Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1296 and Psyr_1297 are separated by 142 nucleotides Psyr_1297 and Psyr_1298 are separated by 61 nucleotides Psyr_1298 and Psyr_1299 are separated by 112 nucleotides
Psyr_1296: Psyr_1296 - conserved hypothetical protein, at 1,464,224 to 1,465,102
_1296
Psyr_1297: Psyr_1297 - YaeQ, at 1,465,245 to 1,465,931
_1297
Psyr_1298: Psyr_1298 - exonuclease RecJ, at 1,465,993 to 1,467,708
_1298
Psyr_1299: Psyr_1299 - NADH:flavin oxidoreductase/NADH oxidase, at 1,467,821 to 1,468,927
_1299
Position (kb)
1465
1466
1467
1468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1465.235 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.244 kb on + strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.245 kb on - strand at 1465.248 kb on + strand at 1465.248 kb on + strand at 1465.248 kb on + strand at 1465.248 kb on + strand at 1465.249 kb on - strand at 1465.249 kb on - strand at 1465.376 kb on + strand, within Psyr_1297 at 1465.376 kb on + strand, within Psyr_1297 at 1465.376 kb on + strand, within Psyr_1297 at 1465.377 kb on - strand, within Psyr_1297 at 1465.377 kb on - strand, within Psyr_1297 at 1465.411 kb on - strand, within Psyr_1297 at 1465.411 kb on - strand, within Psyr_1297 at 1465.527 kb on + strand, within Psyr_1297 at 1465.527 kb on + strand, within Psyr_1297 at 1465.528 kb on - strand, within Psyr_1297 at 1465.528 kb on - strand, within Psyr_1297 at 1465.539 kb on + strand, within Psyr_1297 at 1465.539 kb on + strand, within Psyr_1297 at 1465.539 kb on + strand, within Psyr_1297 at 1465.540 kb on - strand, within Psyr_1297 at 1465.540 kb on - strand, within Psyr_1297 at 1465.540 kb on - strand, within Psyr_1297 at 1465.540 kb on - strand, within Psyr_1297 at 1466.069 kb on - strand at 1466.162 kb on + strand at 1466.293 kb on + strand, within Psyr_1298 at 1466.293 kb on + strand, within Psyr_1298 at 1466.294 kb on - strand, within Psyr_1298 at 1466.332 kb on - strand, within Psyr_1298 at 1466.417 kb on + strand, within Psyr_1298 at 1466.527 kb on - strand, within Psyr_1298 at 1466.527 kb on - strand, within Psyr_1298 at 1466.569 kb on + strand, within Psyr_1298 at 1466.569 kb on + strand, within Psyr_1298 at 1466.570 kb on - strand, within Psyr_1298 at 1466.573 kb on + strand, within Psyr_1298 at 1466.573 kb on + strand, within Psyr_1298 at 1466.573 kb on + strand, within Psyr_1298 at 1466.574 kb on - strand, within Psyr_1298 at 1466.574 kb on - strand, within Psyr_1298 at 1467.635 kb on - strand at 1467.748 kb on + strand at 1467.749 kb on - strand at 1467.842 kb on + strand at 1467.842 kb on + strand at 1467.842 kb on + strand at 1467.842 kb on + strand at 1467.842 kb on + strand at 1467.842 kb on + strand at 1467.843 kb on - strand at 1467.843 kb on - strand at 1467.843 kb on - strand at 1467.900 kb on - strand at 1468.072 kb on + strand, within Psyr_1299 at 1468.073 kb on - strand, within Psyr_1299 at 1468.073 kb on - strand, within Psyr_1299 at 1468.177 kb on + strand, within Psyr_1299 at 1468.178 kb on - strand, within Psyr_1299 at 1468.364 kb on + strand, within Psyr_1299 at 1468.364 kb on + strand, within Psyr_1299 at 1468.364 kb on + strand, within Psyr_1299 at 1468.415 kb on + strand, within Psyr_1299 at 1468.416 kb on - strand, within Psyr_1299 at 1468.416 kb on - strand, within Psyr_1299 at 1468.447 kb on + strand, within Psyr_1299 at 1468.608 kb on + strand, within Psyr_1299 at 1468.608 kb on + strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.609 kb on - strand, within Psyr_1299 at 1468.621 kb on + strand, within Psyr_1299
