Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotides BWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotides BWI76_RS18850 and BWI76_RS18855 are separated by 30 nucleotides
BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378
_RS18840
BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835
_RS18845
BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855
_RS18850
BWI76_RS18855: BWI76_RS18855 - cysteine desulfurase NifS, at 3,910,886 to 3,912,088
_RS18855
Position (kb)
3910
3911 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3909.394 kb on + strand at 3909.471 kb on - strand at 3909.538 kb on - strand, within BWI76_RS18845 at 3909.610 kb on + strand, within BWI76_RS18845 at 3909.735 kb on + strand, within BWI76_RS18845 at 3910.023 kb on - strand at 3910.273 kb on + strand, within BWI76_RS18850 at 3910.355 kb on - strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.489 kb on - strand, within BWI76_RS18850 at 3910.709 kb on + strand, within BWI76_RS18850 at 3910.712 kb on - strand, within BWI76_RS18850 at 3910.782 kb on + strand at 3911.309 kb on + strand, within BWI76_RS18855 at 3911.517 kb on + strand, within BWI76_RS18855 at 3911.567 kb on + strand, within BWI76_RS18855 at 3911.838 kb on + strand, within BWI76_RS18855
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x remove 3,909,394 + +0.1 3,909,471 - +2.8 3,909,538 - BWI76_RS18845 0.37 +0.0 3,909,610 + BWI76_RS18845 0.52 +0.1 3,909,735 + BWI76_RS18845 0.79 -0.3 3,910,023 - -0.0 3,910,273 + BWI76_RS18850 0.29 +0.3 3,910,355 - BWI76_RS18850 0.39 +0.1 3,910,481 + BWI76_RS18850 0.55 +0.6 3,910,481 + BWI76_RS18850 0.55 +0.1 3,910,481 + BWI76_RS18850 0.55 +1.0 3,910,489 - BWI76_RS18850 0.56 -0.3 3,910,709 + BWI76_RS18850 0.82 -0.0 3,910,712 - BWI76_RS18850 0.83 -0.1 3,910,782 + -0.0 3,911,309 + BWI76_RS18855 0.35 +0.8 3,911,517 + BWI76_RS18855 0.52 -0.8 3,911,567 + BWI76_RS18855 0.57 -0.5 3,911,838 + BWI76_RS18855 0.79 +2.1
Or see this region's nucleotide sequence