Experiment: library passed through aphids in food, 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0089 and Psyr_0090 are separated by 156 nucleotides Psyr_0090 and Psyr_0091 are separated by 179 nucleotides
Psyr_0089: Psyr_0089 - Response regulator receiver, at 96,943 to 98,331
_0089
Psyr_0090: Psyr_0090 - 4-aminobutyrate aminotransferase apoenzyme, at 98,488 to 99,768
_0090
Psyr_0091: Psyr_0091 - succinate semialdehyde dehydrogenase, at 99,948 to 101,390
_0091
Position (kb) 98
99
100 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 97.729 kb on + strand, within Psyr_0089 at 97.997 kb on + strand, within Psyr_0089 at 97.998 kb on - strand, within Psyr_0089 at 97.998 kb on - strand, within Psyr_0089 at 98.294 kb on - strand at 98.294 kb on - strand at 98.317 kb on + strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.318 kb on - strand at 98.382 kb on + strand at 98.399 kb on + strand at 98.399 kb on + strand at 98.400 kb on - strand at 98.400 kb on - strand at 98.400 kb on - strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.742 kb on - strand, within Psyr_0090 at 98.742 kb on - strand, within Psyr_0090 at 98.742 kb on - strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.873 kb on + strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.874 kb on - strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.912 kb on + strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.913 kb on - strand, within Psyr_0090 at 98.973 kb on + strand, within Psyr_0090 at 98.974 kb on - strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.122 kb on + strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.123 kb on - strand, within Psyr_0090 at 99.271 kb on - strand, within Psyr_0090 at 99.354 kb on - strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.509 kb on + strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.510 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.519 kb on - strand, within Psyr_0090 at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.770 kb on + strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.771 kb on - strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.840 kb on + strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.841 kb on - strand at 99.967 kb on + strand at 99.967 kb on + strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.968 kb on - strand at 99.983 kb on - strand at 99.997 kb on + strand at 99.997 kb on + strand at 99.997 kb on + strand at 100.094 kb on - strand, within Psyr_0091 at 100.141 kb on + strand, within Psyr_0091 at 100.147 kb on + strand, within Psyr_0091 at 100.147 kb on + strand, within Psyr_0091 at 100.148 kb on - strand, within Psyr_0091 at 100.266 kb on - strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.486 kb on + strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.487 kb on - strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.504 kb on + strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.505 kb on - strand, within Psyr_0091 at 100.547 kb on - strand, within Psyr_0091
Per-strain Table
Position Strand Gene LocusTag Fraction library passed through aphids in food, 72 hours remove 97,729 + Psyr_0089 0.57 -0.5 97,997 + Psyr_0089 0.76 +0.4 97,998 - Psyr_0089 0.76 -0.8 97,998 - Psyr_0089 0.76 +1.8 98,294 - -0.0 98,294 - -0.9 98,317 + +0.2 98,318 - +1.1 98,318 - -2.1 98,318 - -1.1 98,318 - -0.2 98,318 - +0.5 98,318 - -0.4 98,382 + -0.5 98,399 + -1.0 98,399 + -0.8 98,400 - +0.3 98,400 - -0.2 98,400 - -0.3 98,588 + -0.9 98,588 + -0.6 98,742 - Psyr_0090 0.20 -0.9 98,742 - Psyr_0090 0.20 +0.2 98,742 - Psyr_0090 0.20 +1.3 98,873 + Psyr_0090 0.30 +0.2 98,873 + Psyr_0090 0.30 -0.2 98,873 + Psyr_0090 0.30 +1.5 98,873 + Psyr_0090 0.30 -0.7 98,873 + Psyr_0090 0.30 -3.0 98,873 + Psyr_0090 0.30 +0.8 98,873 + Psyr_0090 0.30 +0.4 98,873 + Psyr_0090 0.30 +0.3 98,873 + Psyr_0090 0.30 +0.0 98,874 - Psyr_0090 0.30 -0.0 98,874 - Psyr_0090 0.30 +2.5 98,874 - Psyr_0090 0.30 +0.1 98,874 - Psyr_0090 0.30 +0.1 98,874 - Psyr_0090 0.30 -1.1 98,874 - Psyr_0090 0.30 -0.8 98,874 - Psyr_0090 0.30 -1.1 98,874 - Psyr_0090 0.30 -1.9 98,912 + Psyr_0090 0.33 +0.2 98,912 + Psyr_0090 0.33 -0.8 98,912 + Psyr_0090 0.33 -0.8 98,912 + Psyr_0090 0.33 +1.0 98,912 + Psyr_0090 0.33 -0.9 98,913 - Psyr_0090 0.33 +0.1 98,913 - Psyr_0090 0.33 +1.4 98,913 - Psyr_0090 0.33 +1.1 98,913 - Psyr_0090 0.33 -1.9 98,913 - Psyr_0090 0.33 +0.1 98,973 + Psyr_0090 0.38 +2.5 98,974 - Psyr_0090 0.38 -2.0 99,122 + Psyr_0090 0.49 -0.8 99,122 + Psyr_0090 0.49 +0.7 99,122 + Psyr_0090 0.49 -2.7 99,122 + Psyr_0090 0.49 -0.7 99,122 + Psyr_0090 0.49 +0.0 99,122 + Psyr_0090 0.49 +1.3 99,122 + Psyr_0090 0.49 -0.4 99,122 + Psyr_0090 0.49 -2.4 99,123 - Psyr_0090 0.50 +0.8 99,123 - Psyr_0090 0.50 -1.5 99,123 - Psyr_0090 0.50 +0.2 99,123 - Psyr_0090 0.50 -0.6 99,123 - Psyr_0090 0.50 -1.1 99,123 - Psyr_0090 0.50 +4.0 99,123 - Psyr_0090 0.50 +2.5 99,123 - Psyr_0090 0.50 +2.4 99,123 - Psyr_0090 0.50 +3.6 99,123 - Psyr_0090 0.50 +0.2 99,123 - Psyr_0090 0.50 +0.6 99,123 - Psyr_0090 0.50 -0.8 99,123 - Psyr_0090 0.50 +0.7 99,123 - Psyr_0090 0.50 -1.3 99,271 - Psyr_0090 0.61 +0.5 99,354 - Psyr_0090 0.68 +0.5 99,509 + Psyr_0090 0.80 +1.1 99,509 + Psyr_0090 0.80 -0.7 99,510 - Psyr_0090 0.80 +0.3 99,510 - Psyr_0090 0.80 -0.6 99,510 - