Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0729

Experiment: library passed through aphids in food, 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_RNA9 and Psyr_RNA10 are separated by 29 nucleotidesPsyr_RNA10 and Psyr_RNA11 are separated by 5 nucleotidesPsyr_RNA11 and Psyr_RNA12 are separated by 95 nucleotidesPsyr_RNA12 and Psyr_0729 are separated by 326 nucleotidesPsyr_0729 and Psyr_0730 overlap by 59 nucleotidesPsyr_0730 and Psyr_0731 overlap by 4 nucleotides Psyr_RNA9: Psyr_RNA9 - tRNA-Asn, at 835,185 to 835,260 _RNA9 Psyr_RNA10: Psyr_RNA10 - tRNA-Pro, at 835,290 to 835,366 _RNA10 Psyr_RNA11: Psyr_RNA11 - tRNA-Lys, at 835,372 to 835,447 _RNA11 Psyr_RNA12: Psyr_RNA12 - tRNA-Pro, at 835,543 to 835,619 _RNA12 Psyr_0729: Psyr_0729 - CDS, at 835,946 to 837,109 _0729 Psyr_0730: Psyr_0730 - CDS, at 837,051 to 837,347 _0730 Psyr_0731: Psyr_0731 - CDS, at 837,344 to 838,102 _0731 Position (kb) 835 836 837 838Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 834.959 kb on + strandat 834.959 kb on + strandat 834.959 kb on + strandat 834.959 kb on + strandat 834.959 kb on + strandat 835.021 kb on + strandat 835.085 kb on + strandat 835.085 kb on + strandat 835.128 kb on + strandat 835.160 kb on + strandat 835.367 kb on + strandat 835.367 kb on + strandat 835.367 kb on + strandat 835.367 kb on + strandat 835.367 kb on + strandat 835.421 kb on + strand, within Psyr_RNA11at 835.736 kb on + strandat 835.736 kb on + strandat 835.859 kb on + strandat 835.872 kb on + strandat 835.872 kb on + strandat 835.872 kb on + strandat 835.876 kb on + strandat 835.903 kb on + strandat 835.903 kb on + strandat 835.918 kb on + strandat 835.930 kb on + strandat 835.930 kb on + strandat 835.995 kb on - strandat 835.995 kb on - strandat 835.995 kb on - strandat 836.050 kb on + strandat 836.050 kb on + strandat 836.067 kb on - strandat 836.067 kb on - strand, within Psyr_0729at 836.067 kb on - strand, within Psyr_0729at 836.067 kb on - strand, within Psyr_0729at 836.141 kb on + strand, within Psyr_0729at 836.201 kb on + strand, within Psyr_0729at 836.210 kb on - strand, within Psyr_0729at 836.248 kb on + strand, within Psyr_0729at 836.249 kb on - strand, within Psyr_0729at 836.369 kb on - strand, within Psyr_0729at 836.696 kb on + strand, within Psyr_0729at 836.741 kb on - strand, within Psyr_0729at 836.847 kb on - strand, within Psyr_0729at 836.938 kb on + strand, within Psyr_0729at 836.938 kb on + strand, within Psyr_0729at 837.065 kb on + strandat 837.065 kb on + strandat 837.065 kb on + strandat 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.115 kb on + strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.116 kb on - strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.117 kb on + strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.118 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.140 kb on - strand, within Psyr_0730at 837.593 kb on + strand, within Psyr_0731at 837.593 kb on + strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.594 kb on - strand, within Psyr_0731at 837.627 kb on - strand, within Psyr_0731at 837.627 kb on - strand, within Psyr_0731at 837.627 kb on - strand, within Psyr_0731at 838.003 kb on + strand, within Psyr_0731at 838.037 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours
