 0.71 -0.1
1,468,609 - Psyr_1299 0.71 +2.0
1,468,609 - Psyr_1299 0.71 -0.1
1,468,609 - Psyr_1299 0.71 +0.9
1,468,609 - Psyr_1299 0.71 +0.6
1,468,609 - Psyr_1299 0.71 -0.1
1,468,621 + Psyr_1299 0.72 -0.1
1,469,281 + Psyr_1300 0.14 -1.8
1,469,283 + Psyr_1300 0.14 +0.3
1,469,283 + Psyr_1300 0.14 +0.9
1,469,283 + Psyr_1300 0.14 -1.1
1,469,283 + Psyr_1300 0.14 +0.9
1,469,283 + Psyr_1300 0.14 -1.6
1,469,284 - Psyr_1300 0.14 -1.3
1,469,284 - Psyr_1300 0.14 -0.5
1,469,297 + Psyr_1300 0.15 -0.4
1,469,297 + Psyr_1300 0.15 -0.0
1,469,298 - Psyr_1300 0.15 +1.1
1,469,481 + Psyr_1300 0.23 -0.7
1,469,481 + Psyr_1300 0.23 +0.2
1,469,482 - Psyr_1300 0.24 +0.0
1,469,640 + Psyr_1300 0.31 -0.8
1,469,641 - Psyr_1300 0.31 -2.0
1,469,846 + Psyr_1300 0.41 -1.0
1,469,847 - Psyr_1300 0.41 -0.3
1,469,899 + Psyr_1300 0.43 -0.3
1,469,900 - Psyr_1300 0.44 -0.9
1,469,900 - Psyr_1300 0.44 -0.0

Or see this region's nucleotide sequence