Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0144 and Psyr_0145 are separated by 47 nucleotides Psyr_0145 and Psyr_0146 are separated by 63 nucleotides Psyr_0146 and Psyr_0147 are separated by 284 nucleotides
Psyr_0144: Psyr_0144 - transcriptional regulator, AsnC family, at 157,030 to 157,518
_0144
Psyr_0145: Psyr_0145 - conserved hypothetical protein, at 157,566 to 158,240
_0145
Psyr_0146: Psyr_0146 - 4-aminobutyrate aminotransferase apoenzyme, at 158,304 to 159,608
_0146
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 157.338 kb on + strand, within Psyr_0144 at 157.338 kb on + strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.339 kb on - strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.352 kb on + strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.353 kb on - strand, within Psyr_0144 at 157.364 kb on + strand, within Psyr_0144 at 157.365 kb on - strand, within Psyr_0144 at 157.471 kb on + strand at 157.472 kb on - strand at 157.479 kb on + strand at 157.480 kb on - strand at 157.482 kb on - strand at 157.486 kb on - strand at 157.519 kb on + strand at 157.519 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.523 kb on + strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.524 kb on - strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.532 kb on + strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.533 kb on - strand at 157.551 kb on + strand at 157.552 kb on - strand at 157.552 kb on - strand at 157.558 kb on + strand at 157.559 kb on - strand at 157.620 kb on - strand at 157.625 kb on + strand at 157.625 kb on + strand at 157.864 kb on + strand, within Psyr_0145 at 157.864 kb on + strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.865 kb on - strand, within Psyr_0145 at 157.894 kb on + strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 157.895 kb on - strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.056 kb on + strand, within Psyr_0145 at 158.057 kb on - strand, within Psyr_0145 at 158.214 kb on - strand at 158.320 kb on - strand at 158.320 kb on - strand at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.647 kb on + strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.648 kb on - strand, within Psyr_0146 at 158.699 kb on - strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.127 kb on + strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.128 kb on - strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.167 kb on + strand, within Psyr_0146 at 159.341 kb on - strand, within Psyr_0146 at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.614 kb on + strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.615 kb on - strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.686 kb on + strand at 159.908 kb on + strand at 159.908 kb on + strand at 160.020 kb on - strand, within Psyr_0147 at 160.020 kb on - strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 157,338 + Psyr_0144 0.63 +0.1 157,338 + Psyr_0144 0.63 +0.3 157,339 - Psyr_0144 0.63 -1.5 157,339 - Psyr_0144 0.63 +0.1 157,339 - Psyr_0144 0.63 +0.5 157,339 - Psyr_0144 0.63 -0.5 157,352 + Psyr_0144 0.66 +0.3 157,352 + Psyr_0144 0.66 +0.4 157,352 + Psyr_0144 0.66 +0.0 157,352 + Psyr_0144 0.66 +0.3 157,353 - Psyr_0144 0.66 +2.4 157,353 - Psyr_0144 0.66 -0.5 157,353 - Psyr_0144 0.66 -0.1 157,353 - Psyr_0144 0.66 +0.8 157,364 + Psyr_0144 0.68 +0.1 157,365 - Psyr_0144 0.69 -1.5 