Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_436 and Pf6N2E2_437 are separated by 88 nucleotides Pf6N2E2_437 and Pf6N2E2_438 overlap by 11 nucleotides Pf6N2E2_438 and Pf6N2E2_439 are separated by 301 nucleotides Pf6N2E2_439 and Pf6N2E2_440 are separated by 30 nucleotides
Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085
_436
Pf6N2E2_437 - Alkylated DNA repair protein AlkB, at 503,174 to 503,851
_437
Pf6N2E2_438 - hypothetical protein, at 503,841 to 504,569
_438
Pf6N2E2_439 - FIG00958580: hypothetical protein, at 504,871 to 505,041
_439
Pf6N2E2_440 - FIG00954920: hypothetical protein, at 505,072 to 505,374
_440
Position (kb) 503
504
505 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.984 kb on + strand at 502.991 kb on + strand at 502.991 kb on + strand at 502.992 kb on - strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.093 kb on - strand at 503.116 kb on - strand at 503.116 kb on - strand at 503.417 kb on - strand, within Pf6N2E2_437 at 503.417 kb on - strand, within Pf6N2E2_437 at 503.452 kb on - strand, within Pf6N2E2_437 at 503.581 kb on + strand, within Pf6N2E2_437 at 503.583 kb on + strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.647 kb on + strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.650 kb on + strand, within Pf6N2E2_437 at 503.650 kb on + strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.882 kb on + strand at 504.059 kb on + strand, within Pf6N2E2_438 at 504.122 kb on - strand, within Pf6N2E2_438 at 504.145 kb on + strand, within Pf6N2E2_438 at 504.146 kb on - strand, within Pf6N2E2_438 at 504.146 kb on - strand, within Pf6N2E2_438 at 504.161 kb on - strand, within Pf6N2E2_438 at 504.299 kb on - strand, within Pf6N2E2_438 at 504.355 kb on + strand, within Pf6N2E2_438 at 504.528 kb on + strand at 504.528 kb on + strand at 504.529 kb on - strand at 504.529 kb on - strand at 504.856 kb on + strand at 504.857 kb on - strand at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.011 kb on - strand, within Pf6N2E2_439 at 505.011 kb on - strand, within Pf6N2E2_439 at 505.022 kb on + strand, within Pf6N2E2_439 at 505.022 kb on + strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.269 kb on + strand, within Pf6N2E2_440 at 505.440 kb on + strand at 505.441 kb on - strand at 505.457 kb on - strand at 505.457 kb on - strand at 505.457 kb on - strand at 505.459 kb on + strand at 505.491 kb on - strand at 505.491 kb on - strand at 505.511 kb on + strand at 505.512 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 502,967 + Pf6N2E2_436 0.89 +0.2 502,967 + Pf6N2E2_436 0.89 +0.5 502,967 + Pf6N2E2_436 0.89 -0.4 502,967 + Pf6N2E2_436 0.89 +0.4 502,967 + Pf6N2E2_436 0.89 +0.5 502,967 + Pf6N2E2_436 0.89 -0.9 502,967 + Pf6N2E2_436 0.89 -0.6 502,968 - Pf6N2E2_436 0.89 +0.5 502,968 - Pf6N2E2_436 0.89 -0.5 502,968 - Pf6N2E2_436 0.89 +0.9 502,984 + -2.0 502,991 + -0.7 502,991 + -1.7 502,992 - +0.5 503,092 + +0.2 503,092 + +0.4 503,092 + +0.8 503,092 + -0.4 503,093 - +0.3 503,116 - -0.9 503,116 - +0.3 503,417 - Pf6N2E2_437 0.36 +0.6 503,417 - Pf6N2E2_437 0.36 -0.2 503,452 - Pf6N2E2_437 0.41 -3.3 503,581 + Pf6N2E2_437 0.60 -0.9 503,583 + Pf6N2E2_437 0.60 -0.0 503,584 - Pf6N2E2_437 0.60 +0.6 503,584 - Pf6N2E2_437 0.60 +0.2 503,584 - Pf6N2E2_437 0.60 -0.7 503,647 + Pf6N2E2_437 0.70 +1.0 503,648 - Pf6N2E2_437 0.70 -1.5 503,648 - Pf6N2E2_437 0.70 +0.7 503,648 - Pf6N2E2_437 0.70 +0.3 503,648 - Pf6N2E2_437 0.70 +0.5 503,650 + Pf6N2E2_437 0.70 +0.1 503,650 + Pf6N2E2_437 0.70 -0.4 503,651 - Pf6N2E2_437 0.70 +2.2 503,651 - Pf6N2E2_437 0.70 -1.6 503,651 - Pf6N2E2_437 0.70 -2.1 503,651 - Pf6N2E2_437 0.70 -0.4 503,882 + +0.2 504,059 + Pf6N2E2_438 0.30 +0.4 504,122 - Pf6N2E2_438 0.39 -2.0 504,145 + Pf6N2E2_438 0.42 -0.5 504,146 - Pf6N2E2_438 0.42 -0.7 504,146 - Pf6N2E2_438 0.42 -0.8 504,161 - Pf6N2E2_438 0.44 -0.6 504,299 - Pf6N2E2_438 0.63 -2.1 504,355 + Pf6N2E2_438 0.71 -0.9 504,528 + +1.0 504,528 + -0.4 504,529 - -1.5 504,529 - +0.5 504,856 + -0.1 504,857 - -1.8 505,010 + Pf6N2E2_439 0.81 -2.3 505,010 + Pf6N2E2_439 0.81 -0.0 505,010 + Pf6N2E2_439 0.81 +0.1 505,010 + Pf6N2E2_439 0.81 -0.9 505,011 - Pf6N2E2_439 0.82 +1.0 505,011 - Pf6N2E2_439 0.82 -1.1 505,022 + Pf6N2E2_439 0.88 +0.2 505,022 + Pf6N2E2_439 0.88 -0.3 505,023 - Pf6N2E2_439 0.89 +1.0 505,023 - Pf6N2E2_439 0.89 -0.3 505,023 - Pf6N2E2_439 0.89 -0.1 505,023 - Pf6N2E2_439 0.89 -0.7 505,242 - Pf6N2E2_440 0.56 -2.1 505,242 - Pf6N2E2_440 0.56 +1.7 505,242 - Pf6N2E2_440 0.56 +0.1 505,242 - Pf6N2E2_440 0.56 +0.8 505,269 + Pf6N2E2_440 0.65 +1.4 505,440 + +1.9 505,441 - -0.5 505,457 - -0.2 505,457 - +0.3 505,457 - -1.1 505,459 + +0.3 505,491 - +0.3 505,491 - +0.9 505,511 + +0.1 505,512 - -1.6
Or see this region's nucleotide sequence