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 1,465,235 + -0.1 1,465,244 + +1.1 1,465,244 + -0.0 1,465,244 + -0.4 1,465,244 + -1.5 1,465,244 + -1.3 1,465,244 + -1.7 1,465,244 + +3.0 1,465,244 + -0.0 1,465,244 + +0.4 1,465,244 + +0.7 1,465,244 + -3.0 1,465,244 + +0.3 1,465,244 + +0.5 1,465,244 + -0.1 1,465,244 + -0.2 1,465,244 + +0.4 1,465,244 + -0.3 1,465,244 + +0.9 1,465,244 + -0.4 1,465,244 + -1.2 1,465,244 + -0.2 1,465,244 + +0.0 1,465,244 + -1.2 1,465,245 - -0.7 1,465,245 - -0.9 1,465,245 - -1.0 1,465,245 - +0.1 1,465,245 - -0.6 1,465,245 - -1.4 1,465,245 - -2.1 1,465,245 - -3.2 1,465,245 - -0.5 1,465,245 - -1.6 1,465,245 - +0.9 1,465,245 - -1.3 1,465,245 - +0.4 1,465,245 - +0.7 1,465,245 - -0.8 1,465,245 - +2.9 1,465,245 - -1.6 1,465,248 + +1.1 1,465,248 + +1.8 1,465,248 + +1.5 1,465,248 + -1.3 1,465,249 - -1.1 1,465,249 - +0.2 1,465,376 + Psyr_1297 0.19 -0.4 1,465,376 + Psyr_1297 0.19 +1.2 1,465,376 + Psyr_1297 0.19 -0.7 1,465,377 - Psyr_1297 0.19 +0.3 1,465,377 - Psyr_1297 0.19 -0.7 1,465,411 - Psyr_1297 0.24 -0.2 1,465,411 - Psyr_1297 0.24 -0.6 1,465,527 + Psyr_1297 0.41 +0.3 1,465,527 + Psyr_1297 0.41 -0.4 1,465,528 - Psyr_1297 0.41 -1.7 1,465,528 - Psyr_1297 0.41 +4.9 1,465,539 + Psyr_1297 0.43 +0.7 1,465,539 + Psyr_1297 0.43 +1.7 1,465,539 + Psyr_1297 0.43 -1.0 1,465,540 - Psyr_1297 0.43 -0.2 1,465,540 - Psyr_1297 0.43 -2.4 1,465,540 - Psyr_1297 0.43 -1.1 1,465,540 - Psyr_1297 0.43 -0.1 1,466,069 - -0.5 1,466,162 + -1.0 1,466,293 + Psyr_1298 0.17 +0.1 1,466,293 + Psyr_1298 0.17 +0.5 1,466,294 - Psyr_1298 0.18 -0.1 1,466,332 - Psyr_1298 0.20 +0.0 1,466,417 + Psyr_1298 0.25 -1.6 1,466,527 - Psyr_1298 0.31 -1.2 1,466,527 - Psyr_1298 0.31 -0.5 1,466,569 + Psyr_1298 0.34 +1.2 1,466,569 + Psyr_1298 0.34 -1.4 1,466,570 - Psyr_1298 0.34 -0.2 1,466,573 + Psyr_1298 0.34 +0.6 1,466,573 + Psyr_1298 0.34 +0.5 1,466,573 + Psyr_1298 0.34 -0.9 1,466,574 - Psyr_1298 0.34 +0.2 1,466,574 - Psyr_1298 0.34 -0.7 1,467,635 - -1.8 1,467,748 + +0.5 1,467,749 - -0.4 1,467,842 + -0.0 1,467,842 + +0.3 1,467,842 + -0.2 1,467,842 + +0.4 1,467,842 + +0.2 1,467,842 + -0.2 1,467,843 - +1.3 1,467,843 - -0.0 1,467,843 - -0.7 1,467,900 - -0.1 1,468,072 + Psyr_1299 0.23 -1.3 1,468,073 - Psyr_1299 0.23 +0.5 1,468,073 - Psyr_1299 0.23 +2.0 1,468,177 + Psyr_1299 0.32 -0.2 1,468,178 - Psyr_1299 0.32 -0.5 1,468,364 + Psyr_1299 0.49 +2.3 1,468,364 + Psyr_1299 0.49 +0.1 1,468,364 + Psyr_1299 0.49 +0.6 1,468,415 + Psyr_1299 0.54 +0.6 1,468,416 - Psyr_1299 0.54 +0.5 1,468,416 - Psyr_1299 0.54 -0.5 1,468,447 + Psyr_1299 0.57 +0.4 1,468,608 + Psyr_1299 0.71 -2.2 1,468,608 + Psyr_1299 0.71 +0.3 1,468,609 - Psyr_1299 0.71 -1.5 1,468,609 - Psyr_1299 0.71 +0.2 1,468,609 - Psyr_1299 0.71 -0.1 1,468,609 - Psyr_1299 0.71 +2.0 1,468,609 - Psyr_1299 0.71 -0.1 1,468,609 - Psyr_1299 0.71 +0.9 1,468,609 - Psyr_1299 0.71 +0.6 1,468,609 - Psyr_1299 0.71 -0.1 1,468,621 + Psyr_1299 0.72 -0.1
Or see this region's nucleotide sequence