Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_332 and Pf6N2E2_333 are separated by 17 nucleotides Pf6N2E2_333 and Pf6N2E2_334 are separated by 167 nucleotides Pf6N2E2_334 and Pf6N2E2_335 are separated by 100 nucleotides Pf6N2E2_335 and Pf6N2E2_336 overlap by 4 nucleotides
Pf6N2E2_332 - putative nucleoside-diphosphate-sugar epimerase, at 399,970 to 400,848
_332
Pf6N2E2_333 - hypothetical protein, at 400,866 to 401,549
_333
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Pf6N2E2_335 - Aldo-keto reductase, at 402,771 to 403,634
_335
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Position (kb) 401
402
403 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.854 kb on + strand at 400.854 kb on + strand at 401.047 kb on + strand, within Pf6N2E2_333 at 401.047 kb on + strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.143 kb on + strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.192 kb on - strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.275 kb on - strand, within Pf6N2E2_333 at 401.438 kb on - strand, within Pf6N2E2_333 at 401.501 kb on - strand at 401.501 kb on - strand at 401.549 kb on + strand at 401.649 kb on + strand at 401.649 kb on + strand at 401.650 kb on - strand at 401.838 kb on + strand, within Pf6N2E2_334 at 401.841 kb on + strand, within Pf6N2E2_334 at 401.910 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.938 kb on - strand, within Pf6N2E2_334 at 401.957 kb on + strand, within Pf6N2E2_334 at 401.958 kb on - strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.151 kb on - strand, within Pf6N2E2_334 at 402.211 kb on + strand, within Pf6N2E2_334 at 402.212 kb on - strand, within Pf6N2E2_334 at 402.337 kb on + strand, within Pf6N2E2_334 at 402.686 kb on - strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.957 kb on + strand, within Pf6N2E2_335 at 403.006 kb on - strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.633 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 400,844 + -0.9 400,844 + -0.0 400,844 + -1.3 400,844 + -1.7 400,844 + -0.3 400,845 - -1.4 400,845 - -0.2 400,845 - +0.3 400,845 - +1.3 400,845 - -0.5 400,854 + +0.3 400,854 + -0.3 401,047 + Pf6N2E2_333 0.26 +0.7 401,047 + Pf6N2E2_333 0.26 -0.2 401,048 - Pf6N2E2_333 0.27 -1.7 401,048 - Pf6N2E2_333 0.27 +0.1 401,048 - Pf6N2E2_333 0.27 +0.9 401,048 - Pf6N2E2_333 0.27 +0.5 401,143 + Pf6N2E2_333 0.40 +0.9 401,144 - Pf6N2E2_333 0.41 -0.7 401,144 - Pf6N2E2_333 0.41 +0.2 401,144 - Pf6N2E2_333 0.41 -0.7 401,191 + Pf6N2E2_333 0.48 -0.1 401,191 + Pf6N2E2_333 0.48 -1.0 401,192 - Pf6N2E2_333 0.48 +0.8 401,274 + Pf6N2E2_333 0.60 +0.2 401,274 + Pf6N2E2_333 0.60 -1.1 401,274 + Pf6N2E2_333 0.60 +0.1 401,275 - Pf6N2E2_333 0.60 -1.3 401,438 - Pf6N2E2_333 0.84 -2.6 401,501 - +0.5 401,501 - +0.4 401,549 + +0.2 401,649 + -0.1 401,649 + +0.7 401,650 - -0.6 401,838 + Pf6N2E2_334 0.13 +1.9 401,841 + Pf6N2E2_334 0.13 -1.4 401,910 + Pf6N2E2_334 0.20 +0.7 401,937 + Pf6N2E2_334 0.23 -1.4 401,937 + Pf6N2E2_334 0.23 -1.4 401,937 + Pf6N2E2_334 0.23 +1.2 401,938 - Pf6N2E2_334 0.23 -2.6 401,957 + Pf6N2E2_334 0.25 -1.6 401,958 - Pf6N2E2_334 0.25 -1.1 402,051 + Pf6N2E2_334 0.35 +0.6 402,051 + Pf6N2E2_334 0.35 -0.2 402,150 + Pf6N2E2_334 0.45 -0.4 402,150 + Pf6N2E2_334 0.45 -2.2 402,151 - Pf6N2E2_334 0.45 +0.5 402,211 + Pf6N2E2_334 0.52 -1.3 402,212 - Pf6N2E2_334 0.52 -2.7 402,337 + Pf6N2E2_334 0.65 -1.3 402,686 - +0.3 402,733 + +0.7 402,733 + +1.3 402,733 + -0.1 402,733 + -0.2 402,733 + -1.1 402,734 - -0.9 402,734 - -0.4 402,734 - +2.2 402,734 - +0.3 402,734 - -0.3 402,734 - -1.8 402,957 + Pf6N2E2_335 0.22 +0.1 403,006 - Pf6N2E2_335 0.27 -0.1 403,332 + Pf6N2E2_335 0.65 -3.0 403,332 + Pf6N2E2_335 0.65 +0.2 403,395 + Pf6N2E2_335 0.72 -0.3 403,395 + Pf6N2E2_335 0.72 +0.6 403,396 - Pf6N2E2_335 0.72 -0.0 403,396 - Pf6N2E2_335 0.72 +0.4 403,396 - Pf6N2E2_335 0.72 -0.4 403,633 - +0.9
Or see this region's nucleotide sequence