Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4147 and Psyr_4148 are separated by 75 nucleotides Psyr_4148 and Psyr_4149 are separated by 10 nucleotides Psyr_4149 and Psyr_4150 are separated by 2 nucleotides Psyr_4150 and Psyr_4151 are separated by 15 nucleotides Psyr_4151 and Psyr_4152 are separated by 186 nucleotides
Psyr_4147: Psyr_4147 - RNA polymerase, sigma 54 subunit, RpoN/SigL, at 4,934,535 to 4,936,070
_4147
Psyr_4148: Psyr_4148 - sigma 54 modulation protein / SSU ribosomal protein S30P, at 4,936,146 to 4,936,454
_4148
Psyr_4149: Psyr_4149 - PTS IIA-like nitrogen-regulatory protein PtsN, at 4,936,465 to 4,936,929
_4149
Psyr_4150: Psyr_4150 - Uncharacterized P-loop ATPase protein UPF0042, at 4,936,932 to 4,937,789
_4150
Psyr_4151: Psyr_4151 - Phosphocarrier HPr protein, at 4,937,805 to 4,938,077
_4151
Psyr_4152: Psyr_4152 - Superoxide dismutase, at 4,938,264 to 4,938,875
_4152
Position (kb) 4936
4937
4938 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4936.120 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.145 kb on + strand at 4936.200 kb on + strand, within Psyr_4148 at 4936.247 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.356 kb on + strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.357 kb on - strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.387 kb on + strand, within Psyr_4148 at 4936.409 kb on + strand, within Psyr_4148 at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.463 kb on + strand at 4936.976 kb on + strand at 4937.010 kb on + strand at 4937.107 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.164 kb on + strand, within Psyr_4150 at 4937.165 kb on - strand, within Psyr_4150 at 4937.177 kb on + strand, within Psyr_4150 at 4937.197 kb on - strand, within Psyr_4150 at 4937.294 kb on - strand, within Psyr_4150 at 4937.294 kb on - strand, within Psyr_4150 at 4937.371 kb on - strand, within Psyr_4150 at 4937.401 kb on + strand, within Psyr_4150 at 4937.515 kb on - strand, within Psyr_4150 at 4937.568 kb on + strand, within Psyr_4150 at 4937.568 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.664 kb on + strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.665 kb on - strand, within Psyr_4150 at 4937.669 kb on + strand, within Psyr_4150 at 4937.687 kb on + strand, within Psyr_4150 at 4937.940 kb on - strand, within Psyr_4151 at 4938.075 kb on + strand at 4938.075 kb on + strand at 4938.075 kb on + strand at 4938.075 kb on + strand at 4938.075 kb on + strand at 4938.075 kb on + strand at 4938.076 kb on - strand at 4938.076 kb on - strand at 4938.236 kb on + strand at 4938.237 kb on - strand at 4938.320 kb on - strand at 4938.320 kb on - strand at 4938.320 kb on - strand at 4938.334 kb on + strand, within Psyr_4152 at 4938.334 kb on + strand, within Psyr_4152 at 4938.334 kb on + strand, within Psyr_4152 at 4938.334 kb on + strand, within Psyr_4152 at 4938.334 kb on + strand, within Psyr_4152 at 4938.334 kb on + strand, within Psyr_4152 at 4938.364 kb on + strand, within Psyr_4152 at 4938.364 kb on + strand, within Psyr_4152 at 4938.364 kb on + strand, within Psyr_4152 at 4938.367 kb on + strand, within Psyr_4152 at 4938.367 kb on + strand, within Psyr_4152 at 4938.367 kb on + strand, within Psyr_4152 at 4938.368 kb on - strand, within Psyr_4152 at 4938.368 kb on - strand, within Psyr_4152 at 4938.368 kb on - strand, within Psyr_4152 at 4938.368 kb on - strand, within Psyr_4152 at 4938.368 kb on - strand, within Psyr_4152 at 4938.435 kb on - strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.772 kb on + strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152 at 4938.773 kb on - strand, within Psyr_4152
