Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0166 and Psyr_0167 are separated by 5 nucleotides Psyr_0167 and Psyr_0168 are separated by 370 nucleotides
Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417
_0166
Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845
_0167
Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760
_0168
Position (kb)
181
182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 180.473 kb on - strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.722 kb on - strand, within Psyr_0166 at 180.748 kb on + strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 181.012 kb on + strand, within Psyr_0166 at 181.016 kb on + strand, within Psyr_0166 at 181.017 kb on - strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.092 kb on - strand, within Psyr_0166 at 181.151 kb on - strand, within Psyr_0166 at 181.184 kb on + strand, within Psyr_0166 at 181.274 kb on + strand, within Psyr_0166 at 181.286 kb on + strand, within Psyr_0166 at 181.287 kb on - strand at 181.288 kb on + strand at 181.289 kb on - strand at 181.328 kb on - strand at 181.387 kb on + strand at 181.611 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.696 kb on - strand, within Psyr_0167 at 181.798 kb on - strand, within Psyr_0167 at 181.843 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.848 kb on - strand at 181.848 kb on - strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.905 kb on - strand at 182.034 kb on - strand at 182.054 kb on - strand at 182.084 kb on + strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.104 kb on - strand at 182.128 kb on - strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.208 kb on - strand at 182.217 kb on + strand at 182.218 kb on - strand at 182.218 kb on - strand at 182.313 kb on + strand at 182.314 kb on - strand at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.532 kb on + strand, within Psyr_0168 at 182.533 kb on - strand, within Psyr_0168 at 182.533 kb on - strand, within Psyr_0168 at 182.744 kb on + strand, within Psyr_0168 at 182.835 kb on + strand, within Psyr_0168
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 180,473 - Psyr_0166 0.28 -0.2 180,529 + Psyr_0166 0.32 -1.4 180,529 + Psyr_0166 0.32 +0.2 180,533 + Psyr_0166 0.32 -0.0 180,533 + Psyr_0166 0.32 +1.7 180,534 - Psyr_0166 0.33 +0.3 180,534 - Psyr_0166 0.33 +1.2 180,534 - Psyr_0166 0.33 -0.8 180,534 - Psyr_0166 0.33 +1.7 180,534 - Psyr_0166 0.33 +0.6 180,534 - Psyr_0166 0.33 -0.7 180,534 - Psyr_0166 0.33 +0.8 180,722 - Psyr_0166 0.47 -0.6 180,748 + Psyr_0166 0.49 +1.7 180,847 - Psyr_0166 0.56 +1.0 180,847 - Psyr_0166 0.56 -0.7 180,995 + Psyr_0166 0.68 +1.5 180,995 + Psyr_0166 0.68 +0.4 180,995 + Psyr_0166 0.68 +0.1 180,995 + Psyr_0166 0.68 -0.4 180,996 - Psyr_0166 0.68 +0.2 180,996 - Psyr_0166 0.68 +1.4 180,997 + Psyr_0166 0.68 -1.4 180,997 + Psyr_0166 0.68 -0.7 180,997 + Psyr_0166 0.68 -0.9 180,997 + Psyr_0166 0.68 +0.5 181,012 + Psyr_0166 0.69 +0.4 181,016 + Psyr_0166 0.69 -0.4 181,017 - Psyr_0166 0.69 -0.8 181,042 + Psyr_0166 0.71 -1.4 181,042 + Psyr_0166 0.71 -1.3 181,092 - Psyr_0166 0.75 -1.1 181,151 - Psyr_0166 0.80 +0.9 181,184 + Psyr_0166 0.82 +2.4 181,274 + Psyr_0166 0.89 +0.8 181,286 + Psyr_0166 0.90 +0.5 181,287 - +0.5 181,288 + +1.3 181,289 - +0.2 181,328 - -0.0 181,387 + +0.1 181,611 + Psyr_0167 0.44 -2.3 181,693 + Psyr_0167 0.64 -2.3 181,693 + Psyr_0167 0.64 -2.7 181,693 + Psyr_0167 0.64 -2.4 181,693 + Psyr_0167 0.64 -2.9 181,694 - Psyr_0167 0.64 -1.3 181,694 - Psyr_0167 0.64 -1.5 181,696 - Psyr_0167 0.65 -1.0 181,798 - Psyr_0167 0.89 -1.4 181,843 + -0.6 181,847 + +1.6 181,847 + +0.2 181,847 + -1.1 181,848 - -0.1 181,848 - +1.5 181,904 + +0.1 181,904 + +0.0 181,904 + -0.2 181,905 - +0.0 182,034 - +0.5 182,054 - -0.2 182,084 + +0.4 182,086 + +0.2 182,087 - -1.8 182,087 - -0.5 182,104 - -0.6 182,128 - -0.3 182,207 + +0.3 182,207 + -2.3 182,208 - -0.3 182,217 + -1.6 182,218 - -0.2 182,218 - -0.2 182,313 + -1.4 182,314 - -1.6 182,532 + Psyr_0168 0.20 -1.6 182,532 + Psyr_0168 0.20 -2.7 182,532 + Psyr_0168 0.20 +0.9 182,532 + Psyr_0168 0.20 -0.1 182,532 + Psyr_0168 0.20 -2.0 182,533 - Psyr_0168 0.21 -2.7 182,533 - Psyr_0168 0.21 -0.8 182,744 + Psyr_0168 0.34 -2.6 182,835 + Psyr_0168 0.40 -2.1
Or see this region's nucleotide sequence