Experiment: Formate/Acetate-Sulfite (60/30-60 mM)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolQ-1 and tolR overlap by 17 nucleotides tolR and DVU2390 overlap by 4 nucleotides DVU2390 and tRNA-Asn are separated by 281 nucleotides tRNA-Asn and DVU2391 are separated by 151 nucleotides DVU2391 and DVU2392 are separated by 184 nucleotides
DVU2388: tolQ-1 - tolQ protein (TIGR), at 2,490,966 to 2,491,919
tolQ-1
DVU2389: tolR - biopolymer transport protein, ExbD/TolR family (TIGR), at 2,491,903 to 2,492,316
tolR
DVU2390: DVU2390 - TonB domain protein (TIGR), at 2,492,313 to 2,493,221
DVU2390
DVU_tRNA-Asn-2: tRNA-Asn - tRNA with anticodon GTT for Asn (VIMSS-AUTO), at 2,493,503 to 2,493,578
tRNA-Asn
DVU2391: DVU2391 - hypothetical protein (TIGR), at 2,493,730 to 2,493,873
DVU2391
DVU2392: DVU2392 - type III secretion chaperone, CesT family (TIGR), at 2,494,058 to 2,494,510
DVU2392
Position (kb)
2492
2493
2494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2491.373 kb on + strand, within tolQ-1 at 2491.373 kb on + strand, within tolQ-1 at 2491.383 kb on + strand, within tolQ-1 at 2491.383 kb on + strand, within tolQ-1 at 2491.421 kb on + strand, within tolQ-1 at 2491.429 kb on - strand, within tolQ-1 at 2491.429 kb on - strand, within tolQ-1 at 2491.532 kb on - strand, within tolQ-1 at 2491.578 kb on - strand, within tolQ-1 at 2491.905 kb on - strand at 2491.969 kb on + strand, within tolR at 2492.162 kb on + strand, within tolR at 2492.163 kb on + strand, within tolR at 2492.291 kb on - strand at 2492.312 kb on + strand at 2492.374 kb on + strand at 2492.374 kb on + strand at 2492.445 kb on - strand, within DVU2390 at 2492.450 kb on + strand, within DVU2390 at 2492.486 kb on + strand, within DVU2390 at 2492.494 kb on - strand, within DVU2390 at 2492.494 kb on - strand, within DVU2390 at 2492.552 kb on + strand, within DVU2390 at 2492.604 kb on - strand, within DVU2390 at 2492.636 kb on + strand, within DVU2390 at 2492.674 kb on - strand, within DVU2390 at 2492.693 kb on + strand, within DVU2390 at 2492.770 kb on + strand, within DVU2390 at 2492.819 kb on - strand, within DVU2390 at 2492.913 kb on + strand, within DVU2390 at 2492.934 kb on + strand, within DVU2390 at 2492.940 kb on + strand, within DVU2390 at 2492.950 kb on + strand, within DVU2390 at 2492.958 kb on - strand, within DVU2390 at 2493.003 kb on + strand, within DVU2390 at 2493.074 kb on - strand, within DVU2390 at 2493.363 kb on + strand at 2493.363 kb on + strand at 2493.376 kb on + strand at 2493.376 kb on + strand at 2493.384 kb on - strand at 2493.675 kb on + strand at 2493.832 kb on - strand, within DVU2391 at 2493.963 kb on + strand at 2493.983 kb on - strand at 2494.051 kb on - strand at 2494.170 kb on + strand, within DVU2392 at 2494.179 kb on - strand, within DVU2392 at 2494.199 kb on + strand, within DVU2392 at 2494.201 kb on - strand, within DVU2392 at 2494.214 kb on - strand, within DVU2392
Per-strain Table
Position Strand Gene LocusTag Fraction Formate/Acetate-Sulfite (60/30-60 mM) remove 2,491,373 + tolQ-1 DVU2388 0.43 -2.9 2,491,373 + tolQ-1 DVU2388 0.43 +0.0 2,491,383 + tolQ-1 DVU2388 0.44 -1.0 2,491,383 + tolQ-1 DVU2388 0.44 -2.1 2,491,421 + tolQ-1 DVU2388 0.48 -0.1 2,491,429 - tolQ-1 DVU2388 0.49 +0.9 2,491,429 - tolQ-1 DVU2388 0.49 -0.1 2,491,532 - tolQ-1 DVU2388 0.59 +0.4 2,491,578 - tolQ-1 DVU2388 0.64 +0.4 2,491,905 - -0.1 2,491,969 + tolR DVU2389 0.16 -0.6 2,492,162 + tolR DVU2389 0.63 -0.6 2,492,163 + tolR DVU2389 0.63 -0.1 2,492,291 - -1.1 2,492,312 + -0.1 2,492,374 + -0.2 2,492,374 + -1.0 2,492,445 - DVU2390 0.15 -1.4 2,492,450 + DVU2390 0.15 -0.3 2,492,486 + DVU2390 0.19 -0.0 2,492,494 - DVU2390 0.20 +0.6 2,492,494 - DVU2390 0.20 -0.2 2,492,552 + DVU2390 0.26 +2.0 2,492,604 - DVU2390 0.32 -0.6 2,492,636 + DVU2390 0.36 +1.4 2,492,674 - DVU2390 0.40 -0.1 2,492,693 + DVU2390 0.42 -0.3 2,492,770 + DVU2390 0.50 +0.7 2,492,819 - DVU2390 0.56 -0.3 2,492,913 + DVU2390 0.66 +0.0 2,492,934 + DVU2390 0.68 -0.4 2,492,940 + DVU2390 0.69 +0.2 2,492,950 + DVU2390 0.70 +1.0 2,492,958 - DVU2390 0.71 +0.2 2,493,003 + DVU2390 0.76 +0.9 2,493,074 - DVU2390 0.84 +1.7 2,493,363 + -0.8 2,493,363 + -0.4 2,493,376 + -0.1 2,493,376 + +0.0 2,493,384 - +0.2 2,493,675 + +0.2 2,493,832 - DVU2391 0.71 +0.2 2,493,963 + -0.5 2,493,983 - +0.1 2,494,051 - -0.6 2,494,170 + DVU2392 0.25 +0.4 2,494,179 - DVU2392 0.27 -1.2 2,494,199 + DVU2392 0.31 -0.2 2,494,201 - DVU2392 0.32 -0.1 2,494,214 - DVU2392 0.34 +0.0
Or see this region's nucleotide sequence