Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_158 and Pf6N2E2_159 are separated by 5 nucleotides Pf6N2E2_159 and Pf6N2E2_160 are separated by 248 nucleotides Pf6N2E2_160 and Pf6N2E2_161 are separated by 133 nucleotides
Pf6N2E2_158 - Endonuclease I precursor (EC 3.1.21.1), at 212,509 to 213,198
_158
Pf6N2E2_159 - DNA damage resistance protein (from data) , at 213,204 to 213,452
_159
Pf6N2E2_160 - hypothetical protein, at 213,701 to 214,147
_160
Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237
_161
Position (kb)
213
214
215 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 212.882 kb on + strand, within Pf6N2E2_158 at 212.882 kb on + strand, within Pf6N2E2_158 at 212.883 kb on - strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.967 kb on + strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 212.968 kb on - strand, within Pf6N2E2_158 at 213.013 kb on + strand, within Pf6N2E2_158 at 213.014 kb on - strand, within Pf6N2E2_158 at 213.014 kb on - strand, within Pf6N2E2_158 at 213.017 kb on + strand, within Pf6N2E2_158 at 213.018 kb on - strand, within Pf6N2E2_158 at 213.018 kb on - strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.053 kb on + strand, within Pf6N2E2_158 at 213.058 kb on - strand, within Pf6N2E2_158 at 213.058 kb on - strand, within Pf6N2E2_158 at 213.068 kb on + strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.069 kb on - strand, within Pf6N2E2_158 at 213.092 kb on + strand, within Pf6N2E2_158 at 213.092 kb on + strand, within Pf6N2E2_158 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.420 kb on + strand, within Pf6N2E2_159 at 213.421 kb on - strand, within Pf6N2E2_159 at 213.428 kb on + strand at 213.580 kb on + strand at 213.592 kb on + strand at 213.592 kb on + strand at 213.593 kb on - strand at 213.995 kb on + strand, within Pf6N2E2_160 at 213.996 kb on - strand, within Pf6N2E2_160 at 214.069 kb on - strand, within Pf6N2E2_160 at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.197 kb on + strand at 214.198 kb on - strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.203 kb on - strand at 214.203 kb on - strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.258 kb on - strand at 214.258 kb on - strand at 214.264 kb on - strand at 214.264 kb on - strand at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.456 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 212,882 + Pf6N2E2_158 0.54 +0.4 212,882 + Pf6N2E2_158 0.54 +1.0 212,883 - Pf6N2E2_158 0.54 +0.4 212,967 + Pf6N2E2_158 0.66 +0.3 212,967 + Pf6N2E2_158 0.66 -1.6 212,967 + Pf6N2E2_158 0.66 -0.5 212,967 + Pf6N2E2_158 0.66 -0.5 212,967 + Pf6N2E2_158 0.66 +0.8 212,967 + Pf6N2E2_158 0.66 +0.3 212,968 - Pf6N2E2_158 0.67 -0.0 212,968 - Pf6N2E2_158 0.67 +2.0 212,968 - Pf6N2E2_158 0.67 +0.6 212,968 - Pf6N2E2_158 0.67 +1.3 212,968 - Pf6N2E2_158 0.67 -0.0 213,013 + Pf6N2E2_158 0.73 -2.0 213,014 - Pf6N2E2_158 0.73 +0.3 213,014 - Pf6N2E2_158 0.73 -2.7 213,017 + Pf6N2E2_158 0.74 -0.9 213,018 - Pf6N2E2_158 0.74 +1.4 213,018 - Pf6N2E2_158 0.74 -0.6 213,053 + Pf6N2E2_158 0.79 +1.7 213,053 + Pf6N2E2_158 0.79 -1.3 213,053 + Pf6N2E2_158 0.79 +0.1 213,058 - Pf6N2E2_158 0.80 -0.8 213,058 - Pf6N2E2_158 0.80 -0.0 213,068 + Pf6N2E2_158 0.81 +0.8 213,069 - Pf6N2E2_158 0.81 -0.1 213,069 - Pf6N2E2_158 0.81 -0.9 213,069 - Pf6N2E2_158 0.81 +1.3 213,069 - Pf6N2E2_158 0.81 -0.1 213,092 + Pf6N2E2_158 0.84 -0.4 213,092 + Pf6N2E2_158 0.84 -0.6 213,411 - Pf6N2E2_159 0.83 +0.7 213,411 - Pf6N2E2_159 0.83 +1.3 213,412 + Pf6N2E2_159 0.84 -0.8 213,412 + Pf6N2E2_159 0.84 +1.7 213,412 + Pf6N2E2_159 0.84 -1.3 213,413 - Pf6N2E2_159 0.84 -0.3 213,413 - Pf6N2E2_159 0.84 -1.8 213,420 + Pf6N2E2_159 0.87 +0.1 213,421 - Pf6N2E2_159 0.87 +0.1 213,428 + -1.2 213,580 + -0.9 213,592 + +0.8 213,592 + -2.5 213,593 - -1.8 213,995 + Pf6N2E2_160 0.66 -1.2 213,996 - Pf6N2E2_160 0.66 -1.9 214,069 - Pf6N2E2_160 0.82 +0.3 214,135 + -2.3 214,135 + +0.5 214,135 + -1.1 214,135 + +0.1 214,136 - -0.2 214,136 - -0.0 214,136 - -0.5 214,136 - +0.2 214,136 - -0.5 214,136 - -1.0 214,136 - +1.3 214,197 + +0.2 214,198 - +0.7 214,202 + -0.9 214,202 + -0.0 214,202 + +1.0 214,203 - +0.2 214,203 - +0.1 214,257 + -1.6 214,257 + +1.1 214,257 + +0.6 214,258 - -0.5 214,258 - +0.4 214,264 - +0.2 214,264 - +1.0 214,455 + Pf6N2E2_161 0.18 -1.0 214,455 + Pf6N2E2_161 0.18 +1.0 214,455 + Pf6N2E2_161 0.18 +1.0 214,456 - Pf6N2E2_161 0.18 -1.1 214,753 - Pf6N2E2_161 0.49 -0.0 214,753 - Pf6N2E2_161 0.49 -0.0
Or see this region's nucleotide sequence