Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0477

Experiment: library passed through aphids in food, 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0475 and Psyr_0476 are separated by 25 nucleotidesPsyr_0476 and Psyr_0477 are separated by 21 nucleotidesPsyr_0477 and Psyr_0478 are separated by 55 nucleotides Psyr_0475: Psyr_0475 - Protein of unknown function YGGT, at 514,265 to 514,855 _0475 Psyr_0476: Psyr_0476 - pyrroline-5-carboxylate reductase, at 514,881 to 515,699 _0476 Psyr_0477: Psyr_0477 - Protein of unknown function UPF0001, at 515,721 to 516,407 _0477 Psyr_0478: Psyr_0478 - pilus retraction ATPase PilT, at 516,463 to 517,497 _0478 Position (kb) 515 516 517Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 514.746 kb on + strand, within Psyr_0475at 514.746 kb on + strand, within Psyr_0475at 514.746 kb on + strand, within Psyr_0475at 514.746 kb on + strand, within Psyr_0475at 514.746 kb on + strand, within Psyr_0475at 514.746 kb on + strand, within Psyr_0475at 514.787 kb on + strand, within Psyr_0475at 514.787 kb on + strand, within Psyr_0475at 514.787 kb on + strand, within Psyr_0475at 514.788 kb on - strand, within Psyr_0475at 514.800 kb on + strandat 514.828 kb on - strandat 514.828 kb on - strandat 514.828 kb on - strandat 514.828 kb on - strandat 514.828 kb on - strandat 515.167 kb on + strand, within Psyr_0476at 515.496 kb on + strand, within Psyr_0476at 515.734 kb on + strandat 515.996 kb on + strand, within Psyr_0477at 516.117 kb on + strand, within Psyr_0477at 516.117 kb on + strand, within Psyr_0477at 516.118 kb on - strand, within Psyr_0477at 516.236 kb on - strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.268 kb on + strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.269 kb on - strand, within Psyr_0477at 516.302 kb on + strand, within Psyr_0477at 516.444 kb on + strandat 516.444 kb on + strandat 516.444 kb on + strandat 516.444 kb on + strandat 516.444 kb on + strandat 516.444 kb on + strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.445 kb on - strandat 516.462 kb on + strandat 516.463 kb on - strandat 516.463 kb on - strandat 516.469 kb on - strandat 516.570 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.616 kb on + strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.617 kb on - strand, within Psyr_0478at 516.817 kb on - strand, within Psyr_0478at 516.898 kb on + strand, within Psyr_0478at 516.899 kb on - strand, within Psyr_0478at 516.899 kb on - strand, within Psyr_0478at 516.906 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.912 kb on + strand, within Psyr_0478at 516.913 kb on - strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.924 kb on + strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 516.925 kb on - strand, within Psyr_0478at 517.133 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.366 kb on + strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478at 517.367 kb on - strand, within Psyr_0478

