Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4 and Pf6N2E2_5 are separated by 11 nucleotides Pf6N2E2_5 and Pf6N2E2_6 overlap by 4 nucleotides
Pf6N2E2_4 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 14,454 to 20,954
_4
Pf6N2E2_5 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 20,966 to 27,997
_5
Pf6N2E2_6 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 27,994 to 31,221
_6
Position (kb) 20
21
22
23
24
25
26
27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 20.028 kb on + strand, within Pf6N2E2_4 at 20.028 kb on + strand, within Pf6N2E2_4 at 20.029 kb on - strand, within Pf6N2E2_4 at 20.029 kb on - strand, within Pf6N2E2_4 at 20.038 kb on - strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.193 kb on + strand, within Pf6N2E2_4 at 20.194 kb on - strand, within Pf6N2E2_4 at 20.479 kb on - strand at 20.479 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.692 kb on + strand at 20.692 kb on + strand at 20.693 kb on - strand at 21.210 kb on + strand at 21.417 kb on - strand at 21.536 kb on + strand at 21.980 kb on - strand, within Pf6N2E2_5 at 22.020 kb on - strand, within Pf6N2E2_5 at 22.232 kb on + strand, within Pf6N2E2_5 at 22.233 kb on - strand, within Pf6N2E2_5 at 22.233 kb on - strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.443 kb on - strand, within Pf6N2E2_5 at 22.757 kb on + strand, within Pf6N2E2_5 at 22.758 kb on - strand, within Pf6N2E2_5 at 22.871 kb on + strand, within Pf6N2E2_5 at 23.538 kb on - strand, within Pf6N2E2_5 at 23.538 kb on - strand, within Pf6N2E2_5 at 23.801 kb on + strand, within Pf6N2E2_5 at 23.802 kb on - strand, within Pf6N2E2_5 at 23.802 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.170 kb on + strand, within Pf6N2E2_5 at 24.171 kb on - strand, within Pf6N2E2_5 at 24.257 kb on + strand, within Pf6N2E2_5 at 24.584 kb on + strand, within Pf6N2E2_5 at 24.584 kb on + strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.691 kb on + strand, within Pf6N2E2_5 at 24.692 kb on - strand, within Pf6N2E2_5 at 24.698 kb on - strand, within Pf6N2E2_5 at 24.750 kb on - strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.303 kb on - strand, within Pf6N2E2_5 at 25.311 kb on + strand, within Pf6N2E2_5 at 25.311 kb on + strand, within Pf6N2E2_5 at 25.406 kb on + strand, within Pf6N2E2_5 at 25.613 kb on + strand, within Pf6N2E2_5 at 25.718 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.938 kb on - strand, within Pf6N2E2_5 at 26.060 kb on + strand, within Pf6N2E2_5 at 26.061 kb on - strand, within Pf6N2E2_5 at 26.063 kb on + strand, within Pf6N2E2_5 at 26.063 kb on + strand, within Pf6N2E2_5 at 26.151 kb on - strand, within Pf6N2E2_5 at 26.204 kb on + strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.614 kb on - strand, within Pf6N2E2_5 at 26.614 kb on - strand, within Pf6N2E2_5 at 26.681 kb on - strand, within Pf6N2E2_5 at 27.233 kb on + strand, within Pf6N2E2_5 at 27.234 kb on - strand, within Pf6N2E2_5 at 27.323 kb on + strand at 27.323 kb on + strand at 27.324 kb on - strand at 27.324 kb on - strand at 27.383 kb on + strand at 27.656 kb on + strand at 27.675 kb on + strand at 27.676 kb on - strand at 27.728 kb on + strand at 27.728 kb on + strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.731 kb on + strand at 27.732 kb on - strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.786 kb on - strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.911 kb on - strand at 27.911 kb on - strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.915 kb on - strand at 28.170 kb on - strand at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.376 kb on - strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.467 kb on - strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.529 kb on - strand, within Pf6N2E2_6 at 28.586 kb on - strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.859 kb on - strand, within Pf6N2E2_6
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 20,028 + Pf6N2E2_4 0.86 -3.0 20,028 + Pf6N2E2_4 0.86 -2.2 20,029 - Pf6N2E2_4 0.86 +1.0 20,029 - Pf6N2E2_4 0.86 +0.3 20,038 - Pf6N2E2_4 0.86 -0.9 20,139 + Pf6N2E2_4 0.87 -1.0 20,139 + Pf6N2E2_4 0.87 -0.3 20,139 + Pf6N2E2_4 0.87 -0.2 20,140 - Pf6N2E2_4 0.87 +0.3 20,140 - Pf6N2E2_4 0.87 -0.1 20,140 - Pf6N2E2_4 0.87 +0.3 20,140 - Pf6N2E2_4 0.87 +0.1 20,140 - Pf6N2E2_4 0.87 -1.2 20,193 + Pf6N2E2_4 0.88 +0.4 20,194 - Pf6N2E2_4 0.88 -1.5 20,479 - -0.8 20,479 - -2.9 20,581 - -0.8 20,581 - -0.2 20,581 - +0.8 20,581 - -2.5 20,581 - +1.0 20,692 + +0.9 20,692 + +0.9 20,693 - -0.9 21,210 + +0.0 21,417 - +0.3 21,536 + -2.0 21,980 - Pf6N2E2_5 0.14 -1.3 22,020 - Pf6N2E2_5 0.15 -1.0 22,232 + Pf6N2E2_5 0.18 +0.4 22,233 - Pf6N2E2_5 0.18 -0.2 22,233 - Pf6N2E2_5 0.18 -1.2 22,376 + Pf6N2E2_5 0.20 -0.1 22,376 + Pf6N2E2_5 0.20 +1.3 22,376 + Pf6N2E2_5 0.20 -0.2 22,376 + Pf6N2E2_5 0.20 +0.3 22,376 + Pf6N2E2_5 0.20 -0.3 22,377 - Pf6N2E2_5 0.20 +1.0 22,377 - Pf6N2E2_5 0.20 -1.5 22,377 - Pf6N2E2_5 0.20 -0.0 22,377 - Pf6N2E2_5 0.20 -0.5 22,377 - Pf6N2E2_5 0.20 -0.2 22,442 + Pf6N2E2_5 0.21 +0.1 22,442 + Pf6N2E2_5 0.21 +0.2 22,442 + Pf6N2E2_5 0.21 +1.5 22,443 - Pf6N2E2_5 0.21 -0.5 22,757 + Pf6N2E2_5 0.25 -0.6 22,758 - Pf6N2E2_5 0.25 -0.3 22,871 + Pf6N2E2_5 0.27 +0.2 23,538 - Pf6N2E2_5 0.37 -1.7 23,538 - Pf6N2E2_5 0.37 -0.6 23,801 + Pf6N2E2_5 0.40 -0.8 23,802 - Pf6N2E2_5 0.40 -0.1 23,802 - Pf6N2E2_5 0.40 +0.3 23,991 - Pf6N2E2_5 0.43 +0.9 23,991 - Pf6N2E2_5 