Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_4391

Experiment: library passed through aphids in food, 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_4390 and Psyr_4391 are separated by 20 nucleotidesPsyr_4391 and Psyr_4392 overlap by 4 nucleotides Psyr_4390: Psyr_4390 - Flp pilus assembly CpaB, at 5,251,748 to 5,252,707 _4390 Psyr_4391: Psyr_4391 - type II and III secretion system protein, at 5,252,728 to 5,253,978 _4391 Psyr_4392: Psyr_4392 - pilus assembly protein CpaE, at 5,253,975 to 5,255,165 _4392 Position (kb) 5252 5253 5254Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5251.811 kb on + strandat 5251.811 kb on + strandat 5251.812 kb on - strandat 5251.973 kb on + strand, within Psyr_4390at 5251.973 kb on + strand, within Psyr_4390at 5251.973 kb on + strand, within Psyr_4390at 5251.974 kb on - strand, within Psyr_4390at 5251.974 kb on - strand, within Psyr_4390at 5252.222 kb on + strand, within Psyr_4390at 5252.223 kb on - strand, within Psyr_4390at 5252.232 kb on + strand, within Psyr_4390at 5252.232 kb on + strand, within Psyr_4390at 5252.232 kb on + strand, within Psyr_4390at 5252.232 kb on + strand, within Psyr_4390at 5252.232 kb on + strand, within Psyr_4390at 5252.233 kb on - strand, within Psyr_4390at 5252.233 kb on - strand, within Psyr_4390at 5252.233 kb on - strand, within Psyr_4390at 5252.233 kb on - strand, within Psyr_4390at 5252.233 kb on - strand, within Psyr_4390at 5252.241 kb on - strand, within Psyr_4390at 5252.241 kb on - strand, within Psyr_4390at 5252.241 kb on - strand, within Psyr_4390at 5252.241 kb on - strand, within Psyr_4390at 5252.241 kb on - strand, within Psyr_4390at 5252.522 kb on + strand, within Psyr_4390at 5252.650 kb on + strandat 5252.651 kb on - strandat 5252.651 kb on - strandat 5252.651 kb on - strandat 5252.651 kb on - strandat 5252.751 kb on + strandat 5252.751 kb on + strandat 5252.751 kb on + strandat 5252.752 kb on - strandat 5252.752 kb on - strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.753 kb on + strandat 5252.754 kb on - strandat 5252.754 kb on - strandat 5252.754 kb on - strandat 5253.183 kb on + strand, within Psyr_4391at 5253.386 kb on - strand, within Psyr_4391at 5253.386 kb on - strand, within Psyr_4391at 5253.490 kb on - strand, within Psyr_4391at 5253.502 kb on + strand, within Psyr_4391at 5253.502 kb on + strand, within Psyr_4391at 5253.502 kb on + strand, within Psyr_4391at 5253.502 kb on + strand, within Psyr_4391at 5253.503 kb on - strand, within Psyr_4391at 5253.904 kb on + strandat 5253.904 kb on + strandat 5253.904 kb on + strandat 5253.904 kb on + strandat 5253.904 kb on + strandat 5253.904 kb on + strandat 5253.905 kb on - strandat 5254.022 kb on + strandat 5254.479 kb on - strand, within Psyr_4392at 5254.644 kb on + strand, within Psyr_4392at 5254.645 kb on - strand, within Psyr_4392at 5254.645 kb on - strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.692 kb on + strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.693 kb on - strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.694 kb on + strand, within Psyr_4392at 5254.695 kb on - strand, within Psyr_4392at 5254.695 kb on - strand, within Psyr_4392at 5254.695 kb on - strand, within Psyr_4392at 5254.695 kb on - strand, within Psyr_4392at 5254.748 kb on - strand, within Psyr_4392at 5254.748 kb on - strand, within Psyr_4392at 5254.748 kb on - strand, within Psyr_4392at 5254.809 kb on + strand, within Psyr_4392at 5254.810 kb on - strand, within Psyr_4392

