Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0198 and Psyr_0199 are separated by 296 nucleotides Psyr_0199 and Psyr_0200 are separated by 62 nucleotides Psyr_0200 and Psyr_0201 are separated by 86 nucleotides
Psyr_0198: Psyr_0198 - Citrate transporter, at 211,908 to 213,215
_0198
Psyr_0199: Psyr_0199 - Glutathione-dependent formaldehyde-activating, GFA, at 213,512 to 213,913
_0199
Psyr_0200: Psyr_0200 - conserved hypothetical protein, at 213,976 to 215,373
_0200
Psyr_0201: Psyr_0201 - ATP-dependent DNA helicase RecG, at 215,460 to 217,535
_0201
Position (kb) 213
214
215
216 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 213.010 kb on + strand, within Psyr_0198 at 213.010 kb on + strand, within Psyr_0198 at 213.059 kb on + strand, within Psyr_0198 at 213.060 kb on - strand, within Psyr_0198 at 213.060 kb on - strand, within Psyr_0198 at 213.172 kb on + strand at 213.172 kb on + strand at 213.172 kb on + strand at 213.172 kb on + strand at 213.172 kb on + strand at 213.172 kb on + strand at 213.173 kb on - strand at 213.173 kb on - strand at 213.173 kb on - strand at 213.173 kb on - strand at 213.173 kb on - strand at 213.185 kb on + strand at 213.232 kb on + strand at 213.232 kb on + strand at 213.232 kb on + strand at 213.232 kb on + strand at 213.233 kb on - strand at 213.233 kb on - strand at 213.233 kb on - strand at 213.233 kb on - strand at 213.235 kb on + strand at 213.235 kb on + strand at 213.236 kb on - strand at 213.236 kb on - strand at 213.281 kb on - strand at 213.343 kb on + strand at 213.343 kb on + strand at 213.344 kb on - strand at 213.344 kb on - strand at 213.352 kb on + strand at 213.352 kb on + strand at 213.352 kb on + strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.353 kb on - strand at 213.422 kb on + strand at 213.423 kb on - strand at 213.423 kb on - strand at 213.423 kb on - strand at 213.423 kb on - strand at 213.423 kb on - strand at 213.472 kb on - strand at 213.696 kb on - strand, within Psyr_0199 at 213.722 kb on + strand, within Psyr_0199 at 213.722 kb on + strand, within Psyr_0199 at 213.847 kb on - strand, within Psyr_0199 at 213.959 kb on + strand at 213.959 kb on + strand at 213.959 kb on + strand at 213.959 kb on + strand at 213.959 kb on + strand at 213.959 kb on + strand at 213.959 kb on + strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.960 kb on - strand at 213.964 kb on + strand at 213.964 kb on + strand at 213.964 kb on + strand at 213.964 kb on + strand at 213.964 kb on + strand at 213.964 kb on + strand at 213.965 kb on - strand at 213.965 kb on - strand at 213.965 kb on - strand at 213.965 kb on - strand at 213.978 kb on - strand at 214.151 kb on + strand, within Psyr_0200 at 214.151 kb on + strand, within Psyr_0200 at 214.151 kb on + strand, within Psyr_0200 at 214.152 kb on - strand, within Psyr_0200 at 214.167 kb on - strand, within Psyr_0200 at 214.187 kb on + strand, within Psyr_0200 at 214.187 kb on + strand, within Psyr_0200 at 