Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08230

Experiment: RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08225 and BWI76_RS08230 are separated by 99 nucleotidesBWI76_RS08230 and BWI76_RS08235 are separated by 92 nucleotides BWI76_RS08225: BWI76_RS08225 - glutamine--tRNA ligase, at 1,741,165 to 1,742,832 _RS08225 BWI76_RS08230: BWI76_RS08230 - hypothetical protein, at 1,742,932 to 1,743,176 _RS08230 BWI76_RS08235: BWI76_RS08235 - chitoporin, at 1,743,269 to 1,744,672 _RS08235 Position (kb) 1742 1743 1744Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1743.053 kb on + strand, within BWI76_RS08230at 1743.061 kb on - strand, within BWI76_RS08230at 1743.061 kb on - strand, within BWI76_RS08230at 1743.083 kb on - strand, within BWI76_RS08230at 1743.225 kb on + strandat 1743.250 kb on + strandat 1743.381 kb on + strandat 1743.540 kb on + strand, within BWI76_RS08235at 1743.554 kb on - strand, within BWI76_RS08235at 1743.597 kb on + strand, within BWI76_RS08235at 1743.609 kb on - strand, within BWI76_RS08235at 1743.667 kb on + strand, within BWI76_RS08235at 1743.667 kb on + strand, within BWI76_RS08235at 1743.675 kb on - strand, within BWI76_RS08235at 1743.675 kb on - strand, within BWI76_RS08235at 1743.716 kb on + strand, within BWI76_RS08235at 1743.782 kb on - strand, within BWI76_RS08235at 1743.886 kb on + strand, within BWI76_RS08235at 1744.000 kb on + strand, within BWI76_RS08235at 1744.000 kb on + strand, within BWI76_RS08235at 1744.008 kb on - strand, within BWI76_RS08235at 1744.008 kb on - strand, within BWI76_RS08235

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_glucose with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 1x
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1,743,053 + BWI76_RS08230 0.49 +0.4
1,743,061 - BWI76_RS08230 0.53 -2.1
1,743,061 - BWI76_RS08230 0.53 +0.3
1,743,083 - BWI76_RS08230 0.62 -1.1
1,743,225 + +0.2
1,743,250 + +1.1
1,743,381 + -2.0
1,743,540 + BWI76_RS08235 0.19 -1.6
1,743,554 - BWI76_RS08235 0.20 -1.1
1,743,597 + BWI76_RS08235 0.23 -0.3
1,743,609 - BWI76_RS08235 0.24 -0.9
1,743,667 + BWI76_RS08235 0.28 +0.1
1,743,667 + BWI76_RS08235 0.28 +2.8
1,743,675 - BWI76_RS08235 0.29 -0.5
1,743,675 - BWI76_RS08235 0.29 +0.5
1,743,716 + BWI76_RS08235 0.32 -1.1
1,743,782 - BWI76_RS08235 0.37 -0.0
1,743,886 + BWI76_RS08235 0.44 +0.5
1,744,000 + BWI76_RS08235 0.52 -1.3
1,744,000 + BWI76_RS08235 0.52 +0.0
1,744,008 - BWI76_RS08235 0.53 +0.1
1,744,008 - BWI76_RS08235 0.53 -0.3

Or see this region's nucleotide sequence