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Per-strain Table

Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours
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5,251,811 + -0.0
5,251,811 + +0.8
5,251,812 - +0.3
5,251,973 + Psyr_4390 0.23 -1.4
5,251,973 + Psyr_4390 0.23 +1.3
5,251,973 + Psyr_4390 0.23 -0.4
5,251,974 - Psyr_4390 0.24 -2.2
5,251,974 - Psyr_4390 0.24 -2.7
5,252,222 + Psyr_4390 0.49 -0.3
5,252,223 - Psyr_4390 0.49 +0.9
5,252,232 + Psyr_4390 0.50 +1.4
5,252,232 + Psyr_4390 0.50 +0.8
5,252,232 + Psyr_4390 0.50 -0.1
5,252,232 + Psyr_4390 0.50 +0.9
5,252,232 + Psyr_4390 0.50 +1.4
5,252,233 - Psyr_4390 0.51 +1.1
5,252,233 - Psyr_4390 0.51 +0.6
5,252,233 - Psyr_4390 0.51 -0.4
5,252,233 - Psyr_4390 0.51 +2.2
5,252,233 - Psyr_4390 0.51 +1.4
5,252,241 - Psyr_4390 0.51 +0.8
5,252,241 - Psyr_4390 0.51 +0.9
5,252,241 - Psyr_4390 0.51 +0.6
5,252,241 - Psyr_4390 0.51 -0.4
5,252,241 - Psyr_4390 0.51 +0.3
5,252,522 + Psyr_4390 0.81 -0.4
5,252,650 + +2.1
5,252,651 - +0.5
5,252,651 - +0.8
5,252,651 - -0.1
5,252,651 - +1.9
5,252,751 + +0.6
5,252,751 + +1.8
5,252,751 + +0.5
5,252,752 - +0.7
5,252,752 - +1.6
5,252,753 + +0.4
5,252,753 + +0.7
5,252,753 + -1.5
5,252,753 + +0.9
5,252,753 + +1.4
5,252,753 + +0.2
5,252,753 + +0.2
5,252,754 - -0.3
5,252,754 - -0.4
5,252,754 - +0.8
5,253,183 + Psyr_4391 0.36 -0.3
5,253,386 - Psyr_4391 0.53 +0.8
5,253,386 - Psyr_4391 0.53 +1.6
5,253,490 - Psyr_4391 0.61 +0.6
5,253,502 + Psyr_4391 0.62 +0.6
5,253,502 + Psyr_4391 0.62 +1.5
5,253,502 + Psyr_4391 0.62 -0.3
5,253,502 + Psyr_4391 0.62 +1.6
5,253,503 - Psyr_4391 0.62 -0.1
5,253,904 + -0.7
5,253,904 + +0.4
5,253,904 + -0.4
5,253,904 + -0.1
5,253,904 + -0.1
5,253,904 + +1.9
5,253,905 - +1.6
5,254,022 + -1.4
5,254,479 - Psyr_4392 0.42 +0.0
5,254,644 + Psyr_4392 0.56 +1.1
5,254,645 - Psyr_4392 0.56 +0.1
5,254,645 - Psyr_4392 0.56 -1.9
5,254,692 + Psyr_4392 0.60 -1.5
5,254,692 + Psyr_4392 0.60 +0.5
5,254,692 + Psyr_4392 0.60 +1.0
5,254,692 + Psyr_4392 0.60 +0.9
5,254,692 + Psyr_4392 0.60 -1.5
5,254,692 + Psyr_4392 0.60 +0.4
5,254,692 + Psyr_4392 0.60 -0.8
5,254,692 + Psyr_4392 0.60 +0.6
5,254,692 + Psyr_4392 0.60 -0.2
5,254,692 + Psyr_4392 0.60 -1.1
5,254,692 + Psyr_4392 0.60 +1.1
5,254,692 + Psyr_4392 0.60 +0.1
5,254,692 + Psyr_4392 0.60 -0.6
5,254,692 + Psyr_4392 0.60 -0.4
5,254,692 + Psyr_4392 0.60 -0.3
5,254,692 + Psyr_4392 0.60 +0.2
5,254,692 + Psyr_4392 0.60 -2.4
5,254,692 + Psyr_4392 0.60 -0.3
5,254,693 - Psyr_4392 0.60 +0.7
5,254,693 - Psyr_4392 0.60 -1.1
5,254,693 - Psyr_4392 0.60 -0.2
5,254,693 - Psyr_4392 0.60 -0.4
5,254,693 - Psyr_4392 0.60 -1.4
5,254,693 - Psyr_4392 0.60 +1.0
5,254,693 - Psyr_4392 0.60 -0.0
5,254,693 - Psyr_4392 0.60 +1.2
5,254,693 - Psyr_4392 0.60 -0.3
5,254,693 - Psyr_4392 0.60 -1.8
5,254,693 - Psyr_4392 0.60 -0.4
5,254,693 - Psyr_4392 0.60 +0.2
5,254,693 - Psyr_4392 0.60 -1.0
5,254,694 + Psyr_4392 0.60 +0.8
5,254,694 + Psyr_4392 0.60 -0.0
5,254,694 + Psyr_4392 0.60 +1.7
5,254,694 + Psyr_4392 0.60 +0.1
5,254,694 + Psyr_4392 0.60 +0.1
5,254,694 + Psyr_4392 0.60 +1.6
5,254,694 + Psyr_4392 0.60 -2.3
5,254,695 - Psyr_4392 0.60 +1.2
5,254,695 - Psyr_4392 0.60 -1.8
5,254,695 - Psyr_4392 0.60 +0.9
5,254,695 - Psyr_4392 0.60 -0.9
5,254,748 - Psyr_4392 0.65 +0.2
5,254,748 - Psyr_4392 0.65 +0.4
5,254,748 - Psyr_4392 0.65 +0.6
5,254,809 + Psyr_4392 0.70 +2.1
5,254,810 - Psyr_4392 0.70 +4.0

Or see this region's nucleotide sequence