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834,959 + -0.9
834,959 + +0.7
834,959 + -1.7
834,959 + +0.1
834,959 + -0.1
835,021 + +1.3
835,085 + +2.1
835,085 + +1.0
835,128 + +0.3
835,160 + +0.7
835,367 + -0.1
835,367 + -0.3
835,367 + -1.1
835,367 + -0.8
835,367 + -0.8
835,421 + Psyr_RNA11 0.64 +1.3
835,736 + -0.2
835,736 + +1.3
835,859 + -0.1
835,872 + +2.6
835,872 + +2.1
835,872 + -0.5
835,876 + +2.6
835,903 + +0.1
835,903 + +0.8
835,918 + -1.2
835,930 + -0.2
835,930 + +0.3
835,995 - -0.1
835,995 - -0.3
835,995 - -0.6
836,050 + -1.2
836,050 + +0.5
836,067 - -0.1
836,067 - Psyr_0729 0.10 -0.6
836,067 - Psyr_0729 0.10 -0.7
836,067 - Psyr_0729 0.10 -1.5
836,141 + Psyr_0729 0.17 +0.2
836,201 + Psyr_0729 0.22 -1.3
836,210 - Psyr_0729 0.23 -0.5
836,248 + Psyr_0729 0.26 +0.6
836,249 - Psyr_0729 0.26 -1.6
836,369 - Psyr_0729 0.36 -1.1
836,696 + Psyr_0729 0.64 -0.8
836,741 - Psyr_0729 0.68 -0.6
836,847 - Psyr_0729 0.77 +0.3
836,938 + Psyr_0729 0.85 -0.6
836,938 + Psyr_0729 0.85 +1.2
837,065 + -1.1
837,065 + +2.8
837,065 + +1.3
837,115 + Psyr_0730 0.22 +4.7
837,115 + Psyr_0730 0.22 -1.3
837,115 + Psyr_0730 0.22 +1.4
837,115 + Psyr_0730 0.22 +1.5
837,115 + Psyr_0730 0.22 -0.5
837,115 + Psyr_0730 0.22 +0.6
837,115 + Psyr_0730 0.22 +0.5
837,115 + Psyr_0730 0.22 +1.4
837,115 + Psyr_0730 0.22 +0.9
837,115 + Psyr_0730 0.22 -0.1
837,115 + Psyr_0730 0.22 -0.8
837,115 + Psyr_0730 0.22 +0.4
837,115 + Psyr_0730 0.22 +0.7
837,116 - Psyr_0730 0.22 -0.1
837,116 - Psyr_0730 0.22 -1.4
837,116 - Psyr_0730 0.22 -0.5
837,116 - Psyr_0730 0.22 -0.5
837,116 - Psyr_0730 0.22 -0.5
837,116 - Psyr_0730 0.22 -1.1
837,116 - Psyr_0730 0.22 +0.9
837,116 - Psyr_0730 0.22 +0.7
837,116 - Psyr_0730 0.22 -0.1
837,116 - Psyr_0730 0.22 +0.8
837,116 - Psyr_0730 0.22 -0.5
837,116 - Psyr_0730 0.22 +0.9
837,116 - Psyr_0730 0.22 +0.1
837,116 - Psyr_0730 0.22 -0.6
837,116 - Psyr_0730 0.22 -0.2
837,116 - Psyr_0730 0.22 -0.3
837,116 - Psyr_0730 0.22 -0.8
837,117 + Psyr_0730 0.22 -0.9
837,117 + Psyr_0730 0.22 -0.8
837,117 + Psyr_0730 0.22 -0.4
837,117 + Psyr_0730 0.22 +0.3
837,117 + Psyr_0730 0.22 -0.4
837,118 - Psyr_0730 0.23 -2.1
837,118 - Psyr_0730 0.23 -1.3
837,118 - Psyr_0730 0.23 -2.1
837,118 - Psyr_0730 0.23 -1.1
837,118 - Psyr_0730 0.23 -0.7
837,118 - Psyr_0730 0.23 +0.9
837,118 - Psyr_0730 0.23 -0.4
837,118 - Psyr_0730 0.23 +2.3
837,118 - Psyr_0730 0.23 +2.0
837,118 - Psyr_0730 0.23 -1.3
837,118 - Psyr_0730 0.23 -1.1
837,118 - Psyr_0730 0.23 +0.9
837,140 - Psyr_0730 0.30 -0.1
837,140 - Psyr_0730 0.30 -0.1
837,140 - Psyr_0730 0.30 +2.3
837,140 - Psyr_0730 0.30 +0.2
837,140 - Psyr_0730 0.30 -1.0
837,593 + Psyr_0731 0.33 +1.3
837,593 + Psyr_0731 0.33 +0.2
837,594 - Psyr_0731 0.33 -0.8
837,594 - Psyr_0731 0.33 -0.2
837,594 - Psyr_0731 0.33 -1.0
837,594 - Psyr_0731 0.33 +0.4
837,594 - Psyr_0731 0.33 -0.8
837,594 - Psyr_0731 0.33 -0.4
837,594 - Psyr_0731 0.33 +2.1
837,627 - Psyr_0731 0.37 -2.4
837,627 - Psyr_0731 0.37 -0.6
837,627 - Psyr_0731 0.37 -1.4
838,003 + Psyr_0731 0.87 -0.2
838,037 - +0.3

Or see this region's nucleotide sequence