Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2503 and Psyr_2504 are separated by 63 nucleotides Psyr_2504 and Psyr_2505 are separated by 9 nucleotides Psyr_2505 and Psyr_2506 overlap by 4 nucleotides Psyr_2506 and Psyr_2507 are separated by 16 nucleotides
Psyr_2503: Psyr_2503 - amino acid ABC transporter substrate-binding protein, PAAT family, at 2,913,035 to 2,913,886
_2503
Psyr_2504: Psyr_2504 - amino acid ABC transporter membrane protein 1, PAAT family, at 2,913,950 to 2,914,612
_2504
Psyr_2505: Psyr_2505 - amino acid ABC transporter membrane protein 2, PAAT family, at 2,914,622 to 2,915,284
_2505
Psyr_2506: Psyr_2506 - amino acid ABC transporter ATP-binding protein, PAAT family, at 2,915,281 to 2,916,066
_2506
Psyr_2507: Psyr_2507 - Endoribonuclease L-PSP, at 2,916,083 to 2,916,493
_2507
Position (kb)
2914
2915
2916 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2913.653 kb on + strand, within Psyr_2503 at 2913.654 kb on - strand, within Psyr_2503 at 2913.716 kb on + strand, within Psyr_2503 at 2913.716 kb on + strand, within Psyr_2503 at 2913.810 kb on - strand at 2913.878 kb on + strand at 2913.878 kb on + strand at 2913.879 kb on - strand at 2913.879 kb on - strand at 2913.879 kb on - strand at 2913.885 kb on - strand at 2913.885 kb on - strand at 2913.885 kb on - strand at 2914.098 kb on - strand, within Psyr_2504 at 2914.098 kb on - strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.178 kb on + strand, within Psyr_2504 at 2914.247 kb on + strand, within Psyr_2504 at 2914.259 kb on + strand, within Psyr_2504 at 2914.358 kb on + strand, within Psyr_2504 at 2914.358 kb on + strand, within Psyr_2504 at 2914.359 kb on - strand, within Psyr_2504 at 2914.359 kb on - strand, within Psyr_2504 at 2914.359 kb on - strand, within Psyr_2504 at 2914.361 kb on - strand, within Psyr_2504 at 2914.389 kb on + strand, within Psyr_2504 at 2914.389 kb on + strand, within Psyr_2504 at 2914.389 kb on + strand, within Psyr_2504 at 2914.390 kb on - strand, within Psyr_2504 at 2914.390 kb on - strand, within Psyr_2504 at 2914.390 kb on - strand, within Psyr_2504 at 2914.472 kb on + strand, within Psyr_2504 at 2914.472 kb on + strand, within Psyr_2504 at 2914.472 kb on + strand, within Psyr_2504 at 2914.473 kb on + strand, within Psyr_2504 at 2914.473 kb on - strand, within Psyr_2504 at 2914.473 kb on - strand, within Psyr_2504 at 2914.473 kb on - strand, within Psyr_2504 at 2914.473 kb on - strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.541 kb on + strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.542 kb on - strand, within Psyr_2504 at 2914.556 kb on + strand at 2914.625 kb on + strand at 2914.625 kb on + strand at 2914.625 kb on + strand at 2914.625 kb on + strand at 2914.625 kb on + strand at 2914.625 kb on + strand at 2914.626 kb on - strand at 2914.626 kb on - strand at 2914.626 kb on - strand at 2914.626 kb on - strand at 2914.626 kb on - strand at 2914.803 kb on - strand, within Psyr_2505 at 2914.803 kb on - strand, within Psyr_2505 at 2914.856 kb on + strand, within Psyr_2505 at 2914.856 kb on + strand, within Psyr_2505 at 2914.856 kb on + strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.857 kb on - strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2914.858 kb on + strand, within Psyr_2505 at 2915.034 kb on - strand, within Psyr_2505 at 2915.195 kb on + strand, within Psyr_2505 at 2915.195 kb on + strand, within Psyr_2505 at 2915.231 kb on + strand at 2915.231 kb on + strand at 2915.232 kb on - strand at 2915.232 kb on - strand at 2915.232 kb on - strand at 2915.232 kb on - strand at 2915.232 kb on - strand at 2915.335 kb on - strand at 2915.335 kb on - strand at 2915.335 kb on - strand at 2915.481 kb on + strand, within Psyr_2506 at 2915.482 kb on - strand, within Psyr_2506 at 2916.112 kb on + strand at 2916.112 kb on + strand at 2916.112 kb on + strand at 2916.113 kb on - strand at 2916.113 kb on - strand at 2916.115 kb on + strand at 2916.213 kb on + strand, within Psyr_2507 at 2916.213 kb on + strand, within Psyr_2507
