Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5595 and Pf6N2E2_5596 are separated by 37 nucleotides Pf6N2E2_5596 and Pf6N2E2_5597 overlap by 4 nucleotides Pf6N2E2_5597 and Pf6N2E2_5598 overlap by 14 nucleotides
Pf6N2E2_5595 - Electron transport complex protein RnfE, at 6,411,344 to 6,411,832
_5595
Pf6N2E2_5596 - Electron transport complex protein RnfG, at 6,411,870 to 6,412,481
_5596
Pf6N2E2_5597 - Electron transport complex protein RnfD, at 6,412,478 to 6,413,464
_5597
Pf6N2E2_5598 - Electron transport complex protein RnfB, at 6,413,451 to 6,414,470
_5598
Position (kb)
6412
6413
6414 Strain fitness (log2 ratio)
-1
0
1
2 at 6411.612 kb on + strand, within Pf6N2E2_5595 at 6411.613 kb on - strand, within Pf6N2E2_5595 at 6411.613 kb on - strand, within Pf6N2E2_5595 at 6411.631 kb on - strand, within Pf6N2E2_5595 at 6411.631 kb on - strand, within Pf6N2E2_5595 at 6411.631 kb on - strand, within Pf6N2E2_5595 at 6411.697 kb on + strand, within Pf6N2E2_5595 at 6411.698 kb on - strand, within Pf6N2E2_5595 at 6411.792 kb on - strand at 6411.871 kb on + strand at 6411.871 kb on + strand at 6411.871 kb on + strand at 6411.871 kb on + strand at 6411.871 kb on + strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.872 kb on - strand at 6411.919 kb on + strand at 6411.920 kb on - strand at 6412.046 kb on + strand, within Pf6N2E2_5596 at 6412.268 kb on + strand, within Pf6N2E2_5596 at 6412.319 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.324 kb on + strand, within Pf6N2E2_5596 at 6412.418 kb on - strand, within Pf6N2E2_5596 at 6412.418 kb on - strand, within Pf6N2E2_5596 at 6412.592 kb on + strand, within Pf6N2E2_5597 at 6412.593 kb on - strand, within Pf6N2E2_5597 at 6412.599 kb on + strand, within Pf6N2E2_5597 at 6412.600 kb on - strand, within Pf6N2E2_5597 at 6412.781 kb on + strand, within Pf6N2E2_5597 at 6413.012 kb on + strand, within Pf6N2E2_5597 at 6413.091 kb on - strand, within Pf6N2E2_5597 at 6413.091 kb on - strand, within Pf6N2E2_5597 at 6413.208 kb on + strand, within Pf6N2E2_5597 at 6413.208 kb on + strand, within Pf6N2E2_5597 at 6413.209 kb on - strand, within Pf6N2E2_5597 at 6413.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 6,411,612 + Pf6N2E2_5595 0.55 +0.1 6,411,613 - Pf6N2E2_5595 0.55 -0.1 6,411,613 - Pf6N2E2_5595 0.55 -0.5 6,411,631 - Pf6N2E2_5595 0.59 +0.6 6,411,631 - Pf6N2E2_5595 0.59 -1.4 6,411,631 - Pf6N2E2_5595 0.59 +0.6 6,411,697 + Pf6N2E2_5595 0.72 -0.3 6,411,698 - Pf6N2E2_5595 0.72 -0.2 6,411,792 - +0.4 6,411,871 + +2.1 6,411,871 + +1.1 6,411,871 + -0.7 6,411,871 + +0.2 6,411,871 + -1.2 6,411,872 - -0.1 6,411,872 - +0.5 6,411,872 - +0.3 6,411,872 - +0.8 6,411,872 - -0.5 6,411,872 - +0.7 6,411,872 - -0.2 6,411,919 + +0.8 6,411,920 - +0.7 6,412,046 + Pf6N2E2_5596 0.29 +0.2 6,412,268 + Pf6N2E2_5596 0.65 +1.2 6,412,319 + Pf6N2E2_5596 0.73 -0.3 6,412,324 + Pf6N2E2_5596 0.74 -0.3 6,412,324 + Pf6N2E2_5596 0.74 -0.2 6,412,324 + Pf6N2E2_5596 0.74 -0.4 6,412,324 + Pf6N2E2_5596 0.74 -0.1 6,412,324 + Pf6N2E2_5596 0.74 +0.8 6,412,324 + Pf6N2E2_5596 0.74 -0.8 6,412,418 - Pf6N2E2_5596 0.90 -1.1 6,412,418 - Pf6N2E2_5596 0.90 +0.3 6,412,592 + Pf6N2E2_5597 0.12 +0.2 6,412,593 - Pf6N2E2_5597 0.12 -0.2 6,412,599 + Pf6N2E2_5597 0.12 -1.0 6,412,600 - Pf6N2E2_5597 0.12 -0.4 6,412,781 + Pf6N2E2_5597 0.31 -1.5 6,413,012 + Pf6N2E2_5597 0.54 -1.7 6,413,091 - Pf6N2E2_5597 0.62 -1.3 6,413,091 - Pf6N2E2_5597 0.62 -0.7 6,413,208 + Pf6N2E2_5597 0.74 +0.0 6,413,208 + Pf6N2E2_5597 0.74 +0.7 6,413,209 - Pf6N2E2_5597 0.74 -0.7 6,413,535 - +0.7
Or see this region's nucleotide sequence