Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2820 and Psyr_2821 overlap by 4 nucleotides Psyr_2821 and Psyr_2822 overlap by 4 nucleotides Psyr_2822 and Psyr_2823 are separated by 81 nucleotides
Psyr_2820: Psyr_2820 - RecT protein, at 3,394,228 to 3,395,115
_2820
Psyr_2821: Psyr_2821 - conserved hypothetical protein, at 3,395,112 to 3,396,773
_2821
Psyr_2822: Psyr_2822 - hypothetical protein, at 3,396,770 to 3,397,132
_2822
Psyr_2823: Psyr_2823 - hypothetical protein, at 3,397,214 to 3,397,597
_2823
Position (kb)
3395
3396
3397 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3394.177 kb on - strand at 3394.227 kb on + strand at 3394.227 kb on + strand at 3394.228 kb on - strand at 3394.316 kb on + strand at 3394.534 kb on - strand, within Psyr_2820 at 3394.831 kb on + strand, within Psyr_2820 at 3394.832 kb on - strand, within Psyr_2820 at 3394.945 kb on + strand, within Psyr_2820 at 3395.047 kb on + strand at 3395.048 kb on - strand at 3395.048 kb on - strand at 3395.123 kb on + strand at 3395.169 kb on + strand at 3395.170 kb on - strand at 3395.289 kb on + strand, within Psyr_2821 at 3395.289 kb on + strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.290 kb on - strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.502 kb on + strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.503 kb on - strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.505 kb on + strand, within Psyr_2821 at 3395.602 kb on - strand, within Psyr_2821 at 3395.674 kb on - strand, within Psyr_2821 at 3395.747 kb on - strand, within Psyr_2821 at 3396.599 kb on + strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.600 kb on - strand, within Psyr_2821 at 3396.868 kb on - strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.938 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.962 kb on + strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3396.963 kb on - strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.094 kb on + strand, within Psyr_2822 at 3397.106 kb on + strand at 3397.211 kb on + strand at 3397.558 kb on + strand, within Psyr_2823 at 3397.559 kb on - strand at 3397.616 kb on + strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.617 kb on - strand at 3397.621 kb on - strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.664 kb on + strand at 3397.665 kb on - strand at 3397.721 kb on + strand at 3397.757 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,394,177 - -0.8 3,394,227 + -0.6 3,394,227 + -0.2 3,394,228 - +1.0 3,394,316 + +0.1 3,394,534 - Psyr_2820 0.34 +0.2 3,394,831 + Psyr_2820 0.68 -0.4 3,394,832 - Psyr_2820 0.68 -1.9 3,394,945 + Psyr_2820 0.81 +1.6 3,395,047 + +2.7 3,395,048 - -2.7 3,395,048 - +1.6 3,395,123 + -0.2 3,395,169 + +2.0 3,395,170 - -0.8 3,395,289 + Psyr_2821 0.11 -0.3 3,395,289 + Psyr_2821 0.11 +1.4 3,395,290 - Psyr_2821 0.11 -2.6 3,395,290 - Psyr_2821 0.11 -0.7 3,395,290 - Psyr_2821 0.11 -2.1 3,395,290 - Psyr_2821 0.11 +0.4 3,395,290 - Psyr_2821 0.11 -1.3 3,395,290 - Psyr_2821 0.11 +0.5 3,395,502 + Psyr_2821 0.23 -0.1 3,395,502 + Psyr_2821 0.23 -1.7 3,395,503 - Psyr_2821 0.24 -0.6 3,395,503 - Psyr_2821 0.24 -0.8 3,395,503 - Psyr_2821 0.24 +0.0 3,395,505 + Psyr_2821 0.24 +0.3 3,395,505 + Psyr_2821 0.24 +0.9 3,395,602 - Psyr_2821 0.29 -1.4 3,395,674 - Psyr_2821 0.34 -1.0 3,395,747 - Psyr_2821 0.38 +1.1 3,396,599 + Psyr_2821 0.89 -0.7 3,396,600 - Psyr_2821 0.90 -0.9 3,396,600 - Psyr_2821 0.90 -1.4 3,396,600 - Psyr_2821 0.90 +0.7 3,396,600 - Psyr_2821 0.90 +1.1 3,396,600 - Psyr_2821 0.90 +0.1 3,396,868 - Psyr_2822 0.27 -1.2 3,396,938 + Psyr_2822 0.46 -0.3 3,396,938 + Psyr_2822 0.46 +0.1 3,396,962 + Psyr_2822 0.53 +0.6 3,396,962 + Psyr_2822 0.53 -1.0 3,396,962 + Psyr_2822 0.53 -0.2 3,396,963 - Psyr_2822 0.53 +0.1 3,396,963 - Psyr_2822 0.53 +0.3 3,397,094 + Psyr_2822 0.89 -0.4 3,397,094 + Psyr_2822 0.89 -0.9 3,397,106 + -0.5 3,397,211 + -0.0 3,397,558 + Psyr_2823 0.90 -0.9 3,397,559 - -1.3 3,397,616 + +1.5 3,397,617 - +0.4 3,397,617 - +0.7 3,397,617 - -0.8 3,397,617 - +0.3 3,397,617 - +0.2 3,397,621 - -0.5 3,397,664 + +0.9 3,397,664 + -0.2 3,397,664 + +0.9 3,397,664 + +1.5 3,397,665 - +1.2 3,397,721 + +0.9 3,397,757 - -0.4
Or see this region's nucleotide sequence