Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0120 and Psyr_0121 are separated by 81 nucleotides Psyr_0121 and Psyr_0122 are separated by 89 nucleotides
Psyr_0120: Psyr_0120 - Short-chain dehydrogenase/reductase SDR, at 130,166 to 130,936
_0120
Psyr_0121: Psyr_0121 - transcriptional regulator, TetR family, at 131,018 to 131,647
_0121
Psyr_0122: Psyr_0122 - NAD(P)H dehydrogenase (quinone), at 131,737 to 132,393
_0122
Position (kb)
131
132 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 130.058 kb on + strand at 130.059 kb on - strand at 130.110 kb on + strand at 130.110 kb on + strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.111 kb on - strand at 130.165 kb on + strand at 130.166 kb on - strand at 130.228 kb on + strand at 130.229 kb on - strand at 130.451 kb on - strand, within Psyr_0120 at 130.455 kb on + strand, within Psyr_0120 at 130.455 kb on + strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.456 kb on - strand, within Psyr_0120 at 130.459 kb on + strand, within Psyr_0120 at 130.459 kb on + strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.460 kb on - strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.575 kb on + strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.576 kb on - strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.670 kb on + strand, within Psyr_0120 at 130.671 kb on - strand, within Psyr_0120 at 130.747 kb on + strand, within Psyr_0120 at 130.748 kb on - strand, within Psyr_0120 at 130.748 kb on - strand, within Psyr_0120 at 130.748 kb on - strand, within Psyr_0120 at 130.761 kb on + strand, within Psyr_0120 at 130.761 kb on + strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.762 kb on - strand, within Psyr_0120 at 130.869 kb on + strand at 130.869 kb on + strand at 130.870 kb on - strand at 130.967 kb on + strand at 130.967 kb on + strand at 130.968 kb on - strand at 130.968 kb on - strand at 130.968 kb on - strand at 130.968 kb on - strand at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.147 kb on + strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.148 kb on - strand, within Psyr_0121 at 131.221 kb on + strand, within Psyr_0121 at 131.230 kb on + strand, within Psyr_0121 at 131.287 kb on + strand, within Psyr_0121 at 131.297 kb on + strand, within Psyr_0121 at 131.298 kb on - strand, within Psyr_0121 at 131.319 kb on - strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.331 kb on + strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.332 kb on - strand, within Psyr_0121 at 131.343 kb on - strand, within Psyr_0121 at 131.343 kb on - strand, within Psyr_0121 at 131.430 kb on + strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.431 kb on - strand, within Psyr_0121 at 131.459 kb on - strand, within Psyr_0121 at 131.635 kb on + strand at 131.651 kb on + strand at 131.652 kb on - strand at 131.652 kb on - strand at 131.655 kb on + strand at 131.656 kb on - strand at 131.661 kb on + strand at 131.665 kb on - strand at 131.691 kb on + strand at 131.694 kb on - strand at 131.735 kb on + strand at 131.736 kb on - strand at 131.814 kb on + strand, within Psyr_0122 at 131.815 kb on - strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.819 