Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2001 and Psyr_2002 are separated by 94 nucleotides Psyr_2002 and Psyr_2003 are separated by 74 nucleotides Psyr_2003 and Psyr_2004 are separated by 66 nucleotides
Psyr_2001: Psyr_2001 - conserved hypothetical protein, at 2,322,793 to 2,323,434
_2001
Psyr_2002: Psyr_2002 - Protein of unknown function UPF0153, at 2,323,529 to 2,323,786
_2002
Psyr_2003: Psyr_2003 - conserved hypothetical protein, at 2,323,861 to 2,324,454
_2003
Psyr_2004: Psyr_2004 - citrate synthase, at 2,324,521 to 2,325,810
_2004
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2323.257 kb on + strand, within Psyr_2001 at 2323.377 kb on + strand at 2323.377 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.397 kb on + strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.398 kb on - strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.432 kb on + strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.433 kb on - strand at 2323.563 kb on + strand, within Psyr_2002 at 2323.856 kb on - strand at 2323.886 kb on + strand at 2323.886 kb on + strand at 2323.887 kb on - strand at 2323.887 kb on - strand at 2323.981 kb on + strand, within Psyr_2003 at 2323.981 kb on + strand, within Psyr_2003 at 2323.982 kb on - strand, within Psyr_2003 at 2323.990 kb on + strand, within Psyr_2003 at 2323.991 kb on - strand, within Psyr_2003 at 2323.999 kb on - strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.021 kb on + strand, within Psyr_2003 at 2324.128 kb on + strand, within Psyr_2003 at 2324.128 kb on + strand, within Psyr_2003 at 2324.129 kb on - strand, within Psyr_2003 at 2324.142 kb on + strand, within Psyr_2003 at 2324.143 kb on - strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.242 kb on + strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.243 kb on - strand, within Psyr_2003 at 2324.263 kb on + strand, within Psyr_2003 at 2324.263 kb on + strand, within Psyr_2003 at 2324.264 kb on - strand, within Psyr_2003 at 2324.264 kb on - strand, within Psyr_2003 at 2324.335 kb on + strand, within Psyr_2003 at 2324.412 kb on + strand at 2324.412 kb on + strand at 2324.412 kb on + strand at 2324.413 kb on - strand at 2324.475 kb on + strand at 2324.522 kb on + strand at 2324.523 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 2,323,257 + Psyr_2001 0.72 +0.1 2,323,377 + -2.4 2,323,377 + +0.2 2,323,397 + +0.1 2,323,397 + -0.4 2,323,397 + +1.6 2,323,397 + +0.6 2,323,397 + +1.3 2,323,397 + -1.1 2,323,398 - +1.3 2,323,398 - -1.2 2,323,398 - -0.4 2,323,398 - -0.5 2,323,432 + -0.3 2,323,432 + -0.9 2,323,432 + +0.4 2,323,432 + +0.5 2,323,433 - +0.0 2,323,433 - -0.2 2,323,433 - -1.0 2,323,433 - -2.1 2,323,433 - +0.4 2,323,433 - +0.6 2,323,563 + Psyr_2002 0.13 -2.2 2,323,856 - +0.8 2,323,886 + -1.4 2,323,886 + +1.3 2,323,887 - -1.2 2,323,887 - +1.2 2,323,981 + Psyr_2003 0.20 -0.2 2,323,981 + Psyr_2003 0.20 +1.7 2,323,982 - Psyr_2003 0.20 +1.5 2,323,990 + Psyr_2003 0.22 +0.2 2,323,991 - Psyr_2003 0.22 +0.5 2,323,999 - Psyr_2003 0.23 -1.9 2,324,021 + Psyr_2003 0.27 -0.2 2,324,021 + Psyr_2003 0.27 +0.1 2,324,021 + Psyr_2003 0.27 +0.6 2,324,128 + Psyr_2003 0.45 +0.2 2,324,128 + Psyr_2003 0.45 +0.2 2,324,129 - Psyr_2003 0.45 +1.1 2,324,142 + Psyr_2003 0.47 -1.2 2,324,143 - Psyr_2003 0.47 +1.1 2,324,242 + Psyr_2003 0.64 +0.9 2,324,242 + Psyr_2003 0.64 -0.3 2,324,242 + Psyr_2003 0.64 +0.1 2,324,242 + Psyr_2003 0.64 +1.6 2,324,242 + Psyr_2003 0.64 +0.2 2,324,242 + Psyr_2003 0.64 -0.1 2,324,242 + Psyr_2003 0.64 +0.1 2,324,242 + Psyr_2003 0.64 -0.5 2,324,242 + Psyr_2003 0.64 +1.0 2,324,242 + Psyr_2003 0.64 +1.0 2,324,242 + Psyr_2003 0.64 +0.5 2,324,243 - Psyr_2003 0.64 -1.4 2,324,243 - Psyr_2003 0.64 -0.5 2,324,243 - Psyr_2003 0.64 +0.8 2,324,243 - Psyr_2003 0.64 -0.3 2,324,243 - Psyr_2003 0.64 -1.4 2,324,243 - Psyr_2003 0.64 +1.2 2,324,243 - Psyr_2003 0.64 -0.1 2,324,243 - Psyr_2003 0.64 +1.3 2,324,243 - Psyr_2003 0.64 -0.1 2,324,243 - Psyr_2003 0.64 +1.9 2,324,243 - Psyr_2003 0.64 +1.7 2,324,263 + Psyr_2003 0.68 -0.1 2,324,263 + Psyr_2003 0.68 +0.8 2,324,264 - Psyr_2003 0.68 -2.0 2,324,264 - Psyr_2003 0.68 -0.9 2,324,335 + Psyr_2003 0.80 +0.3 2,324,412 + +0.2 2,324,412 + -0.3 2,324,412 + +2.5 2,324,413 - -0.7 2,324,475 + +0.1 2,324,522 + +0.2 2,324,523 - +1.8
Or see this region's nucleotide sequence