Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1102 and Psyr_1103 are separated by 98 nucleotides Psyr_1103 and Psyr_1104 overlap by 4 nucleotides Psyr_1104 and Psyr_1105 are separated by 116 nucleotides
Psyr_1102: Psyr_1102 - conserved hypothetical protein, at 1,248,790 to 1,249,146
_1102
Psyr_1103: Psyr_1103 - Protein of unknown function DUF454, at 1,249,245 to 1,249,703
_1103
Psyr_1104: Psyr_1104 - Heme oxygenase, at 1,249,700 to 1,250,314
_1104
Psyr_1105: Psyr_1105 - TonB-dependent hemoglobin/transferrin/lactoferrin receptor:TonB-dependent heme/hemoglobin receptor, at 1,250,431 to 1,253,010
_1105
Position (kb)
1249
1250 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1248.268 kb on - strand at 1248.268 kb on - strand at 1248.268 kb on - strand at 1248.458 kb on - strand at 1248.458 kb on - strand at 1248.458 kb on - strand at 1248.561 kb on - strand at 1248.648 kb on - strand at 1248.750 kb on + strand at 1248.751 kb on - strand at 1248.751 kb on - strand at 1248.791 kb on + strand at 1249.067 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.085 kb on + strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.086 kb on - strand, within Psyr_1102 at 1249.142 kb on + strand at 1249.142 kb on + strand at 1249.142 kb on + strand at 1249.142 kb on + strand at 1249.142 kb on + strand at 1249.143 kb on - strand at 1249.143 kb on - strand at 1249.254 kb on + strand at 1249.254 kb on + strand at 1249.255 kb on - strand at 1249.255 kb on - strand at 1249.255 kb on - strand at 1249.287 kb on + strand at 1249.287 kb on + strand at 1249.287 kb on + strand at 1249.288 kb on - strand at 1249.288 kb on - strand at 1249.321 kb on + strand, within Psyr_1103 at 1249.321 kb on + strand, within Psyr_1103 at 1249.322 kb on - strand, within Psyr_1103 at 1249.322 kb on - strand, within Psyr_1103 at 1249.435 kb on + strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.436 kb on - strand, within Psyr_1103 at 1249.569 kb on + strand, within Psyr_1103 at 1249.569 kb on + strand, within Psyr_1103 at 1249.570 kb on - strand, within Psyr_1103 at 1249.603 kb on - strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.636 kb on + strand, within Psyr_1103 at 1249.637 kb on - strand, within Psyr_1103 at 1249.637 kb on - strand, within Psyr_1103 at 1249.637 kb on - strand, within Psyr_1103 at 1249.650 kb on + strand, within Psyr_1103 at 1249.681 kb on - strand at 1250.136 kb on + strand, within Psyr_1104 at 1250.137 kb on - strand, within Psyr_1104 at 1250.167 kb on - strand, within Psyr_1104 at 1250.167 kb on - strand, within Psyr_1104 at 1250.191 kb on - strand, within Psyr_1104 at 1250.317 kb on + strand at 1250.318 kb on - strand at 1250.318 kb on - strand at 1250.349 kb on + strand at 1250.349 kb on + strand at 1250.350 kb on - strand at 1250.350 kb on - strand at 1250.559 kb on - strand at 1250.579 kb on + strand at 1250.579 kb on + strand at 1250.580 kb on - strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.618 kb on + strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand at 1250.619 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 1,248,268 - +0.4 1,248,268 - -0.8 1,248,268 - -0.7 1,248,458 - +0.4 1,248,458 - -0.0 1,248,458 - +0.5 1,248,561 - +0.4 1,248,648 - +0.4 1,248,750 + -1.3 1,248,751 - +0.9 1,248,751 - -1.2 1,248,791 + +0.6 1,249,067 + Psyr_1102 0.78 +0.3 1,249,085 + Psyr_1102 0.83 +0.9 1,249,085 + Psyr_1102 0.83 +2.0 1,249,085 + Psyr_1102 0.83 +0.3 1,249,085 + Psyr_1102 0.83 -0.8 1,249,085 + Psyr_1102 0.83 +0.1 1,249,085 + Psyr_1102 0.83 +0.7 1,249,085 + Psyr_1102 0.83 +0.1 1,249,086 - Psyr_1102 0.83 +1.1 1,249,086 - Psyr_1102 0.83 -0.4 1,249,086 - Psyr_1102 0.83 -1.5 1,249,086 - Psyr_1102 0.83 +2.3 1,249,086 - Psyr_1102 0.83 +0.7 1,249,086 - Psyr_1102 0.83 +0.3 1,249,086 - Psyr_1102 0.83 +0.5 1,249,086 - Psyr_1102 0.83 -0.1 1,249,142 + +0.5 1,249,142 + -0.9 1,249,142 + -0.7 1,249,142 + +1.2 1,249,142 + -1.1 1,249,143 - -1.1 1,249,143 - +5.2 1,249,254 + -0.6 1,249,254 + -0.1 1,249,255 - +0.1 1,249,255 - -1.9 1,249,255 - -0.1 1,249,287 + +1.2 1,249,287 + -2.1 1,249,287 + -1.1 1,249,288 - +0.7 1,249,288 - +0.9 1,249,321 + Psyr_1103 0.17 -0.3 1,249,321 + Psyr_1103 0.17 -0.6 1,249,322 - Psyr_1103 0.17 -0.1 1,249,322 - Psyr_1103 0.17 +0.8 1,249,435 + Psyr_1103 0.41 -1.5 1,249,436 - Psyr_1103 0.42 -1.4 1,249,436 - Psyr_1103 0.42 -1.2 1,249,436 - Psyr_1103 0.42 -1.2 1,249,436 - Psyr_1103 0.42 -1.2 1,249,436 - Psyr_1103 0.42 -0.8 1,249,569 + Psyr_1103 0.71 -0.7 1,249,569 + Psyr_1103 0.71 +0.3 1,249,570 - Psyr_1103 0.71 -2.1 1,249,603 - Psyr_1103 0.78 +0.9 1,249,636 + Psyr_1103 0.85 -0.6 1,249,636 + Psyr_1103 0.85 +1.1 1,249,636 + Psyr_1103 0.85 +1.5 1,249,636 + Psyr_1103 0.85 +0.2 1,249,636 + Psyr_1103 0.85 -1.7 1,249,637 - Psyr_1103 0.85 +0.4 1,249,637 - Psyr_1103 0.85 -0.7 1,249,637 - Psyr_1103 0.85 -0.7 1,249,650 + Psyr_1103 0.88 +1.1 1,249,681 - -0.5 1,250,136 + Psyr_1104 0.71 -1.0 1,250,137 - Psyr_1104 0.71 +0.1 1,250,167 - Psyr_1104 0.76 -0.3 1,250,167 - Psyr_1104 0.76 -1.1 1,250,191 - Psyr_1104 0.80 +0.1 1,250,317 + -1.0 1,250,318 - -0.3 1,250,318 - -0.9 1,250,349 + -1.7 1,250,349 + +1.6 1,250,350 - +0.1 1,250,350 - -0.5 1,250,559 - -3.0 1,250,579 + -1.7 1,250,579 + -0.4 1,250,580 - -1.6 1,250,618 + +0.8 1,250,618 + -0.2 1,250,618 + +0.8 1,250,618 + +0.0 1,250,618 + +1.2 1,250,618 + -0.8 1,250,618 + +0.0 1,250,618 + -1.9 1,250,618 + -0.8 1,250,619 - -0.8 1,250,619 - +0.1 1,250,619 - -1.0 1,250,619 - +0.4 1,250,619 - -0.2
Or see this region's nucleotide sequence