kb on + strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.820 kb on - strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.822 kb on + strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.823 kb on - strand, within Psyr_0122 at 131.844 kb on - strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.906 kb on + strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.907 kb on - strand, within Psyr_0122 at 131.960 kb on + strand, within Psyr_0122 at 131.960 kb on + strand, within Psyr_0122 at 131.961 kb on - strand, within Psyr_0122 at 131.961 kb on - strand, within Psyr_0122 at 131.961 kb on - strand, within Psyr_0122 at 131.990 kb on - strand, within Psyr_0122 at 131.998 kb on + strand, within Psyr_0122 at 131.998 kb on + strand, within Psyr_0122 at 132.090 kb on + strand, within Psyr_0122 at 132.090 kb on + strand, within Psyr_0122 at 132.091 kb on - strand, within Psyr_0122 at 132.116 kb on + strand, within Psyr_0122 at 132.116 kb on + strand, within Psyr_0122 at 132.116 kb on + strand, within Psyr_0122 at 132.116 kb on + strand, within Psyr_0122 at 132.117 kb on - strand, within Psyr_0122 at 132.117 kb on - strand, within Psyr_0122 at 132.117 kb on - strand, within Psyr_0122 at 132.131 kb on + strand, within Psyr_0122 at 132.131 kb on + strand, within Psyr_0122 at 132.132 kb on - strand, within Psyr_0122 at 132.132 kb on - strand, within Psyr_0122 at 132.154 kb on + strand, within Psyr_0122 at 132.154 kb on + strand, within Psyr_0122 at 132.154 kb on + strand, within Psyr_0122 at 132.155 kb on - strand, within Psyr_0122 at 132.155 kb on - strand, within Psyr_0122 at 132.155 kb on - strand, within Psyr_0122 at 132.155 kb on - strand, within Psyr_0122 at 132.180 kb on + strand, within Psyr_0122 at 132.181 kb on - strand, within Psyr_0122 at 132.204 kb on + strand, within Psyr_0122 at 132.204 kb on + strand, within Psyr_0122 at 132.205 kb on - strand, within Psyr_0122 at 132.205 kb on - strand, within Psyr_0122 at 132.205 kb on - strand, within Psyr_0122 at 132.205 kb on - strand, within Psyr_0122 at 132.214 kb on + strand, within Psyr_0122 at 132.234 kb on + strand, within Psyr_0122 at 132.234 kb on + strand, within Psyr_0122 at 132.234 kb on + strand, within Psyr_0122 at 132.234 kb on + strand, within Psyr_0122 at 132.234 kb on + strand, within Psyr_0122 at 132.235 kb on - strand, within Psyr_0122 at 132.235 kb on - strand, within Psyr_0122 at 132.235 kb on - strand, within Psyr_0122 at 132.235 kb on - strand, within Psyr_0122 at 132.245 kb on + strand, within Psyr_0122 at 132.246 kb on - strand, within Psyr_0122 at 132.246 kb on - strand, within Psyr_0122 at 132.312 kb on + strand, within Psyr_0122 at 132.313 kb on - strand, within Psyr_0122 at 132.345 kb on + strand at 132.359 kb on - strand at 132.359 kb on - strand at 132.391 kb on + strand at 132.391 kb on + strand at 132.392 kb on - strand at 132.392 kb on - strand at 132.392 kb on - strand at 132.397 kb on + strand at 132.397 kb on + strand at 132.433 kb on - strand at 132.457 kb on + strand at 132.458 kb on - strand at 132.458 kb on - strand at 132.458 kb on - strand at 132.478 kb on - strand at 132.478 kb on - strand at 132.578 kb on + strand at 