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,913,653 + Psyr_2503 0.73 +0.5 2,913,654 - Psyr_2503 0.73 -0.0 2,913,716 + Psyr_2503 0.80 +0.1 2,913,716 + Psyr_2503 0.80 -2.6 2,913,810 - -1.1 2,913,878 + -1.2 2,913,878 + -0.2 2,913,879 - +0.1 2,913,879 - -1.3 2,913,879 - +0.2 2,913,885 - +0.3 2,913,885 - -3.2 2,913,885 - -0.1 2,914,098 - Psyr_2504 0.22 -1.0 2,914,098 - Psyr_2504 0.22 +2.8 2,914,178 + Psyr_2504 0.34 -1.9 2,914,178 + Psyr_2504 0.34 -1.8 2,914,178 + Psyr_2504 0.34 +0.7 2,914,178 + Psyr_2504 0.34 -0.6 2,914,178 + Psyr_2504 0.34 -1.3 2,914,178 + Psyr_2504 0.34 -1.1 2,914,247 + Psyr_2504 0.45 -0.7 2,914,259 + Psyr_2504 0.47 -2.3 2,914,358 + Psyr_2504 0.62 -2.7 2,914,358 + Psyr_2504 0.62 +0.7 2,914,359 - Psyr_2504 0.62 -0.8 2,914,359 - Psyr_2504 0.62 +1.3 2,914,359 - Psyr_2504 0.62 -1.7 2,914,361 - Psyr_2504 0.62 -1.4 2,914,389 + Psyr_2504 0.66 +0.9 2,914,389 + Psyr_2504 0.66 -0.4 2,914,389 + Psyr_2504 0.66 -2.1 2,914,390 - Psyr_2504 0.66 -1.1 2,914,390 - Psyr_2504 0.66 -0.9 2,914,390 - Psyr_2504 0.66 -1.4 2,914,472 + Psyr_2504 0.79 -0.5 2,914,472 + Psyr_2504 0.79 -1.3 2,914,472 + Psyr_2504 0.79 -1.0 2,914,473 + Psyr_2504 0.79 -2.0 2,914,473 - Psyr_2504 0.79 -0.1 2,914,473 - Psyr_2504 0.79 +0.7 2,914,473 - Psyr_2504 0.79 +0.7 2,914,473 - Psyr_2504 0.79 -1.2 2,914,541 + Psyr_2504 0.89 -2.5 2,914,541 + Psyr_2504 0.89 -0.1 2,914,541 + Psyr_2504 0.89 +0.3 2,914,541 + Psyr_2504 0.89 -0.8 2,914,541 + Psyr_2504 0.89 -0.6 2,914,541 + Psyr_2504 0.89 -1.6 2,914,541 + Psyr_2504 0.89 -1.9 2,914,541 + Psyr_2504 0.89 -1.9 2,914,542 - Psyr_2504 0.89 -0.8 2,914,542 - Psyr_2504 0.89 +0.1 2,914,542 - Psyr_2504 0.89 -0.3 2,914,542 - Psyr_2504 0.89 -0.4 2,914,542 - Psyr_2504 0.89 +0.1 2,914,542 - Psyr_2504 0.89 -0.4 2,914,542 - Psyr_2504 0.89 +1.1 2,914,542 - Psyr_2504 0.89 +0.7 2,914,542 - Psyr_2504 0.89 -1.3 2,914,542 - Psyr_2504 0.89 -0.3 2,914,542 - Psyr_2504 0.89 +0.3 2,914,542 - Psyr_2504 0.89 +0.4 2,914,542 - Psyr_2504 0.89 +0.1 2,914,556 + -0.9 2,914,625 + +0.7 2,914,625 + -0.7 2,914,625 + +2.6 2,914,625 + +1.2 2,914,625 + -0.1 2,914,625 + -1.0 2,914,626 - +0.4 2,914,626 - -2.5 2,914,626 - +0.6 2,914,626 - +1.4 2,914,626 - -1.0 2,914,803 - Psyr_2505 0.27 +0.6 2,914,803 - Psyr_2505 0.27 -0.2 2,914,856 + Psyr_2505 0.35 -1.5 2,914,856 + Psyr_2505 0.35 -0.9 2,914,856 + Psyr_2505 0.35 -0.6 2,914,857 - Psyr_2505 0.35 -2.1 2,914,857 - Psyr_2505 0.35 -0.8 2,914,857 - Psyr_2505 0.35 -0.5 2,914,857 - Psyr_2505 0.35 +1.8 2,914,857 - Psyr_2505 0.35 -1.7 2,914,857 - Psyr_2505 0.35 -0.7 2,914,857 - Psyr_2505 0.35 -0.0 2,914,857 - Psyr_2505 0.35 +0.7 2,914,858 + Psyr_2505 0.36 -2.5 2,914,858 + Psyr_2505 0.36 -0.6 2,914,858 + Psyr_2505 0.36 -0.4 2,914,858 + Psyr_2505 0.36 -0.0 2,914,858 + Psyr_2505 0.36 -0.9 2,914,858 + Psyr_2505 0.36 -0.9 2,915,034 - Psyr_2505 0.62 +1.4 2,915,195 + Psyr_2505 0.86 -0.0 2,915,195 + Psyr_2505 0.86 -1.6 2,915,231 + -1.8 2,915,231 + -2.2 2,915,232 - +0.7 2,915,232 - -1.0 2,915,232 - +0.2 2,915,232 - +0.0 2,915,232 - +0.8 2,915,335 - +0.0 2,915,335 - +0.9 2,915,335 - +1.3 2,915,481 + Psyr_2506 0.25 -3.1 2,915,482 - Psyr_2506 0.26 -3.0 2,916,112 + +0.5 2,916,112 + -0.5 2,916,112 + -0.8 2,916,113 - +0.0 2,916,113 - +1.6 2,916,115 + +0.0 2,916,213 + Psyr_2507 0.32 -1.5 2,916,213 + Psyr_2507 0.32 -0.7
Or see this region's nucleotide sequence