Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3244 and Psyr_3245 are separated by 86 nucleotides Psyr_3245 and Psyr_3246 are separated by 20 nucleotides
Psyr_3244: Psyr_3244 - Phospholipase D/Transphosphatidylase, at 3,884,704 to 3,886,047
_3244
Psyr_3245: Psyr_3245 - ATP-dependent DNA ligase LigD phosphoesterase module / ATP-dependent DNA ligase LigD polymerase module, at 3,886,134 to 3,888,734
_3245
Psyr_3246: Psyr_3246 - Ku, at 3,888,755 to 3,889,660
_3246
Position (kb)
3886
3887
3888
3889 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.136 kb on + strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.137 kb on - strand, within Psyr_3244 at 3885.164 kb on - strand, within Psyr_3244 at 3885.180 kb on + strand, within Psyr_3244 at 3885.184 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.260 kb on + strand, within Psyr_3244 at 3885.261 kb on - strand, within Psyr_3244 at 3885.261 kb on - strand, within Psyr_3244 at 3885.508 kb on + strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.509 kb on - strand, within Psyr_3244 at 3885.655 kb on + strand, within Psyr_3244 at 3885.656 kb on - strand, within Psyr_3244 at 3885.661 kb on + strand, within Psyr_3244 at 3885.661 kb on + strand, within Psyr_3244 at 3885.790 kb on + strand, within Psyr_3244 at 3885.790 kb on + strand, within Psyr_3244 at 3885.943 kb on + strand at 3885.944 kb on - strand at 3885.944 kb on - strand at 3885.944 kb on - strand at 3885.971 kb on - strand at 3886.076 kb on + strand at 3886.076 kb on + strand at 3886.077 kb on - strand at 3886.100 kb on + strand at 3886.100 kb on + strand at 3886.101 kb on - strand at 3886.101 kb on - strand at 3886.101 kb on - strand at 3886.703 kb on + strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.704 kb on - strand, within Psyr_3245 at 3886.939 kb on + strand, within Psyr_3245 at 3887.020 kb on - strand, within Psyr_3245 at 3887.020 kb on - strand, within Psyr_3245 at 3887.313 kb on - strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.365 kb on + strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.366 kb on - strand, within Psyr_3245 at 3887.442 kb on - strand, within Psyr_3245 at 3887.515 kb on + strand, within Psyr_3245 at 3887.516 kb on - strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.725 kb on + strand, within Psyr_3245 at 3887.728 kb on + strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.729 kb on - strand, within Psyr_3245 at 3887.971 kb on + strand, within Psyr_3245 at 3887.972 kb on - strand, within Psyr_3245 at 3888.365 kb on - strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.433 kb on + strand, within Psyr_3245 at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.712 kb on + strand at 3888.713 kb on - strand at 3888.778 kb on + strand at 3888.778 kb on + strand at 3888.779 kb on - strand at 3888.779 kb on - strand at 3889.261 kb on + strand, within Psyr_3246 at 3889.293 kb on + strand, within Psyr_3246 at 3889.383 kb on - strand, within Psyr_3246 at 3889.446 kb on + strand, within Psyr_3246 at 3889.447 kb on - strand, within Psyr_3246 at 3889.527 kb on + strand, within Psyr_3246 at 3889.610 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.612 kb on + strand at 3889.613 kb on - strand at 3889.613 kb on - strand at 3889.