Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4474 and Psyr_4475 overlap by 4 nucleotides Psyr_4475 and Psyr_4476 overlap by 8 nucleotides Psyr_4476 and Psyr_4477 are separated by 153 nucleotides
Psyr_4474: Psyr_4474 - hypothetical protein, at 5,333,982 to 5,335,508
_4474
Psyr_4475: Psyr_4475 - NAD-dependent epimerase/dehydratase, at 5,335,505 to 5,336,434
_4475
Psyr_4476: Psyr_4476 - dTDP-4-dehydrorhamnose reductase, at 5,336,427 to 5,337,311
_4476
Psyr_4477: Psyr_4477 - MscS Mechanosensitive ion channel, at 5,337,465 to 5,339,783
_4477
Position (kb) 5336
5337
5338 Strain fitness (log2 ratio) -1
0
1
2
3 at 5335.499 kb on + strand at 5335.499 kb on + strand at 5335.499 kb on + strand at 5336.681 kb on - strand, within Psyr_4476 at 5336.681 kb on - strand, within Psyr_4476 at 5336.681 kb on - strand, within Psyr_4476 at 5336.878 kb on - strand, within Psyr_4476 at 5336.878 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.128 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.131 kb on - strand, within Psyr_4476 at 5337.466 kb on + strand at 5337.467 kb on - strand at 5337.522 kb on + strand at 5337.522 kb on + strand at 5337.523 kb on - strand at 5337.523 kb on - strand at 5337.632 kb on - strand at 5337.632 kb on - strand at 5337.709 kb on + strand, within Psyr_4477 at 5337.710 kb on - strand, within Psyr_4477 at 5337.728 kb on + strand, within Psyr_4477 at 5337.729 kb on - strand, within Psyr_4477 at 5337.729 kb on - strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.886 kb on + strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477 at 5337.887 kb on - strand, within Psyr_4477
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 5,335,499 + +0.3 5,335,499 + -0.2 5,335,499 + +0.3 5,336,681 - Psyr_4476 0.29 +1.4 5,336,681 - Psyr_4476 0.29 -1.0 5,336,681 - Psyr_4476 0.29 -1.6 5,336,878 - Psyr_4476 0.51 +1.1 5,336,878 - Psyr_4476 0.51 -0.8 5,337,128 - Psyr_4476 0.79 -1.0 5,337,128 - Psyr_4476 0.79 -1.2 5,337,128 - Psyr_4476 0.79 -0.5 5,337,131 - Psyr_4476 0.80 -1.1 5,337,131 - Psyr_4476 0.80 +0.2 5,337,131 - Psyr_4476 0.80 -1.3 5,337,131 - Psyr_4476 0.80 -0.5 5,337,131 - Psyr_4476 0.80 -0.5 5,337,131 - Psyr_4476 0.80 -1.3 5,337,131 - Psyr_4476 0.80 -0.7 5,337,131 - Psyr_4476 0.80 -1.1 5,337,466 + -1.1 5,337,467 - +1.1 5,337,522 + -0.7 5,337,522 + +3.4 5,337,523 - -0.4 5,337,523 - +2.6 5,337,632 - +0.8 5,337,632 - -0.6 5,337,709 + Psyr_4477 0.11 -0.6 5,337,710 - Psyr_4477 0.11 +1.1 5,337,728 + Psyr_4477 0.11 -0.3 5,337,729 - Psyr_4477 0.11 +0.9 5,337,729 - Psyr_4477 0.11 +1.0 5,337,886 + Psyr_4477 0.18 +0.0 5,337,886 + Psyr_4477 0.18 +0.6 5,337,886 + Psyr_4477 0.18 -0.9 5,337,887 - Psyr_4477 0.18 +0.3 5,337,887 - Psyr_4477 0.18 -1.4 5,337,887 - Psyr_4477 0.18 +1.3
Or see this region's nucleotide sequence