Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0313 and Psyr_0314 are separated by 130 nucleotides Psyr_0314 and Psyr_0315 are separated by 6 nucleotides Psyr_0315 and Psyr_0316 are separated by 193 nucleotides Psyr_0316 and Psyr_0317 are separated by 45 nucleotides
Psyr_0313: Psyr_0313 - Zinc-containing alcohol dehydrogenase superfamily, at 332,888 to 333,874
_0313
Psyr_0314: Psyr_0314 - Flagellar basal body-associated protein FliL, at 334,005 to 334,412
_0314
Psyr_0315: Psyr_0315 - Protein of unknown function DUF589, at 334,419 to 334,868
_0315
Psyr_0316: Psyr_0316 - conserved hypothetical protein, at 335,062 to 335,214
_0316
Psyr_0317: Psyr_0317 - 5-formyltetrahydrofolate cyclo-ligase, at 335,260 to 335,871
_0317
Position (kb) 334
335 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 333.215 kb on + strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.216 kb on - strand, within Psyr_0313 at 333.237 kb on + strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.238 kb on - strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.303 kb on + strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.304 kb on - strand, within Psyr_0313 at 333.440 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.567 kb on + strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.568 kb on - strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.723 kb on + strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.724 kb on - strand, within Psyr_0313 at 333.861 kb on - strand at 333.861 kb on - strand at 333.914 kb on + strand at 333.914 kb on + strand at 333.915 kb on - strand at 333.915 kb on - strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.963 kb on + strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.964 kb on - strand at 333.970 kb on + strand at 333.970 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.975 kb on + strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.976 kb on - strand at 333.989 kb on + strand at 333.989 kb on + strand at 333.990 kb on - strand at 333.992 kb on + strand at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.098 kb on + strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.099 kb on - strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.131 kb on + strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.132 kb on - strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.164 kb on + strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.165 kb on - strand, within Psyr_0314 at 334.394 kb on + strand at 334.395 kb on - strand at 334.531 kb on + strand, within Psyr_0315 at 334.661 kb on - strand, within Psyr_0315 at 334.720 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.774 kb on + strand, within Psyr_0315 at 334.775 kb on - strand, within Psyr_0315 at 334.896 kb on - strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.945 kb on + strand at 334.946 kb on - strand at 335.011 kb on + strand at 335.035 kb on + strand at 335.035 kb on + strand at 335.035 kb on + strand at 335.081 kb on + strand, within Psyr_0316 at 335.226 kb on - strand at 335.226 kb on - strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.279 kb on + strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.280 kb on - strand at 335.314 kb on + strand at 335.314 kb on + strand at 335.315 kb on - strand at 335.315 kb on - strand at 335.315 kb on - strand at 335.374 kb on + strand, within Psyr_0317 at 335.411 kb on + strand, within Psyr_0317