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,885,136 + Psyr_3244 0.32 +0.4 3,885,136 + Psyr_3244 0.32 -1.7 3,885,136 + Psyr_3244 0.32 -0.3 3,885,136 + Psyr_3244 0.32 -0.4 3,885,137 - Psyr_3244 0.32 -0.6 3,885,137 - Psyr_3244 0.32 -0.6 3,885,137 - Psyr_3244 0.32 -1.4 3,885,137 - Psyr_3244 0.32 +0.0 3,885,137 - Psyr_3244 0.32 -0.6 3,885,164 - Psyr_3244 0.34 -0.2 3,885,180 + Psyr_3244 0.35 +1.5 3,885,184 + Psyr_3244 0.36 -0.5 3,885,260 + Psyr_3244 0.41 +0.5 3,885,260 + Psyr_3244 0.41 -0.2 3,885,261 - Psyr_3244 0.41 -0.2 3,885,261 - Psyr_3244 0.41 +1.4 3,885,508 + Psyr_3244 0.60 +0.5 3,885,509 - Psyr_3244 0.60 -0.7 3,885,509 - Psyr_3244 0.60 +0.5 3,885,509 - Psyr_3244 0.60 -1.7 3,885,655 + Psyr_3244 0.71 +2.0 3,885,656 - Psyr_3244 0.71 -0.3 3,885,661 + Psyr_3244 0.71 +0.2 3,885,661 + Psyr_3244 0.71 +0.1 3,885,790 + Psyr_3244 0.81 +0.3 3,885,790 + Psyr_3244 0.81 -0.4 3,885,943 + +0.8 3,885,944 - -1.8 3,885,944 - -0.5 3,885,944 - +0.3 3,885,971 - -1.5 3,886,076 + +0.9 3,886,076 + +0.5 3,886,077 - +1.7 3,886,100 + -0.4 3,886,100 + +1.1 3,886,101 - -0.1 3,886,101 - +0.4 3,886,101 - +3.2 3,886,703 + Psyr_3245 0.22 +1.7 3,886,704 - Psyr_3245 0.22 +0.1 3,886,704 - Psyr_3245 0.22 +0.2 3,886,704 - Psyr_3245 0.22 +0.6 3,886,939 + Psyr_3245 0.31 -0.6 3,887,020 - Psyr_3245 0.34 -0.5 3,887,020 - Psyr_3245 0.34 -1.9 3,887,313 - Psyr_3245 0.45 +0.9 3,887,365 + Psyr_3245 0.47 +0.3 3,887,365 + Psyr_3245 0.47 -1.1 3,887,365 + Psyr_3245 0.47 +0.5 3,887,366 - Psyr_3245 0.47 +0.0 3,887,366 - Psyr_3245 0.47 -0.1 3,887,366 - Psyr_3245 0.47 -0.4 3,887,366 - Psyr_3245 0.47 -0.6 3,887,442 - Psyr_3245 0.50 -0.5 3,887,515 + Psyr_3245 0.53 +7.1 3,887,516 - Psyr_3245 0.53 -0.8 3,887,725 + Psyr_3245 0.61 -0.4 3,887,725 + Psyr_3245 0.61 +0.6 3,887,725 + Psyr_3245 0.61 +0.8 3,887,725 + Psyr_3245 0.61 -0.4 3,887,728 + Psyr_3245 0.61 +1.9 3,887,729 - Psyr_3245 0.61 +0.5 3,887,729 - Psyr_3245 0.61 -1.3 3,887,729 - Psyr_3245 0.61 +0.4 3,887,971 + Psyr_3245 0.71 +1.5 3,887,972 - Psyr_3245 0.71 -1.4 3,888,365 - Psyr_3245 0.86 +1.9 3,888,433 + Psyr_3245 0.88 -1.2 3,888,433 + Psyr_3245 0.88 +1.2 3,888,712 + +1.5 3,888,712 + +0.4 3,888,712 + -1.1 3,888,712 + +7.1 3,888,713 - +1.4 3,888,778 + +0.9 3,888,778 + +0.1 3,888,779 - -0.5 3,888,779 - -0.6 3,889,261 + Psyr_3246 0.56 -1.6 3,889,293 + Psyr_3246 0.59 +1.1 3,889,383 - Psyr_3246 0.69 -1.6 3,889,446 + Psyr_3246 0.76 +0.9 3,889,447 - Psyr_3246 0.76 +2.2 3,889,527 + Psyr_3246 0.85 -2.3 3,889,610 + -0.5 3,889,612 + +1.6 3,889,612 + -0.4 3,889,612 + -0.4 3,889,612 + -0.4 3,889,612 + -1.4 3,889,612 + +0.1 3,889,612 + +0.4 3,889,613 - +1.2 3,889,613 - -2.0 3,889,613 - -1.0
Or see this region's nucleotide sequence