0.43 -1.4 23,991 - Pf6N2E2_5 0.43 -0.2 24,162 - Pf6N2E2_5 0.45 +0.4 24,162 - Pf6N2E2_5 0.45 +1.1 24,162 - Pf6N2E2_5 0.45 -0.6 24,162 - Pf6N2E2_5 0.45 +0.9 24,170 + Pf6N2E2_5 0.46 +0.1 24,171 - Pf6N2E2_5 0.46 -1.1 24,257 + Pf6N2E2_5 0.47 -0.5 24,584 + Pf6N2E2_5 0.51 +0.2 24,584 + Pf6N2E2_5 0.51 -1.9 24,585 - Pf6N2E2_5 0.51 +1.1 24,585 - Pf6N2E2_5 0.51 -1.6 24,585 - Pf6N2E2_5 0.51 +0.3 24,691 + Pf6N2E2_5 0.53 -0.2 24,692 - Pf6N2E2_5 0.53 +0.3 24,698 - Pf6N2E2_5 0.53 -0.1 24,750 - Pf6N2E2_5 0.54 -0.5 25,136 + Pf6N2E2_5 0.59 +0.5 25,136 + Pf6N2E2_5 0.59 +0.1 25,136 + Pf6N2E2_5 0.59 +0.0 25,137 - Pf6N2E2_5 0.59 -0.8 25,137 - Pf6N2E2_5 0.59 +0.8 25,137 - Pf6N2E2_5 0.59 +0.3 25,303 - Pf6N2E2_5 0.62 +3.6 25,311 + Pf6N2E2_5 0.62 +0.9 25,311 + Pf6N2E2_5 0.62 +0.0 25,406 + Pf6N2E2_5 0.63 +0.9 25,613 + Pf6N2E2_5 0.66 +1.3 25,718 + Pf6N2E2_5 0.68 -0.7 25,937 + Pf6N2E2_5 0.71 +0.1 25,937 + Pf6N2E2_5 0.71 -1.3 25,937 + Pf6N2E2_5 0.71 +0.2 25,937 + Pf6N2E2_5 0.71 -0.9 25,937 + Pf6N2E2_5 0.71 +0.8 25,937 + Pf6N2E2_5 0.71 -0.4 25,937 + Pf6N2E2_5 0.71 -2.2 25,937 + Pf6N2E2_5 0.71 +0.5 25,937 + Pf6N2E2_5 0.71 -0.5 25,937 + Pf6N2E2_5 0.71 +0.6 25,938 - Pf6N2E2_5 0.71 +0.4 26,060 + Pf6N2E2_5 0.72 -0.6 26,061 - Pf6N2E2_5 0.72 +0.8 26,063 + Pf6N2E2_5 0.72 -0.9 26,063 + Pf6N2E2_5 0.72 +1.4 26,151 - Pf6N2E2_5 0.74 -0.5 26,204 + Pf6N2E2_5 0.74 -0.2 26,205 - Pf6N2E2_5 0.75 +1.2 26,205 - Pf6N2E2_5 0.75 -0.3 26,205 - Pf6N2E2_5 0.75 +0.3 26,291 + Pf6N2E2_5 0.76 +0.0 26,291 + Pf6N2E2_5 0.76 -0.2 26,291 + Pf6N2E2_5 0.76 -0.4 26,614 - Pf6N2E2_5 0.80 -0.2 26,614 - Pf6N2E2_5 0.80 -0.5 26,681 - Pf6N2E2_5 0.81 +0.8 27,233 + Pf6N2E2_5 0.89 -0.0 27,234 - Pf6N2E2_5 0.89 -2.9 27,323 + +1.3 27,323 + +0.3 27,324 - +0.8 27,324 - +0.2 27,383 + -0.1 27,656 + -1.3 27,675 + -1.0 27,676 - +0.6 27,728 + -0.4 27,728 + -0.1 27,729 - -1.1 27,729 - +0.5 27,729 - +0.1 27,729 - +0.2 27,729 - -0.1 27,731 + -1.2 27,732 - -1.1 27,785 + -1.9 27,785 + +1.0 27,785 + +0.6 27,786 - -0.6 27,910 + +0.4 27,910 + +0.0 27,910 + -0.4 27,911 - -0.1 27,911 - -0.5 27,914 + -0.4 27,914 + -0.3 27,914 + +0.7 27,914 + -1.2 27,914 + -0.5 27,914 + -1.9 27,914 + +1.0 27,914 + +0.8 27,915 - -0.1 28,170 - -1.4 28,373 + Pf6N2E2_6 0.12 -2.3 28,373 + Pf6N2E2_6 0.12 -2.3 28,373 + Pf6N2E2_6 0.12 +0.7 28,376 - Pf6N2E2_6 0.12 +0.3 28,466 + Pf6N2E2_6 0.15 -1.6 28,466 + Pf6N2E2_6 0.15 -2.2 28,466 + Pf6N2E2_6 0.15 +0.4 28,467 - Pf6N2E2_6 0.15 -0.3 28,477 + Pf6N2E2_6 0.15 -0.2 28,477 + Pf6N2E2_6 0.15 +0.1 28,478 - Pf6N2E2_6 0.15 +1.4 28,478 - Pf6N2E2_6 0.15 +0.4 28,529 - Pf6N2E2_6 0.17 -0.9 28,586 - Pf6N2E2_6 0.18 -0.7 28,858 + Pf6N2E2_6 0.27 +1.0 28,858 + Pf6N2E2_6 0.27 -0.1 28,859 - Pf6N2E2_6 0.27 -0.9
Or see this region's nucleotide sequence