Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2718 and Psyr_2719 are separated by 321 nucleotides Psyr_2719 and Psyr_2720 are separated by 5 nucleotides Psyr_2720 and Psyr_2721 overlap by 4 nucleotides
Psyr_2718: Psyr_2718 - Major royal jelly protein, at 3,300,251 to 3,301,435
_2718
Psyr_2719: Psyr_2719 - transcriptional regulator, TetR family, at 3,301,757 to 3,302,338
_2719
Psyr_2720: Psyr_2720 - Urea amidolyase-related protein, at 3,302,344 to 3,303,318
_2720
Psyr_2721: Psyr_2721 - Protein of unknown function DUF213, at 3,303,315 to 3,304,187
_2721
Position (kb)
3301
3302
3303 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3300.784 kb on + strand, within Psyr_2718 at 3300.785 kb on - strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.879 kb on + strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.880 kb on - strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.930 kb on + strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3300.931 kb on - strand, within Psyr_2718 at 3301.284 kb on + strand, within Psyr_2718 at 3301.285 kb on - strand, within Psyr_2718 at 3301.285 kb on - strand, within Psyr_2718 at 3301.285 kb on - strand, within Psyr_2718 at 3301.625 kb on - strand at 3301.699 kb on + strand at 3301.700 kb on - strand at 3301.700 kb on - strand at 3301.765 kb on + strand at 3301.766 kb on - strand at 3301.766 kb on - strand at 3301.838 kb on + strand, within Psyr_2719 at 3301.839 kb on - strand, within Psyr_2719 at 3301.899 kb on - strand, within Psyr_2719 at 3301.899 kb on - strand, within Psyr_2719 at 3302.006 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.009 kb on + strand, within Psyr_2719 at 3302.010 kb on - strand, within Psyr_2719 at 3302.010 kb on - strand, within Psyr_2719 at 3302.281 kb on - strand at 3302.387 kb on + strand at 3302.388 kb on - strand at 3302.388 kb on - strand at 3302.704 kb on + strand, within Psyr_2720 at 3302.760 kb on - strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.762 kb on + strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.763 kb on - strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.786 kb on + strand, within Psyr_2720 at 3302.787 kb on - strand, within Psyr_2720 at 3302.787 kb on - strand, within Psyr_2720 at 3302.892 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.927 kb on + strand, within Psyr_2720 at 3302.928 kb on - strand, within Psyr_2720 at 3302.928 kb on - strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.984 kb on + strand, within Psyr_2720 at 3302.985 kb on - strand, within Psyr_2720 at 3303.117 kb on + strand, within Psyr_2720 at 3303.118 kb on - strand, within Psyr_2720 at 3303.118 kb on - strand, within Psyr_2720 at 3303.216 kb on - strand, within Psyr_2720 at 3303.254 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 3,300,784 + Psyr_2718 0.45 -0.0 3,300,785 - Psyr_2718 0.45 -2.0 3,300,879 + Psyr_2718 0.53 +1.4 3,300,879 + Psyr_2718 0.53 -0.2 3,300,879 + Psyr_2718 0.53 -0.4 