132.589 kb on + strand at 132.590 kb on - strand at 132.590 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 130,058 + -0.3 130,059 - +0.4 130,110 + +0.8 130,110 + +0.4 130,111 - -1.1 130,111 - -0.7 130,111 - -0.9 130,111 - +1.9 130,111 - -1.0 130,165 + -0.0 130,166 - +0.9 130,228 + -0.8 130,229 - +1.0 130,451 - Psyr_0120 0.37 -0.2 130,455 + Psyr_0120 0.37 -0.2 130,455 + Psyr_0120 0.37 -1.1 130,456 - Psyr_0120 0.38 -1.2 130,456 - Psyr_0120 0.38 +0.4 130,456 - Psyr_0120 0.38 -0.8 130,456 - Psyr_0120 0.38 +0.1 130,459 + Psyr_0120 0.38 -0.4 130,459 + Psyr_0120 0.38 +1.0 130,460 - Psyr_0120 0.38 +1.8 130,460 - Psyr_0120 0.38 -1.8 130,460 - Psyr_0120 0.38 +0.2 130,460 - Psyr_0120 0.38 -0.7 130,460 - Psyr_0120 0.38 +0.5 130,460 - Psyr_0120 0.38 -0.1 130,575 + Psyr_0120 0.53 -0.8 130,575 + Psyr_0120 0.53 -0.2 130,575 + Psyr_0120 0.53 -0.1 130,575 + Psyr_0120 0.53 +0.0 130,575 + Psyr_0120 0.53 +1.2 130,576 - Psyr_0120 0.53 +1.0 130,576 - Psyr_0120 0.53 -1.5 130,670 + Psyr_0120 0.65 +0.2 130,670 + Psyr_0120 0.65 -1.4 130,670 + Psyr_0120 0.65 -0.3 130,670 + Psyr_0120 0.65 +1.9 130,671 - Psyr_0120 0.65 -0.7 130,747 + Psyr_0120 0.75 +2.3 130,748 - Psyr_0120 0.75 -0.7 130,748 - Psyr_0120 0.75 +0.1 130,748 - Psyr_0120 0.75 +0.9 130,761 + Psyr_0120 0.77 +0.1 130,761 + Psyr_0120 0.77 +1.0 130,762 - Psyr_0120 0.77 +0.6 130,762 - Psyr_0120 0.77 +0.6 130,762 - Psyr_0120 0.77 +0.2 130,762 - Psyr_0120 0.77 -0.7 130,762 - Psyr_0120 0.77 +0.3 130,762 - Psyr_0120 0.77 -0.2 130,762 - Psyr_0120 0.77 -2.8 130,762 - Psyr_0120 0.77 +1.1 130,762 - Psyr_0120 0.77 -0.0 130,762 - Psyr_0120 0.77 +0.5 130,869 + +0.5 130,869 + +0.1 130,870 - +1.0 130,967 + +0.5 130,967 + +0.3 130,968 - -0.9 130,968 - +1.3 130,968 - +3.0 130,968 - -0.6 131,147 + Psyr_0121 0.20 +0.1 131,147 + Psyr_0121 0.20 -2.5 131,147 + Psyr_0121 0.20 -2.1 131,147 + Psyr_0121 0.20 -1.4 131,148 - Psyr_0121 0.21 -0.2 131,148 - Psyr_0121 0.21 +1.3 131,148 - Psyr_0121 0.21 +0.7 131,148 - Psyr_0121 0.21 -1.5 131,148 - Psyr_0121 0.21 +1.1 131,148 - Psyr_0121 0.21 +0.4 131,148 - Psyr_0121 0.21 +0.2 131,221 + Psyr_0121 0.32 -1.4 131,230 + Psyr_0121 0.34 +0.3 131,287 + Psyr_0121 0.43 +0.2 131,297 + Psyr_0121 0.44 -0.6 131,298 - Psyr_0121 0.44 -0.9 131,319 - Psyr_0121 0.48 -0.5 131,331 + Psyr_0121 0.50 -0.5 131,331 + Psyr_0121 0.50 +0.1 131,331 + Psyr_0121 0.50 -0.4 131,331 + Psyr_0121 0.50 +1.1 131,331 + Psyr_0121 0.50 -1.1 131,331 + Psyr_0121 0.50 -2.4 131,331 + Psyr_0121 0.50 +0.1 131,331 + Psyr_0121 0.50 -1.8 131,331 + Psyr_0121 0.50 -1.7 131,331 + Psyr_0121 0.50 -0.6 131,331 + Psyr_0121 0.50 -0.6 131,332 - Psyr_0121 0.50 +0.3 131,332 - Psyr_0121 0.50 +0.0 131,332 - Psyr_0121 0.50 -0.3 131,332 - Psyr_0121 0.50 -0.7 131,332 - Psyr_0121 0.50 -0.4 131,332 - Psyr_0121 0.50 -2.4 131,332 - Psyr_0121 0.50 +1.7 131,332 - Psyr_0121 0.50 -0.6 131,343 - Psyr_0121 0.52 +1.2 131,343 - Psyr_0121 0.52 -1.0 131,430 + Psyr_0121 0.65 +1.5 131,431 - Psyr_0121 0.66 -0.5 131,431 - Psyr_0121 0.66 -0.6 131,431 - Psyr_0121 0.66 -2.1 131,459 - Psyr_0121 0.70 +0.7 131,635 + -0.4 131,651 + +2.2 131,652 - +1.7 131,652 - +0.1 131,655 + -1.5 131,656 - -2.4 131,661 + -2.0 131,665 - +1.2 131,691 + +0.6 131,694 - +0.1 131,735 + -1.4 