Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0216 and Psyr_0217 are separated by 10 nucleotides Psyr_0217 and Psyr_0218 are separated by 331 nucleotides Psyr_0218 and Psyr_0219 are separated by 28 nucleotides
Psyr_0216: Psyr_0216 - orotate phosphoribosyltransferase, at 229,459 to 230,100
_0216
Psyr_0217: Psyr_0217 - conserved hypothetical protein, at 230,111 to 230,743
_0217
Psyr_0218: Psyr_0218 - N-acetylglutamate kinase, at 231,075 to 231,980
_0218
Psyr_0219: Psyr_0219 - phosphomannomutase, at 232,009 to 233,406
_0219
Position (kb)
231
232 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 230.238 kb on - strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.297 kb on + strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.298 kb on - strand, within Psyr_0217 at 230.313 kb on - strand, within Psyr_0217 at 230.365 kb on + strand, within Psyr_0217 at 230.438 kb on + strand, within Psyr_0217 at 230.439 kb on - strand, within Psyr_0217 at 230.839 kb on + strand at 231.043 kb on - strand at 231.065 kb on + strand at 231.066 kb on - strand at 231.109 kb on - strand at 231.109 kb on - strand at 231.920 kb on - strand at 232.291 kb on - strand, within Psyr_0219 at 232.291 kb on - strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.370 kb on + strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.371 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.375 kb on - strand, within Psyr_0219 at 232.925 kb on + strand, within Psyr_0219 at 232.926 kb on - strand, within Psyr_0219
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 230,238 - Psyr_0217 0.20 -1.4 230,297 + Psyr_0217 0.29 +1.4 230,297 + Psyr_0217 0.29 +0.9 230,298 - Psyr_0217 0.30 -0.9 230,298 - Psyr_0217 0.30 -0.4 230,298 - Psyr_0217 0.30 -1.0 230,298 - Psyr_0217 0.30 +0.8 230,313 - Psyr_0217 0.32 +1.7 230,365 + Psyr_0217 0.40 -0.1 230,438 + Psyr_0217 0.52 +0.5 230,439 - Psyr_0217 0.52 -1.1 230,839 + +0.5 231,043 - +0.8 231,065 + -0.3 231,066 - +0.6 231,109 - -0.9 231,109 - -1.3 231,920 - +3.2 232,291 - Psyr_0219 0.20 +0.2 232,291 - Psyr_0219 0.20 +0.4 232,370 + Psyr_0219 0.26 +0.5 232,370 + Psyr_0219 0.26 -1.1 232,370 + Psyr_0219 0.26 -0.7 232,370 + Psyr_0219 0.26 -0.4 232,371 - Psyr_0219 0.26 +1.5 232,371 - Psyr_0219 0.26 -0.7 232,371 - Psyr_0219 0.26 -1.8 232,371 - Psyr_0219 0.26 -1.2 232,371 - Psyr_0219 0.26 +3.4 232,371 - Psyr_0219 0.26 +0.9 232,371 - Psyr_0219 0.26 +0.9 232,371 - Psyr_0219 0.26 +1.1 232,371 - Psyr_0219 0.26 -2.6 232,375 - Psyr_0219 0.26 -0.1 232,375 - Psyr_0219 0.26 -0.0 232,375 - Psyr_0219 0.26 +0.7 232,925 + Psyr_0219 0.66 +1.5 232,926 - Psyr_0219 0.66 -0.8
Or see this region's nucleotide sequence