Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0398 and Psyr_0399 are separated by 169 nucleotides Psyr_0399 and Psyr_0400 are separated by 13 nucleotides Psyr_0400 and Psyr_0401 are separated by 181 nucleotides
Psyr_0398: Psyr_0398 - replication restart DNA helicase PriA, at 425,511 to 427,730
_0398
Psyr_0399: Psyr_0399 - LSU ribosomal protein L31P, at 427,900 to 428,121
_0399
Psyr_0400: Psyr_0400 - nuclease (SNase-like), at 428,135 to 428,938
_0400
Psyr_0401: Psyr_0401 - Malate dehydrogenase (oxaloacetate decarboxylating) (NADP+), at 429,120 to 430,388
_0401
Position (kb) 428
429 Strain fitness (log2 ratio) -2
-1
0
1
2 at 427.751 kb on + strand at 427.752 kb on - strand at 427.752 kb on - strand at 427.752 kb on - strand at 427.776 kb on - strand at 427.825 kb on - strand at 427.861 kb on + strand at 427.861 kb on + strand at 427.862 kb on - strand at 427.862 kb on - strand at 427.896 kb on - strand at 428.030 kb on - strand, within Psyr_0399 at 428.119 kb on + strand at 428.119 kb on + strand at 428.120 kb on - strand at 428.120 kb on - strand at 428.120 kb on - strand at 428.283 kb on + strand, within Psyr_0400 at 428.326 kb on + strand, within Psyr_0400 at 428.327 kb on - strand, within Psyr_0400 at 428.459 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.483 kb on + strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.484 kb on - strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400 at 428.537 kb on + strand, within Psyr_0400 at 428.936 kb on + strand at 428.987 kb on + strand at 428.999 kb on - strand at 428.999 kb on - strand at 429.003 kb on + strand at 429.003 kb on + strand at 429.003 kb on + strand at 429.004 kb on - strand at 429.004 kb on - strand at 429.010 kb on + strand at 429.010 kb on + strand at 429.010 kb on + strand at 429.011 kb on - strand at 429.022 kb on + strand at 429.023 kb on - strand at 429.051 kb on + strand at 429.051 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.057 kb on + strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.058 kb on - strand at 429.070 kb on + strand at 429.098 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.117 kb on + strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.118 kb on - strand at 429.151 kb on - strand at 429.151 kb on - strand at 429.231 kb on + strand at 429.232 kb on - strand at 429.232 kb on - strand at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.288 kb on + strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.289 kb on - strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.294 kb on + strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.295 kb on - strand, within Psyr_0401 at 429.316 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.323 kb on + strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.324 kb on - strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.395 kb on + strand, within Psyr_0401 at 429.491 kb on + strand, within Psyr_0401 at 429.780 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.820 kb on - strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.886 kb on + strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401 at 429.887 kb on - strand, within Psyr_0401
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 427,751 + -1.8 427,752 - +1.2 427,752 - -0.5 427,752 - -0.5 427,776 - -0.6 427,825 - -2.0 427,861 + -1.6 427,861 + +0.1 427,862 - +1.1 427,862 - -0.9 427,896 - +0.1 428,030 - Psyr_0399 0.59 -0.2 428,119 + -1.2 428,119 + -0.8 428,120 - +0.8 428,120 - -0.3 428,120 - -0.2 428,283 + Psyr_0400 0.18 +0.3 428,326 + Psyr_0400 0.24 -0.5 428,327 - Psyr_0400 0.24 -1.0 428,459 + Psyr_0400 0.40 -0.2 428,483 + Psyr_0400 0.43 -1.4 428,483 + Psyr_0400 0.43 -1.2 428,484 - Psyr_0400 0.43 +0.9 428,484 - Psyr_0400 0.43 -0.7 428,484 - Psyr_0400 0.43 +0.1 428,537 + Psyr_0400 0.50 +1.1 428,537 + Psyr_0400 0.50 +1.2 428,936 + -0.7 428,987 + -2.4 428,999 - +0.6 428,999 - -2.1 429,003 + -0.9 429,003 + +0.5 429,003 + -0.9 429,004 - +0.3 429,004 - -0.2 429,010 + -1.4 429,010 + -1.8 429,010 + +0.8 429,011 - +0.1 429,022 + -0.1 429,023 - -1.0 429,051 + -0.9 429,051 + +0.5 429,057 + -1.7 429,057 + +0.3 429,057 + +0.8 429,057 + -1.1 429,057 + +0.0 429,058 - -0.5 429,058 - +0.3 429,058 - -0.4 429,058 - -0.4 429,070 + +1.2 429,098 + -0.1 429,117 + -1.4 429,117 + -0.1 429,117 + -2.0 429,117 + -1.1 429,118 - +0.4 429,118 - -1.8 429,118 - -0.2 429,118 - +0.3 429,118 - -0.4 429,118 - -0.2 429,118 - -0.0 429,118 - -0.7 429,118 - -0.2 429,118 - -1.3 429,151 - -0.2 429,151 - -1.2 429,231 + -1.5 429,232 - -0.1 429,232 - +0.3 429,288 + Psyr_0401 0.13 -0.6 429,288 + Psyr_0401 0.13 -0.2 429,288 + Psyr_0401 0.13 -0.2 429,288 + Psyr_0401 0.13 +0.4 429,289 - Psyr_0401 0.13 -2.5 429,289 - Psyr_0401 0.13 +0.3 429,289 - Psyr_0401 0.13 +0.4 429,294 + Psyr_0401 0.14 -0.6 429,294 + Psyr_0401 0.14 -0.5 429,294 + Psyr_0401 0.14 -1.1 429,294 + Psyr_0401 0.14 -0.4 429,294 + Psyr_0401 0.14 -1.6 429,295 - Psyr_0401 0.14 +0.1 429,295 - Psyr_0401 0.14 -0.4 429,295 - Psyr_0401 0.14 -0.0 429,295 - Psyr_0401 0.14 -0.7 429,295 - Psyr_0401 0.14 +0.1 429,295 - Psyr_0401 0.14 +0.4 429,295 - Psyr_0401 0.14 +0.7 429,295 - Psyr_0401 0.14 -0.4 429,316 + Psyr_0401 0.15 +1.1 429,323 + Psyr_0401 0.16 -0.7 429,323 + Psyr_0401 0.16 +0.1 429,323 + Psyr_0401 0.16 -0.1 429,324 - Psyr_0401 0.16 -0.4 429,324 - Psyr_0401 0.16 -1.4 429,324 - Psyr_0401 0.16 -1.0 429,395 + Psyr_0401 0.22 +0.6 429,395 + Psyr_0401 0.22 +1.7 429,395 + Psyr_0401 0.22 -1.5 429,491 + Psyr_0401 0.29 -1.0 429,780 - Psyr_0401 0.52 +2.3 429,820 - Psyr_0401 0.55 +0.2 429,820 - Psyr_0401 0.55 +1.0 429,886 + Psyr_0401 0.60 -0.7 429,886 + Psyr_0401 0.60 +0.4 429,887 - Psyr_0401 0.60 +0.2 429,887 - Psyr_0401 0.60 +0.3 429,887 - Psyr_0401 0.60 -1.1
Or see this region's nucleotide sequence