131,736 - -1.7 131,814 + Psyr_0122 0.12 +1.5 131,815 - Psyr_0122 0.12 +1.8 131,819 + Psyr_0122 0.12 -0.7 131,819 + Psyr_0122 0.12 -1.8 131,819 + Psyr_0122 0.12 -0.9 131,819 + Psyr_0122 0.12 -1.0 131,819 + Psyr_0122 0.12 -0.9 131,819 + Psyr_0122 0.12 -1.7 131,820 - Psyr_0122 0.13 -0.8 131,820 - Psyr_0122 0.13 +0.7 131,820 - Psyr_0122 0.13 +0.7 131,822 + Psyr_0122 0.13 -0.4 131,822 + Psyr_0122 0.13 +1.0 131,822 + Psyr_0122 0.13 +0.0 131,822 + Psyr_0122 0.13 -1.9 131,822 + Psyr_0122 0.13 +0.7 131,822 + Psyr_0122 0.13 +0.2 131,823 - Psyr_0122 0.13 +0.2 131,823 - Psyr_0122 0.13 +1.0 131,823 - Psyr_0122 0.13 -0.8 131,823 - Psyr_0122 0.13 -1.8 131,844 - Psyr_0122 0.16 -0.6 131,906 + Psyr_0122 0.26 +0.1 131,906 + Psyr_0122 0.26 -0.8 131,906 + Psyr_0122 0.26 -0.1 131,906 + Psyr_0122 0.26 -0.5 131,906 + Psyr_0122 0.26 +0.7 131,906 + Psyr_0122 0.26 +0.3 131,906 + Psyr_0122 0.26 +1.2 131,906 + Psyr_0122 0.26 -2.3 131,906 + Psyr_0122 0.26 -0.1 131,906 + Psyr_0122 0.26 -0.2 131,907 - Psyr_0122 0.26 +0.5 131,907 - Psyr_0122 0.26 +0.1 131,907 - Psyr_0122 0.26 +0.9 131,907 - Psyr_0122 0.26 -1.1 131,907 - Psyr_0122 0.26 +1.6 131,907 - Psyr_0122 0.26 +0.6 131,907 - Psyr_0122 0.26 +1.6 131,907 - Psyr_0122 0.26 +0.8 131,907 - Psyr_0122 0.26 +0.9 131,907 - Psyr_0122 0.26 -0.1 131,960 + Psyr_0122 0.34 -1.2 131,960 + Psyr_0122 0.34 -1.2 131,961 - Psyr_0122 0.34 +0.4 131,961 - Psyr_0122 0.34 -0.9 131,961 - Psyr_0122 0.34 +2.1 131,990 - Psyr_0122 0.39 -0.0 131,998 + Psyr_0122 0.40 -0.8 131,998 + Psyr_0122 0.40 -1.4 132,090 + Psyr_0122 0.54 +2.8 132,090 + Psyr_0122 0.54 +1.7 132,091 - Psyr_0122 0.54 +1.0 132,116 + Psyr_0122 0.58 +0.6 132,116 + Psyr_0122 0.58 -0.9 132,116 + Psyr_0122 0.58 -1.4 132,116 + Psyr_0122 0.58 +0.3 132,117 - Psyr_0122 0.58 -0.1 132,117 - Psyr_0122 0.58 -0.1 132,117 - Psyr_0122 0.58 +0.2 132,131 + Psyr_0122 0.60 -1.2 132,131 + Psyr_0122 0.60 +1.7 132,132 - Psyr_0122 0.60 +1.4 132,132 - Psyr_0122 0.60 -0.2 132,154 + Psyr_0122 0.63 +0.8 132,154 + Psyr_0122 0.63 +1.1 132,154 + Psyr_0122 0.63 +1.1 132,155 - Psyr_0122 0.64 +1.2 132,155 - Psyr_0122 0.64 -1.5 132,155 - Psyr_0122 0.64 +0.8 132,155 - Psyr_0122 0.64 +1.3 132,180 + Psyr_0122 0.67 -0.7 132,181 - Psyr_0122 0.68 +0.8 132,204 + Psyr_0122 0.71 -0.1 132,204 + Psyr_0122 0.71 +0.5 132,205 - Psyr_0122 0.71 -0.3 132,205 - Psyr_0122 0.71 -0.6 132,205 - Psyr_0122 0.71 +0.2 132,205 - Psyr_0122 0.71 -0.2 132,214 + Psyr_0122 0.73 -0.9 132,234 + Psyr_0122 0.76 -0.0 132,234 + Psyr_0122 0.76 +0.0 132,234 + Psyr_0122 0.76 -0.7 132,234 + Psyr_0122 0.76 -1.4 132,234 + Psyr_0122 0.76 +0.9 132,235 - Psyr_0122 0.76 +0.4 132,235 - Psyr_0122 0.76 -0.5 132,235 - Psyr_0122 0.76 -0.7 132,235 - Psyr_0122 0.76 -3.6 132,245 + Psyr_0122 0.77 +0.1 132,246 - Psyr_0122 0.77 -1.5 132,246 - Psyr_0122 0.77 -2.1 132,312 + Psyr_0122 0.88 -0.1 132,313 - Psyr_0122 0.88 +0.3 132,345 + -0.2 132,359 - -0.1 132,359 - -0.7 132,391 + +0.3 132,391 + +0.2 132,392 - +1.0 132,392 - +0.4 132,392 - -0.1 132,397 + -0.6 132,397 + +0.2 132,433 - +1.9 132,457 + -1.1 132,458 - -0.5 132,458 - -0.8 132,458 - -0.3 132,478 - -0.4 132,478 - +0.8 132,578 + +1.7 132,589 + +1.4 132,590 - -0.2 132,590 - -0.5
Or see this region's nucleotide sequence