214.187 kb on + strand, within Psyr_0200 at 214.187 kb on + strand, within Psyr_0200 at 214.188 kb on - strand, within Psyr_0200 at 214.188 kb on - strand, within Psyr_0200 at 214.188 kb on - strand, within Psyr_0200 at 214.244 kb on + strand, within Psyr_0200 at 214.418 kb on + strand, within Psyr_0200 at 214.418 kb on + strand, within Psyr_0200 at 214.418 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.490 kb on + strand, within Psyr_0200 at 214.491 kb on - strand, within Psyr_0200 at 214.491 kb on - strand, within Psyr_0200 at 214.491 kb on - strand, within Psyr_0200 at 214.491 kb on - strand, within Psyr_0200 at 214.508 kb on + strand, within Psyr_0200 at 214.603 kb on + strand, within Psyr_0200 at 214.652 kb on + strand, within Psyr_0200 at 214.655 kb on + strand, within Psyr_0200 at 214.656 kb on - strand, within Psyr_0200 at 215.006 kb on - strand, within Psyr_0200 at 215.006 kb on - strand, within Psyr_0200 at 215.006 kb on - strand, within Psyr_0200 at 215.083 kb on + strand, within Psyr_0200 at 215.084 kb on - strand, within Psyr_0200 at 215.205 kb on + strand, within Psyr_0200 at 215.206 kb on - strand, within Psyr_0200 at 215.206 kb on - strand, within Psyr_0200 at 215.291 kb on + strand at 215.291 kb on + strand at 215.291 kb on + strand at 215.292 kb on - strand at 215.292 kb on - strand at 215.292 kb on - strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.378 kb on + strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.379 kb on - strand at 215.406 kb on + strand at 215.406 kb on + strand at 215.406 kb on + strand at 215.407 kb on - strand at 215.407 kb on - strand at 215.407 kb on - strand at 215.407 kb on - strand at 215.408 kb on + strand at 215.409 kb on - strand at 215.451 kb on + strand at 215.451 kb on + strand at 215.451 kb on + strand at 215.451 kb on + strand at 215.452 kb on - strand at 215.452 kb on - strand at 215.452 kb on - strand at 215.452 kb on - strand at 215.452 kb on - strand at 215.452 kb on - strand at 216.019 kb on + strand, within Psyr_0201 at 216.019 kb on + strand, within Psyr_0201 at 216.019 kb on + strand, within Psyr_0201 at 216.019 kb on + strand, within Psyr_0201 at 216.019 kb on + strand, within Psyr_0201 at 216.020 kb on - strand, within Psyr_0201 at 216.020 kb on - strand, within Psyr_0201 at 216.020 kb on - strand, within Psyr_0201
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 213,010 + Psyr_0198 0.84 -0.3 213,010 + Psyr_0198 0.84 -0.4 213,059 + Psyr_0198 0.88 +0.0 213,060 - Psyr_0198 0.88 +0.6 213,060 - Psyr_0198 0.88 +0.7 213,172 + +0.3 213,172 + -0.7 213,172 + +0.1 213,172 + -0.0 213,172 + -0.9 213,172 + +0.7 213,173 - -1.0 213,173 - +1.1 213,173 - -1.2 213,173 - -1.8 213,173 - -0.5 213,185 + -0.3 213,232 + +0.6 213,232 + -0.0 213,232 + +0.3 213,232 + -0.2 213,233 - -1.3 213,233 - -0.1 213,233 - -0.4 213,233 - -0.0 213,235 + -0.6 213,235 + -1.7 213,236 - -2.2 213,236 - -2.2 213,281 - -0.1 213,343 + -0.6 213,343 + -1.2 213,344 - +1.6 213,344 - +0.3 213,352 + -1.0 213,352 + +0.6 213,352 + -0.4 213,353 - -0.4 213,353 - -2.6 213,353 - -0.0 213,422 + -0.4 213,423 - -0.6 213,423 - -1.7 213,423 - +0.1 213,423 - +1.1 213,423 - -1.5 213,472 - -0.1 213,696 - Psyr_0199 0.46 -1.5 213,722 + Psyr_0199 0.52 -0.5 213,722 + Psyr_0199 0.52 -1.2 213,847 - Psyr_0199 0.83 -0.4 213,959 + +0.9 213,959 + +0.5 213,959 + -0.5 213,959 + -0.9 213,959 + +1.1 213,959 + +2.6 213,959 + +0.9 213,960 - -1.4 213,960 - +1.1 213,960 - +1.0 213,960 - -1.2 213,960 - -1.2 213,960 - +0.9 213,960 - +2.1 213,964 + -0.2 213,964 + +1.0 213,964 + -0.1 213,964 + -1.0 213,964 + -2.7 213,964 + -0.6 213,965 - -0.3 213,965 - -0.5 213,965 - +2.0 213,965 - +1.9 213,978 - +0.8 214,151 + Psyr_0200 0.13 -1.0 214,151 + Psyr_0200 0.13 -3.9 214,151 + Psyr_0200 0.13 -0.2 214,152 - Psyr_0200 0.13 -0.8 214,167 - Psyr_0200 0.14 -2.1 214,187 + Psyr_0200 0.15 -1.1 214,187 + Psyr_0200 0.15 -2.2 214,187 + Psyr_0200 0.15 -0.5 214,187 + Psyr_0200 0.15 -0.7 214,188 - Psyr_0200 0.15 -2.6 214,188 - Psyr_0200 0.15 -1.1 214,188 - Psyr_0200 0.15 -0.1 214,244 + Psyr_0200 0.19 -2.2 214,418 + Psyr_0200 0.32 -1.2 214,418 + Psyr_0200 0.32 -1.1 214,418 + Psyr_0200 0.32 -1.7 214,490 + Psyr_0200 0.37 -1.6 214,490 + Psyr_0200 0.37 -2.7 214,490 + Psyr_0200 0.37 -3.6 214,490 + Psyr_0200 0.37 -2.0 214,490 + Psyr_0200 0.37 -2.3 214,490 + Psyr_0200 0.37 -1.8 214,491 - Psyr_0200 0.37 -2.7 214,491 - Psyr_0200 0.37 -2.9 214,491 - Psyr_0200 0.37 +0.1 214,491 - Psyr_0200 0.37 -1.1 214,508 + Psyr_0200 0.38 -4.2 214,603 + Psyr_0200 0.45 +0.8 214,652 + Psyr_0200 0.48 -1.0 214,655 + Psyr_0200 0.49 -2.9 214,656 - Psyr_0200 0.49 +0.7 215,006 - Psyr_0200 0.74 -2.2 215,006 - Psyr_0200 0.74 -2.2 215,006 - Psyr_0200 0.74 -2.1 215,083 + Psyr_0200 0.79 -2.7 215,084 - Psyr_0200 0.79 -4.2 215,205 + Psyr_0200 0.88 -2.4 215,206 - Psyr_0200 0.88 -3.2 215,206 - Psyr_0200 0.88 -2.2 215,291 + -0.8 215,291 + -2.4 215,291 + -4.3 215,292 - +0.7 215,292 - -1.0 215,292 - -1.0 215,378 + -0.1 215,378 + -3.0 215,378 + +1.8 215,378 + -2.8 215,378 + +1.5 215,378 + -0.6 215,378 + -1.7 215,378 + -3.4 215,378 + -2.6 215,378 + -3.2 215,378 + -1.7 215,378 + +0.2 215,378 + -1.2 215,378 + -0.8 215,378 + -3.9 215,378 + +0.5 215,379 - -1.6 215,379 - -1.1 215,379 - -0.9 215,379 - -0.6 215,379 - -3.0 215,379 - -0.8 215,379 - -1.1 215,379 - +0.3 215,379 - -0.3 215,379 - -3.0 215,379 - -2.3 215,379 - +0.4 215,379 - -0.4 215,379 - -0.7 215,406 + -0.8 215,406 + -2.8 215,406 + -3.2 215,407 - +0.3 215,407 - +1.3 215,407 - +1.7 215,407 - -0.6 215,408 + -0.0 215,409 - +0.1 215,451 + -2.6 215,451 + -2.6 215,451 + -0.9 215,451 + -2.5 215,452 - -1.3 215,452 - -0.7 215,452 - +0.9 215,452 - +1.7 215,452 - +1.0 215,452 - +0.9 216,019 + Psyr_0201 0.27 -2.0 216,019 + Psyr_0201 0.27 +0.5 216,019 + Psyr_0201 0.27 -0.2 216,019 + Psyr_0201 0.27 +1.2 216,019 + Psyr_0201 0.27 -0.6 216,020 - Psyr_0201 0.27 -0.4 216,020 - Psyr_0201 0.27 -1.3 216,020 - Psyr_0201 0.27 -1.0
Or see this region's nucleotide sequence