157,471 + +0.4 157,472 - +1.8 157,479 + +0.7 157,480 - -1.5 157,482 - +0.7 157,486 - +0.6 157,519 + -0.3 157,519 + +1.6 157,523 + -0.2 157,523 + -1.1 157,523 + +1.4 157,523 + +1.9 157,523 + -0.9 157,523 + -1.3 157,524 - -1.1 157,524 - +0.5 157,524 - +1.1 157,524 - +0.6 157,524 - -1.3 157,532 + -0.3 157,532 + -0.5 157,532 + +0.3 157,532 + -0.3 157,532 + -0.1 157,532 + +0.0 157,532 + -1.0 157,532 + -0.7 157,532 + -0.0 157,532 + +0.6 157,532 + +0.4 157,532 + -1.2 157,532 + +0.8 157,532 + +0.1 157,532 + +0.0 157,532 + +1.0 157,532 + +1.2 157,532 + +0.3 157,532 + -0.3 157,532 + +2.0 157,532 + +1.8 157,532 + -1.5 157,532 + -0.6 157,532 + +4.1 157,532 + -0.6 157,533 - -0.1 157,533 - +0.5 157,533 - -0.2 157,533 - +0.1 157,533 - -0.7 157,533 - -0.9 157,533 - -0.6 157,533 - -1.0 157,533 - +0.0 157,533 - +3.0 157,533 - -2.1 157,533 - -0.8 157,533 - +0.1 157,533 - +0.2 157,533 - -0.7 157,533 - -0.2 157,551 + +0.4 157,552 - -0.8 157,552 - -0.5 157,558 + -0.3 157,559 - -1.4 157,620 - -0.6 157,625 + -0.7 157,625 + -0.3 157,864 + Psyr_0145 0.44 -2.0 157,864 + Psyr_0145 0.44 +0.1 157,865 - Psyr_0145 0.44 -0.0 157,865 - Psyr_0145 0.44 -1.1 157,865 - Psyr_0145 0.44 -1.5 157,894 + Psyr_0145 0.49 -0.6 157,895 - Psyr_0145 0.49 +0.7 157,895 - Psyr_0145 0.49 -0.3 158,056 + Psyr_0145 0.73 -1.4 158,056 + Psyr_0145 0.73 -0.4 158,056 + Psyr_0145 0.73 -0.0 158,056 + Psyr_0145 0.73 +0.5 158,057 - Psyr_0145 0.73 -0.3 158,214 - +1.0 158,320 - +0.0 158,320 - -0.1 158,647 + Psyr_0146 0.26 -0.3 158,647 + Psyr_0146 0.26 -0.7 158,647 + Psyr_0146 0.26 +1.1 158,647 + Psyr_0146 0.26 +1.1 158,647 + Psyr_0146 0.26 -1.2 158,647 + Psyr_0146 0.26 +0.2 158,647 + Psyr_0146 0.26 -0.1 158,647 + Psyr_0146 0.26 -1.6 158,647 + Psyr_0146 0.26 +1.3 158,648 - Psyr_0146 0.26 +0.2 158,648 - Psyr_0146 0.26 +1.3 158,648 - Psyr_0146 0.26 -0.3 158,648 - Psyr_0146 0.26 -2.1 158,648 - Psyr_0146 0.26 +0.4 158,648 - Psyr_0146 0.26 -0.7 158,648 - Psyr_0146 0.26 -0.4 158,648 - Psyr_0146 0.26 +0.5 158,648 - Psyr_0146 0.26 +0.0 158,648 - Psyr_0146 0.26 -1.1 158,648 - Psyr_0146 0.26 +1.0 158,648 - Psyr_0146 0.26 +0.0 158,648 - Psyr_0146 0.26 -0.0 158,648 - Psyr_0146 0.26 +0.0 158,648 - Psyr_0146 0.26 -1.3 158,648 - Psyr_0146 0.26 +0.4 158,699 - Psyr_0146 0.30 -1.1 159,127 + Psyr_0146 0.63 +0.9 159,127 + Psyr_0146 0.63 -1.6 159,128 - Psyr_0146 0.63 +0.7 159,128 - Psyr_0146 0.63 -0.2 159,167 + Psyr_0146 0.66 +0.2 159,167 + Psyr_0146 0.66 -1.2 159,341 - Psyr_0146 0.79 -0.9 159,614 + +0.6 159,614 + +0.9 159,614 + -1.9 159,614 + +0.4 159,614 + -1.8 159,614 + -1.6 159,614 + +1.5 159,614 + +0.0 159,614 + +0.1 159,614 + -0.8 159,614 + -0.6 159,614 + -0.8 159,614 + +1.3 159,614 + -0.5 159,614 + +1.0 159,614 + +1.4 159,615 - +1.8 159,615 - +2.4 159,615 - +0.3 159,615 - +0.5 159,615 - +0.1 159,615 - +0.7 159,615 - -1.0 159,615 - -0.7 159,615 - -0.4 159,615 - +0.2 159,615 - +0.0 159,615 - +0.3 159,615 - -1.4 159,615 - -0.2 159,686 + +2.5 159,686 + +2.1 159,686 + -1.1 159,686 + -0.2 159,908 + +1.0 159,908 + -0.6 160,020 - Psyr_0147 0.14 -2.4 160,020 - Psyr_0147 0.14 -1.0 160,333 + Psyr_0147 0.47 -1.0 160,333 + Psyr_0147 0.47 -1.6 160,334 - Psyr_0147 0.47 +0.2 160,334 - Psyr_0147 0.47 -1.0 160,415 + Psyr_0147 0.56 +0.9 160,416 - Psyr_0147 0.56 +0.9 160,416 - Psyr_0147 0.56 -0.0 160,416 - Psyr_0147 0.56 -0.6 160,556 - Psyr_0147 0.71 -0.7 160,556 - Psyr_0147 0.71 +1.8
Or see this region's nucleotide sequence