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours
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514,746 + Psyr_0475 0.81 -1.7
514,746 + Psyr_0475 0.81 +2.4
514,746 + Psyr_0475 0.81 +0.8
514,746 + Psyr_0475 0.81 +2.4
514,746 + Psyr_0475 0.81 +2.4
514,746 + Psyr_0475 0.81 +2.4
514,787 + Psyr_0475 0.88 +2.4
514,787 + Psyr_0475 0.88 +1.2
514,787 + Psyr_0475 0.88 +2.4
514,788 - Psyr_0475 0.88 +1.4
514,800 + -2.1
514,828 - +0.2
514,828 - -2.3
514,828 - +2.4
514,828 - +0.1
514,828 - -1.4
515,167 + Psyr_0476 0.35 -0.0
515,496 + Psyr_0476 0.75 -0.2
515,734 + +1.3
515,996 + Psyr_0477 0.40 +1.8
516,117 + Psyr_0477 0.58 -0.7
516,117 + Psyr_0477 0.58 +0.5
516,118 - Psyr_0477 0.58 -3.1
516,236 - Psyr_0477 0.75 -0.4
516,268 + Psyr_0477 0.80 -0.7
516,268 + Psyr_0477 0.80 -0.2
516,268 + Psyr_0477 0.80 -1.7
516,268 + Psyr_0477 0.80 -1.0
516,268 + Psyr_0477 0.80 -2.8
516,268 + Psyr_0477 0.80 -2.7
516,268 + Psyr_0477 0.80 -0.9
516,269 - Psyr_0477 0.80 -0.5
516,269 - Psyr_0477 0.80 -0.6
516,269 - Psyr_0477 0.80 +1.0
516,269 - Psyr_0477 0.80 -2.0
516,269 - Psyr_0477 0.80 +1.3
516,269 - Psyr_0477 0.80 +0.8
516,269 - Psyr_0477 0.80 -2.1
516,269 - Psyr_0477 0.80 +0.1
516,269 - Psyr_0477 0.80 -1.2
516,269 - Psyr_0477 0.80 -0.7
516,269 - Psyr_0477 0.80 -2.5
516,269 - Psyr_0477 0.80 -0.4
516,302 + Psyr_0477 0.85 -2.1
516,444 + +0.3
516,444 + +1.0
516,444 + +2.3
516,444 + +0.7
516,444 + -0.4
516,444 + -0.4
516,445 - +1.5
516,445 - +0.7
516,445 - +0.6
516,445 - +1.4
516,445 - +2.3
516,445 - -0.9
516,445 - +0.0
516,445 - +1.1
516,445 - +0.2
516,445 - +0.5
516,445 - +1.0
516,462 + +0.6
516,463 - +2.0
516,463 - +2.2
516,469 - +1.7
516,570 + Psyr_0478 0.10 +2.2
516,616 + Psyr_0478 0.15 +2.1
516,616 + Psyr_0478 0.15 +1.8
516,616 + Psyr_0478 0.15 +0.7
516,616 + Psyr_0478 0.15 +0.1
516,617 - Psyr_0478 0.15 +1.9
516,617 - Psyr_0478 0.15 +5.9
516,617 - Psyr_0478 0.15 +1.5
516,617 - Psyr_0478 0.15 +2.2
516,817 - Psyr_0478 0.34 +0.8
516,898 + Psyr_0478 0.42 +2.3
516,899 - Psyr_0478 0.42 +1.4
516,899 - Psyr_0478 0.42 +2.3
516,906 + Psyr_0478 0.43 +2.0
516,912 + Psyr_0478 0.43 +1.8
516,912 + Psyr_0478 0.43 +1.1
516,912 + Psyr_0478 0.43 +1.1
516,912 + Psyr_0478 0.43 +3.1
516,913 - Psyr_0478 0.43 +1.5
516,924 + Psyr_0478 0.45 +2.3
516,924 + Psyr_0478 0.45 +2.4
516,924 + Psyr_0478 0.45 +0.6
516,925 - Psyr_0478 0.45 +1.5
516,925 - Psyr_0478 0.45 +2.1
516,925 - Psyr_0478 0.45 +1.7
516,925 - Psyr_0478 0.45 +2.5
517,133 + Psyr_0478 0.65 +1.9
517,366 + Psyr_0478 0.87 -0.6
517,366 + Psyr_0478 0.87 +1.5
517,366 + Psyr_0478 0.87 +1.8
517,366 + Psyr_0478 0.87 +2.0
517,366 + Psyr_0478 0.87 +2.1
517,367 - Psyr_0478 0.87 +1.4
517,367 - Psyr_0478 0.87 +0.4
517,367 - Psyr_0478 0.87 +4.0
517,367 - Psyr_0478 0.87 +1.9
517,367 - Psyr_0478 0.87 +2.6
517,367 - Psyr_0478 0.87 +3.2

Or see this region's nucleotide sequence