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 4,936,120 + +0.1 4,936,145 + +0.2 4,936,145 + -0.0 4,936,145 + +1.9 4,936,145 + -0.9 4,936,200 + Psyr_4148 0.17 -0.7 4,936,247 + Psyr_4148 0.33 +1.0 4,936,356 + Psyr_4148 0.68 -1.0 4,936,356 + Psyr_4148 0.68 -0.4 4,936,356 + Psyr_4148 0.68 +1.9 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 -2.3 4,936,356 + Psyr_4148 0.68 -0.6 4,936,356 + Psyr_4148 0.68 +3.8 4,936,356 + Psyr_4148 0.68 +0.7 4,936,356 + Psyr_4148 0.68 -0.7 4,936,356 + Psyr_4148 0.68 +0.7 4,936,356 + Psyr_4148 0.68 -0.1 4,936,356 + Psyr_4148 0.68 +1.2 4,936,356 + Psyr_4148 0.68 +0.9 4,936,356 + Psyr_4148 0.68 -1.6 4,936,356 + Psyr_4148 0.68 +0.5 4,936,356 + Psyr_4148 0.68 -0.1 4,936,356 + Psyr_4148 0.68 -0.5 4,936,356 + Psyr_4148 0.68 -0.4 4,936,356 + Psyr_4148 0.68 +0.4 4,936,356 + Psyr_4148 0.68 +0.8 4,936,356 + Psyr_4148 0.68 -0.7 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +0.6 4,936,356 + Psyr_4148 0.68 -0.2 4,936,356 + Psyr_4148 0.68 -1.5 4,936,356 + Psyr_4148 0.68 +0.5 4,936,356 + Psyr_4148 0.68 -0.8 4,936,356 + Psyr_4148 0.68 +2.3 4,936,356 + Psyr_4148 0.68 -1.0 4,936,356 + Psyr_4148 0.68 +0.3 4,936,356 + Psyr_4148 0.68 +1.5 4,936,356 + Psyr_4148 0.68 -0.2 4,936,356 + Psyr_4148 0.68 -0.6 4,936,356 + Psyr_4148 0.68 +1.5 4,936,356 + Psyr_4148 0.68 +0.8 4,936,356 + Psyr_4148 0.68 -1.5 4,936,357 - Psyr_4148 0.68 +0.5 4,936,357 - Psyr_4148 0.68 +0.2 4,936,357 - Psyr_4148 0.68 -1.4 4,936,357 - Psyr_4148 0.68 -0.3 4,936,357 - Psyr_4148 0.68 -1.0 4,936,387 + Psyr_4148 0.78 -0.6 4,936,387 + Psyr_4148 0.78 +0.2 4,936,387 + Psyr_4148 0.78 +0.3 4,936,409 + Psyr_4148 0.85 -1.6 4,936,463 + -0.6 4,936,463 + -1.2 4,936,463 + -1.0 4,936,463 + -0.1 4,936,976 + +0.4 4,937,010 + -1.0 4,937,107 + Psyr_4150 0.20 +1.7 4,937,164 + Psyr_4150 0.27 -1.2 4,937,164 + Psyr_4150 0.27 -0.6 4,937,164 + Psyr_4150 0.27 -1.3 4,937,165 - Psyr_4150 0.27 -1.4 4,937,177 + Psyr_4150 0.29 -0.2 4,937,197 - Psyr_4150 0.31 +1.4 4,937,294 - Psyr_4150 0.42 -1.2 4,937,294 - Psyr_4150 0.42 -1.0 4,937,371 - Psyr_4150 0.51 -2.9 4,937,401 + Psyr_4150 0.55 -0.7 4,937,515 - Psyr_4150 0.68 -0.3 4,937,568 + Psyr_4150 0.74 -0.3 4,937,568 + Psyr_4150 0.74 -0.6 4,937,664 + Psyr_4150 0.85 -1.3 4,937,664 + Psyr_4150 0.85 -1.0 4,937,664 + Psyr_4150 0.85 +0.3 4,937,664 + Psyr_4150 0.85 -0.4 4,937,664 + Psyr_4150 0.85 +0.4 4,937,664 + Psyr_4150 0.85 -1.6 4,937,664 + Psyr_4150 0.85 -0.8 4,937,664 + Psyr_4150 0.85 -1.1 4,937,664 + Psyr_4150 0.85 -1.2 4,937,665 - Psyr_4150 0.85 -1.7 4,937,665 - Psyr_4150 0.85 -1.7 4,937,665 - Psyr_4150 0.85 -0.7 4,937,665 - Psyr_4150 0.85 -0.5 4,937,665 - Psyr_4150 0.85 -2.8 4,937,665 - Psyr_4150 0.85 -0.5 4,937,665 - Psyr_4150 0.85 -1.8 4,937,669 + Psyr_4150 0.86 -0.6 4,937,687 + Psyr_4150 0.88 -2.8 4,937,940 - Psyr_4151 0.49 -0.8 4,938,075 + +0.5 4,938,075 + -0.0 4,938,075 + -1.0 4,938,075 + +0.5 4,938,075 + +0.1 4,938,075 + -0.3 4,938,076 - +0.5 4,938,076 - +1.5 4,938,236 + -1.5 4,938,237 - +0.0 4,938,320 - -1.5 4,938,320 - +0.5 4,938,320 - -0.8 4,938,334 + Psyr_4152 0.11 -2.0 4,938,334 + Psyr_4152 0.11 -0.2 4,938,334 + Psyr_4152 0.11 -0.0 4,938,334 + Psyr_4152 0.11 -2.1 4,938,334 + Psyr_4152 0.11 -1.2 4,938,334 + Psyr_4152 0.11 +1.2 4,938,364 + Psyr_4152 0.16 -0.3 4,938,364 + Psyr_4152 0.16 -0.5 4,938,364 + Psyr_4152 0.16 +1.6 4,938,367 + Psyr_4152 0.17 +0.5 4,938,367 + Psyr_4152 0.17 -1.3 4,938,367 + Psyr_4152 0.17 -0.5 4,938,368 - Psyr_4152 0.17 -0.0 4,938,368 - Psyr_4152 0.17 -0.1 4,938,368 - Psyr_4152 0.17 -0.1 4,938,368 - Psyr_4152 0.17 -1.5 4,938,368 - Psyr_4152 0.17 -0.6 4,938,435 - Psyr_4152 0.28 +1.4 4,938,772 + Psyr_4152 0.83 +0.4 4,938,772 + Psyr_4152 0.83 -1.0 4,938,772 + Psyr_4152 0.83 -1.5 4,938,772 + Psyr_4152 0.83 +0.6 4,938,772 + Psyr_4152 0.83 +0.8 4,938,772 + Psyr_4152 0.83 -0.7 4,938,772 + Psyr_4152 0.83 -0.8 4,938,772 + Psyr_4152 0.83 -0.7 4,938,772 + Psyr_4152 0.83 -1.8 4,938,773 - Psyr_4152 0.83 -0.8 4,938,773 - Psyr_4152 0.83 -0.8 4,938,773 - Psyr_4152 0.83 +0.4 4,938,773 - Psyr_4152 0.83 -1.3 4,938,773 - Psyr_4152 0.83 -1.3 4,938,773 - Psyr_4152 0.83 +1.0 4,938,773 - Psyr_4152 0.83 +0.3
Or see this region's nucleotide sequence