Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0203 and Psyr_0204 overlap by 4 nucleotides Psyr_0204 and Psyr_0205 are separated by 3 nucleotides Psyr_0205 and Psyr_0206 are separated by 208 nucleotides
Psyr_0203: Psyr_0203 - outer membrane transport energization protein TonB, at 218,563 to 219,309
_0203
Psyr_0204: Psyr_0204 - outer membrane transport energization protein ExbD, at 219,306 to 219,731
_0204
Psyr_0205: Psyr_0205 - outer membrane transport energization protein ExbB, at 219,735 to 220,697
_0205
Psyr_0206: Psyr_0206 - conserved hypothetical protein, at 220,906 to 221,754
_0206
Position (kb) 219
220
221 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 219.279 kb on - strand at 220.814 kb on - strand at 220.814 kb on - strand at 220.816 kb on + strand at 220.816 kb on + strand at 220.817 kb on - strand at 220.817 kb on - strand at 220.817 kb on - strand at 220.831 kb on - strand at 220.834 kb on + strand at 220.834 kb on + strand at 220.834 kb on + strand at 220.834 kb on + strand at 220.835 kb on - strand at 220.835 kb on - strand at 221.101 kb on + strand, within Psyr_0206 at 221.101 kb on + strand, within Psyr_0206 at 221.101 kb on + strand, within Psyr_0206 at 221.101 kb on + strand, within Psyr_0206 at 221.102 kb on - strand, within Psyr_0206 at 221.102 kb on - strand, within Psyr_0206 at 221.102 kb on - strand, within Psyr_0206 at 221.102 kb on - strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.110 kb on + strand, within Psyr_0206 at 221.111 kb on - strand, within Psyr_0206 at 221.111 kb on - strand, within Psyr_0206 at 221.111 kb on - strand, within Psyr_0206 at 221.111 kb on - strand, within Psyr_0206 at 221.111 kb on - strand, within Psyr_0206 at 221.158 kb on + strand, within Psyr_0206 at 221.158 kb on + strand, within Psyr_0206 at 221.158 kb on + strand, within Psyr_0206 at 221.158 kb on + strand, within Psyr_0206 at 221.158 kb on + strand, within Psyr_0206 at 221.159 kb on - strand, within Psyr_0206 at 221.159 kb on - strand, within Psyr_0206 at 221.159 kb on - strand, within Psyr_0206 at 221.175 kb on + strand, within Psyr_0206 at 221.176 kb on - strand, within Psyr_0206 at 221.305 kb on + strand, within Psyr_0206 at 221.305 kb on + strand, within Psyr_0206 at 221.305 kb on + strand, within Psyr_0206 at 221.306 kb on - strand, within Psyr_0206 at 221.306 kb on - strand, within Psyr_0206 at 221.306 kb on - strand, within Psyr_0206 at 221.306 kb on - strand, within Psyr_0206 at 221.306 kb on - strand, within Psyr_0206 at 221.389 kb on + strand, within Psyr_0206 at 221.472 kb on + strand, within Psyr_0206 at 221.473 kb on - strand, within Psyr_0206 at 221.552 kb on - strand, within Psyr_0206 at 221.611 kb on + strand, within Psyr_0206 at 221.611 kb on + strand, within Psyr_0206 at 221.612 kb on - strand, within Psyr_0206 at 221.612 kb on - strand, within Psyr_0206 at 221.612 kb on - strand, within Psyr_0206
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 219,279 - +0.1 220,814 - +0.9 220,814 - -3.0 220,816 + -1.5 220,816 + -1.2 220,817 - +0.5 220,817 - -2.4 220,817 - -0.4 220,831 - +1.0 220,834 + -1.8 220,834 + -0.2 220,834 + -2.2 220,834 + -1.1 220,835 - +0.6 220,835 - -1.1 221,101 + Psyr_0206 0.23 -0.2 221,101 + Psyr_0206 0.23 -0.2 221,101 + Psyr_0206 0.23 +1.1 221,101 + Psyr_0206 0.23 -1.0 221,102 - Psyr_0206 0.23 -0.0 221,102 - Psyr_0206 0.23 +1.0 221,102 - Psyr_0206 0.23 +0.8 221,102 - Psyr_0206 0.23 +1.1 221,110 + Psyr_0206 0.24 -0.7 221,110 + Psyr_0206 0.24 -0.6 221,110 + Psyr_0206 0.24 +0.8 221,110 + Psyr_0206 0.24 +3.0 221,110 + Psyr_0206 0.24 -1.7 221,110 + Psyr_0206 0.24 -0.9 221,110 + Psyr_0206 0.24 +0.5 221,111 - Psyr_0206 0.24 -0.3 221,111 - Psyr_0206 0.24 +1.3 221,111 - Psyr_0206 0.24 -0.4 221,111 - Psyr_0206 0.24 -1.7 221,111 - Psyr_0206 0.24 +2.5 221,158 + Psyr_0206 0.30 +0.5 221,158 + Psyr_0206 0.30 +0.3 221,158 + Psyr_0206 0.30 -0.6 221,158 + Psyr_0206 0.30 -2.4 221,158 + Psyr_0206 0.30 -0.8 221,159 - Psyr_0206 0.30 -1.7 221,159 - Psyr_0206 0.30 -0.6 221,159 - Psyr_0206 0.30 -0.5 221,175 + Psyr_0206 0.32 +2.5 221,176 - Psyr_0206 0.32 +0.2 221,305 + Psyr_0206 0.47 +1.7 221,305 + Psyr_0206 0.47 +0.0 221,305 + Psyr_0206 0.47 +0.7 221,306 - Psyr_0206 0.47 -0.5 221,306 - Psyr_0206 0.47 +1.7 221,306 - Psyr_0206 0.47 +0.3 221,306 - Psyr_0206 0.47 +0.1 221,306 - Psyr_0206 0.47 -0.5 221,389 + Psyr_0206 0.57 -1.8 221,472 + Psyr_0206 0.67 +0.9 221,473 - Psyr_0206 0.67 -0.8 221,552 - Psyr_0206 0.76 -1.2 221,611 + Psyr_0206 0.83 -0.2 221,611 + Psyr_0206 0.83 +1.2 221,612 - Psyr_0206 0.83 -3.0 221,612 - Psyr_0206 0.83 -0.5 221,612 - Psyr_0206 0.83 -1.4
Or see this region's nucleotide sequence