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 333,215 + Psyr_0313 0.33 -0.4 333,216 - Psyr_0313 0.33 +0.2 333,216 - Psyr_0313 0.33 +0.8 333,216 - Psyr_0313 0.33 +0.1 333,237 + Psyr_0313 0.35 -0.1 333,238 - Psyr_0313 0.35 +0.5 333,238 - Psyr_0313 0.35 -0.1 333,238 - Psyr_0313 0.35 -1.2 333,238 - Psyr_0313 0.35 +0.3 333,303 + Psyr_0313 0.42 +0.8 333,303 + Psyr_0313 0.42 -0.4 333,303 + Psyr_0313 0.42 +1.5 333,303 + Psyr_0313 0.42 +2.1 333,303 + Psyr_0313 0.42 +1.5 333,304 - Psyr_0313 0.42 -2.1 333,304 - Psyr_0313 0.42 -1.1 333,304 - Psyr_0313 0.42 -0.1 333,304 - Psyr_0313 0.42 +0.0 333,440 + Psyr_0313 0.56 -0.1 333,567 + Psyr_0313 0.69 -0.7 333,567 + Psyr_0313 0.69 -0.7 333,568 - Psyr_0313 0.69 -0.5 333,568 - Psyr_0313 0.69 -0.0 333,568 - Psyr_0313 0.69 +2.7 333,723 + Psyr_0313 0.85 -0.6 333,723 + Psyr_0313 0.85 +0.3 333,724 - Psyr_0313 0.85 -1.2 333,724 - Psyr_0313 0.85 +0.0 333,724 - Psyr_0313 0.85 +0.3 333,724 - Psyr_0313 0.85 -0.2 333,861 - -0.6 333,861 - +1.0 333,914 + +0.5 333,914 + -1.0 333,915 - -1.1 333,915 - -1.2 333,963 + -0.6 333,963 + +1.2 333,963 + -0.3 333,963 + -0.6 333,963 + -0.4 333,963 + -0.8 333,963 + -0.3 333,963 + +1.0 333,963 + +0.2 333,963 + +1.0 333,963 + -0.5 333,963 + +0.9 333,963 + -1.9 333,963 + +0.8 333,964 - +0.6 333,964 - -1.3 333,964 - -1.7 333,964 - +0.4 333,964 - +0.4 333,964 - -1.0 333,964 - +0.3 333,964 - -2.4 333,964 - +1.5 333,964 - -1.6 333,964 - -0.4 333,964 - +1.1 333,964 - -1.7 333,964 - +0.2 333,964 - +0.1 333,964 - -0.6 333,964 - -0.3 333,964 - -0.9 333,964 - +0.9 333,964 - -0.1 333,964 - -0.1 333,964 - +3.3 333,964 - -0.3 333,964 - -1.3 333,964 - +1.3 333,970 + +0.5 333,970 + -0.5 333,975 + -0.6 333,975 + -0.3 333,975 + +1.9 333,975 + -0.5 333,975 + -0.7 333,975 + +0.4 333,975 + +2.9 333,975 + +0.5 333,975 + +2.0 333,975 + -0.2 333,975 + -2.2 333,975 + +0.3 333,976 - -1.2 333,976 - +1.2 333,976 - -0.2 333,976 - -0.3 333,976 - +0.1 333,989 + +1.2 333,989 + -0.7 333,990 - +0.1 333,992 + +0.4 334,098 + Psyr_0314 0.23 -1.1 334,098 + Psyr_0314 0.23 -0.4 334,098 + Psyr_0314 0.23 -2.3 334,098 + Psyr_0314 0.23 -0.8 334,098 + Psyr_0314 0.23 -2.9 334,099 - Psyr_0314 0.23 -1.5 334,099 - Psyr_0314 0.23 -1.6 334,099 - Psyr_0314 0.23 -0.5 334,099 - Psyr_0314 0.23 -2.8 334,131 + Psyr_0314 0.31 -1.6 334,131 + Psyr_0314 0.31 -0.7 334,131 + Psyr_0314 0.31 +0.3 334,131 + Psyr_0314 0.31 -1.1 334,132 - Psyr_0314 0.31 -0.7 334,132 - Psyr_0314 0.31 -1.4 334,132 - Psyr_0314 0.31 -0.5 334,132 - Psyr_0314 0.31 -1.5 334,132 - Psyr_0314 0.31 +0.8 334,164 + Psyr_0314 0.39 -2.2 334,164 + Psyr_0314 0.39 -1.2 334,164 + Psyr_0314 0.39 -2.6 334,164 + Psyr_0314 0.39 -0.2 334,164 + Psyr_0314 0.39 +0.6 334,165 - Psyr_0314 0.39 -0.1 334,165 - Psyr_0314 0.39 -2.0 334,165 - Psyr_0314 0.39 -1.0 334,165 - Psyr_0314 0.39 -0.7 334,394 + +0.4 334,395 - +0.1 334,531 + Psyr_0315 0.25 +0.0 334,661 - Psyr_0315 0.54 -0.5 334,720 + Psyr_0315 0.67 +0.2 334,774 + Psyr_0315 0.79 -0.7 334,774 + Psyr_0315 0.79 -0.6 334,774 + Psyr_0315 0.79 -0.7 334,774 + Psyr_0315 0.79 -1.6 334,775 - Psyr_0315 0.79 +0.5 334,896 - +0.0 334,945 + -0.7 334,945 + +0.7 334,945 + +1.8 334,945 + -0.5 334,946 - -0.5 335,011 + +1.5 335,035 + -2.1 335,035 + +0.2 335,035 + +0.0 335,081 + Psyr_0316 0.12 +0.2 335,226 - -0.3 335,226 - +0.7 335,279 + -1.3 335,279 + +0.2 335,279 + -1.5 335,279 + +0.6 335,279 + +0.8 335,279 + -3.3 335,279 + +0.2 335,279 + +2.4 335,280 - -2.1 335,280 - +0.2 335,280 - -0.6 335,280 - +2.3 335,280 - +0.7 335,280 - -0.7 335,280 - +0.8 335,280 - -0.4 335,280 - +0.1 335,314 + +1.1 335,314 + -1.7 335,315 - +0.8 335,315 - -1.2 335,315 - -0.3 335,374 + Psyr_0317 0.19 -1.1 335,411 + Psyr_0317 0.25 +0.3
Or see this region's nucleotide sequence