3,300,879 + Psyr_2718 0.53 -1.6 3,300,879 + Psyr_2718 0.53 -2.9 3,300,879 + Psyr_2718 0.53 +0.0 3,300,879 + Psyr_2718 0.53 -0.3 3,300,879 + Psyr_2718 0.53 +0.7 3,300,879 + Psyr_2718 0.53 -1.7 3,300,879 + Psyr_2718 0.53 +1.7 3,300,879 + Psyr_2718 0.53 +0.7 3,300,879 + Psyr_2718 0.53 +0.5 3,300,880 - Psyr_2718 0.53 +0.4 3,300,880 - Psyr_2718 0.53 +0.5 3,300,880 - Psyr_2718 0.53 -1.1 3,300,880 - Psyr_2718 0.53 -0.3 3,300,880 - Psyr_2718 0.53 -0.9 3,300,880 - Psyr_2718 0.53 +2.5 3,300,930 + Psyr_2718 0.57 +0.6 3,300,930 + Psyr_2718 0.57 -1.1 3,300,930 + Psyr_2718 0.57 +0.1 3,300,930 + Psyr_2718 0.57 +0.0 3,300,930 + Psyr_2718 0.57 -0.1 3,300,930 + Psyr_2718 0.57 -1.6 3,300,930 + Psyr_2718 0.57 +0.5 3,300,930 + Psyr_2718 0.57 +0.3 3,300,930 + Psyr_2718 0.57 -1.9 3,300,930 + Psyr_2718 0.57 -0.8 3,300,930 + Psyr_2718 0.57 -0.3 3,300,930 + Psyr_2718 0.57 -0.2 3,300,931 - Psyr_2718 0.57 +1.3 3,300,931 - Psyr_2718 0.57 -0.6 3,300,931 - Psyr_2718 0.57 -2.1 3,300,931 - Psyr_2718 0.57 +0.4 3,300,931 - Psyr_2718 0.57 -0.1 3,300,931 - Psyr_2718 0.57 +1.0 3,301,284 + Psyr_2718 0.87 -1.1 3,301,285 - Psyr_2718 0.87 -1.3 3,301,285 - Psyr_2718 0.87 -0.4 3,301,285 - Psyr_2718 0.87 -0.3 3,301,625 - -1.1 3,301,699 + -0.0 3,301,700 - +0.8 3,301,700 - -0.4 3,301,765 + +0.7 3,301,766 - -0.9 3,301,766 - +0.5 3,301,838 + Psyr_2719 0.14 -2.3 3,301,839 - Psyr_2719 0.14 +0.8 3,301,899 - Psyr_2719 0.24 -0.5 3,301,899 - Psyr_2719 0.24 -1.1 3,302,006 + Psyr_2719 0.43 -1.3 3,302,009 + Psyr_2719 0.43 -0.1 3,302,009 + Psyr_2719 0.43 +0.2 3,302,009 + Psyr_2719 0.43 -0.0 3,302,009 + Psyr_2719 0.43 -0.3 3,302,009 + Psyr_2719 0.43 -0.9 3,302,010 - Psyr_2719 0.43 -1.2 3,302,010 - Psyr_2719 0.43 +0.0 3,302,281 - -0.2 3,302,387 + -1.9 3,302,388 - -0.8 3,302,388 - -0.5 3,302,704 + Psyr_2720 0.37 -0.7 3,302,760 - Psyr_2720 0.43 -1.1 3,302,762 + Psyr_2720 0.43 +0.7 3,302,762 + Psyr_2720 0.43 +0.1 3,302,762 + Psyr_2720 0.43 -0.4 3,302,762 + Psyr_2720 0.43 -2.1 3,302,762 + Psyr_2720 0.43 -1.6 3,302,763 - Psyr_2720 0.43 +0.7 3,302,763 - Psyr_2720 0.43 +0.4 3,302,763 - Psyr_2720 0.43 -1.8 3,302,763 - Psyr_2720 0.43 +0.5 3,302,763 - Psyr_2720 0.43 +1.6 3,302,786 + Psyr_2720 0.45 +0.4 3,302,786 + Psyr_2720 0.45 +0.7 3,302,786 + Psyr_2720 0.45 +0.4 3,302,786 + Psyr_2720 0.45 +1.3 3,302,786 + Psyr_2720 0.45 -1.4 3,302,787 - Psyr_2720 0.45 -0.8 3,302,787 - Psyr_2720 0.45 -2.0 3,302,892 + Psyr_2720 0.56 -0.1 3,302,927 + Psyr_2720 0.60 -2.1 3,302,927 + Psyr_2720 0.60 +1.1 3,302,927 + Psyr_2720 0.60 +0.6 3,302,927 + Psyr_2720 0.60 +0.1 3,302,928 - Psyr_2720 0.60 +0.3 3,302,928 - Psyr_2720 0.60 -1.4 3,302,984 + Psyr_2720 0.66 -0.6 3,302,984 + Psyr_2720 0.66 -0.8 3,302,984 + Psyr_2720 0.66 -2.3 3,302,984 + Psyr_2720 0.66 -0.5 3,302,984 + Psyr_2720 0.66 +0.6 3,302,985 - Psyr_2720 0.66 -1.6 3,303,117 + Psyr_2720 0.79 +1.7 3,303,118 - Psyr_2720 0.79 -0.3 3,303,118 - Psyr_2720 0.79 +0.6 3,303,216 - Psyr_2720 0.89 +0.3 3,303,254 + +3.8
Or see this region's nucleotide sequence