Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_185 and Pf6N2E2_186 overlap by 4 nucleotides Pf6N2E2_186 and Pf6N2E2_187 are separated by 10 nucleotides Pf6N2E2_187 and Pf6N2E2_188 are separated by 132 nucleotides
Pf6N2E2_185 - Urea ABC transporter, permease protein UrtC, at 237,965 to 239,152
_185
Pf6N2E2_186 - Urea ABC transporter, ATPase protein UrtD, at 239,149 to 239,907
_186
Pf6N2E2_187 - Urea ABC transporter, ATPase protein UrtE, at 239,918 to 240,607
_187
Pf6N2E2_188 - Formamidase (EC 3.5.1.49), at 240,740 to 241,969
_188
Position (kb) 239
240 Strain fitness (log2 ratio) -2
-1
0
1
2 at 238.366 kb on - strand, within Pf6N2E2_185 at 238.421 kb on + strand, within Pf6N2E2_185 at 238.421 kb on + strand, within Pf6N2E2_185 at 238.422 kb on - strand, within Pf6N2E2_185 at 238.422 kb on - strand, within Pf6N2E2_185 at 238.877 kb on + strand, within Pf6N2E2_185 at 238.877 kb on + strand, within Pf6N2E2_185 at 238.898 kb on + strand, within Pf6N2E2_185 at 238.899 kb on - strand, within Pf6N2E2_185 at 238.910 kb on + strand, within Pf6N2E2_185 at 238.911 kb on - strand, within Pf6N2E2_185 at 238.911 kb on - strand, within Pf6N2E2_185 at 238.944 kb on - strand, within Pf6N2E2_185 at 238.979 kb on + strand, within Pf6N2E2_185 at 238.980 kb on - strand, within Pf6N2E2_185 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.248 kb on + strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.249 kb on - strand, within Pf6N2E2_186 at 239.393 kb on - strand, within Pf6N2E2_186 at 239.393 kb on - strand, within Pf6N2E2_186 at 239.717 kb on + strand, within Pf6N2E2_186 at 239.808 kb on + strand, within Pf6N2E2_186 at 239.893 kb on + strand at 239.893 kb on + strand at 239.894 kb on - strand at 239.897 kb on + strand at 239.928 kb on + strand at 239.928 kb on + strand at 239.929 kb on - strand at 240.155 kb on + strand, within Pf6N2E2_187 at 240.155 kb on + strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.156 kb on - strand, within Pf6N2E2_187 at 240.185 kb on - strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.207 kb on + strand, within Pf6N2E2_187 at 240.257 kb on + strand, within Pf6N2E2_187 at 240.606 kb on - strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.626 kb on + strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.627 kb on - strand at 240.636 kb on + strand at 240.654 kb on + strand at 240.654 kb on + strand at 240.669 kb on - strand at 240.712 kb on + strand at 240.712 kb on + strand at 240.859 kb on + strand at 240.859 kb on + strand at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188 at 240.873 kb on - strand, within Pf6N2E2_188
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 238,366 - Pf6N2E2_185 0.34 -0.0 238,421 + Pf6N2E2_185 0.38 -1.0 238,421 + Pf6N2E2_185 0.38 +1.5 238,422 - Pf6N2E2_185 0.38 +0.4 238,422 - Pf6N2E2_185 0.38 +1.6 238,877 + Pf6N2E2_185 0.77 +0.1 238,877 + Pf6N2E2_185 0.77 +0.5 238,898 + Pf6N2E2_185 0.79 +1.0 238,899 - Pf6N2E2_185 0.79 -1.4 238,910 + Pf6N2E2_185 0.80 +1.0 238,911 - Pf6N2E2_185 0.80 +0.3 238,911 - Pf6N2E2_185 0.80 +0.6 238,944 - Pf6N2E2_185 0.82 +0.9 238,979 + Pf6N2E2_185 0.85 -0.1 238,980 - Pf6N2E2_185 0.85 +2.2 239,248 + Pf6N2E2_186 0.13 -0.9 239,248 + Pf6N2E2_186 0.13 +1.0 239,248 + Pf6N2E2_186 0.13 +0.8 239,249 - Pf6N2E2_186 0.13 +1.5 239,249 - Pf6N2E2_186 0.13 +0.4 239,249 - Pf6N2E2_186 0.13 +1.1 239,393 - Pf6N2E2_186 0.32 -0.1 239,393 - Pf6N2E2_186 0.32 +1.1 239,717 + Pf6N2E2_186 0.75 -1.0 239,808 + Pf6N2E2_186 0.87 +0.8 239,893 + +1.4 239,893 + -0.4 239,894 - +1.2 239,897 + +0.7 239,928 + +0.2 239,928 + -0.8 239,929 - +0.4 240,155 + Pf6N2E2_187 0.34 -0.9 240,155 + Pf6N2E2_187 0.34 +0.1 240,156 - Pf6N2E2_187 0.34 -1.0 240,156 - Pf6N2E2_187 0.34 +1.2 240,185 - Pf6N2E2_187 0.39 -1.6 240,207 + Pf6N2E2_187 0.42 +0.9 240,207 + Pf6N2E2_187 0.42 +0.7 240,207 + Pf6N2E2_187 0.42 +0.6 240,257 + Pf6N2E2_187 0.49 +0.3 240,606 - -0.3 240,626 + -0.4 240,626 + -0.0 240,626 + -2.0 240,627 - +1.0 240,627 - -0.7 240,627 - +0.6 240,627 - -1.0 240,636 + -0.5 240,654 + -1.8 240,654 + -0.4 240,669 - -0.2 240,712 + +1.0 240,712 + +0.8 240,859 + -0.4 240,859 + -0.0 240,873 - Pf6N2E2_188 0.11 -0.0 240,873 - Pf6N2E2_188 0.11 +1.8 240,873 - Pf6N2E2_188 0.11 +1.2
Or see this region's nucleotide sequence