Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2407 and Psyr_2408 overlap by 4 nucleotides Psyr_2408 and Psyr_2409 are separated by 34 nucleotides Psyr_2409 and Psyr_2410 are separated by 270 nucleotides
Psyr_2407: Psyr_2407 - aldehyde dehydrogenase (acceptor), at 2,787,944 to 2,789,425
_2407
Psyr_2408: Psyr_2408 - 3-oxoadipate enol-lactonase, at 2,789,422 to 2,790,270
_2408
Psyr_2409: Psyr_2409 - Protein of unknown function DUF1185, at 2,790,305 to 2,790,895
_2409
Psyr_2410: Psyr_2410 - Zinc-containing alcohol dehydrogenase superfamily, at 2,791,166 to 2,792,305
_2410
Position (kb)
2789
2790
2791 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2788.530 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.590 kb on + strand, within Psyr_2407 at 2788.591 kb on - strand, within Psyr_2407 at 2788.749 kb on - strand, within Psyr_2407 at 2788.953 kb on + strand, within Psyr_2407 at 2789.013 kb on + strand, within Psyr_2407 at 2789.014 kb on - strand, within Psyr_2407 at 2789.083 kb on - strand, within Psyr_2407 at 2789.539 kb on - strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.684 kb on + strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.685 kb on - strand, within Psyr_2408 at 2789.688 kb on + strand, within Psyr_2408 at 2789.689 kb on - strand, within Psyr_2408 at 2789.689 kb on - strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.711 kb on + strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2789.712 kb on - strand, within Psyr_2408 at 2790.129 kb on - strand, within Psyr_2408 at 2790.228 kb on - strand at 2790.228 kb on - strand at 2790.346 kb on - strand at 2790.453 kb on + strand, within Psyr_2409 at 2790.453 kb on + strand, within Psyr_2409 at 2790.453 kb on + strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.454 kb on - strand, within Psyr_2409 at 2790.508 kb on + strand, within Psyr_2409 at 2790.509 kb on - strand, within Psyr_2409 at 2790.543 kb on + strand, within Psyr_2409 at 2790.544 kb on - strand, within Psyr_2409 at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.864 kb on + strand at 2790.916 kb on - strand at 2790.968 kb on + strand at 2790.969 kb on - strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.006 kb on + strand at 2791.007 kb on - strand at 2791.018 kb on + strand at 2791.111 kb on + strand at 2791.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,788,530 + Psyr_2407 0.40 +0.3 2,788,590 + Psyr_2407 0.44 +1.3 2,788,590 + Psyr_2407 0.44 -1.5 2,788,591 - Psyr_2407 0.44 +0.6 2,788,749 - Psyr_2407 0.54 +0.9 2,788,953 + Psyr_2407 0.68 -1.0 2,789,013 + Psyr_2407 0.72 +1.2 2,789,014 - Psyr_2407 0.72 -0.3 2,789,083 - Psyr_2407 0.77 -0.5 2,789,539 - Psyr_2408 0.14 -0.6 2,789,684 + Psyr_2408 0.31 -1.3 2,789,684 + Psyr_2408 0.31 +0.1 2,789,684 + Psyr_2408 0.31 +0.1 2,789,685 - Psyr_2408 0.31 -0.7 2,789,685 - Psyr_2408 0.31 -0.2 2,789,685 - Psyr_2408 0.31 -0.4 2,789,688 + Psyr_2408 0.31 -0.6 2,789,689 - Psyr_2408 0.31 -2.5 2,789,689 - Psyr_2408 0.31 +0.3 2,789,711 + Psyr_2408 0.34 +1.2 2,789,711 + Psyr_2408 0.34 +1.8 2,789,711 + Psyr_2408 0.34 -1.9 2,789,711 + Psyr_2408 0.34 +0.3 2,789,712 - Psyr_2408 0.34 -1.2 2,789,712 - Psyr_2408 0.34 -0.3 2,789,712 - Psyr_2408 0.34 +3.4 2,790,129 - Psyr_2408 0.83 -0.9 2,790,228 - -0.5 2,790,228 - +1.5 2,790,346 - -1.9 2,790,453 + Psyr_2409 0.25 -0.8 2,790,453 + Psyr_2409 0.25 -2.0 2,790,453 + Psyr_2409 0.25 +0.9 2,790,454 - Psyr_2409 0.25 +0.6 2,790,454 - Psyr_2409 0.25 -0.1 2,790,454 - Psyr_2409 0.25 -1.3 2,790,508 + Psyr_2409 0.34 -1.8 2,790,509 - Psyr_2409 0.35 -0.3 2,790,543 + Psyr_2409 0.40 -2.2 2,790,544 - Psyr_2409 0.40 -0.8 2,790,864 + +0.3 2,790,864 + -0.9 2,790,864 + +0.9 2,790,864 + -1.6 2,790,916 - -0.2 2,790,968 + -3.0 2,790,969 - -0.7 2,791,006 + +0.0 2,791,006 + +1.1 2,791,006 + +0.3 2,791,006 + -0.0 2,791,007 - -0.5 2,791,018 + -1.5 2,791,111 + +0.0 2,791,182 - +0.3
Or see this region's nucleotide sequence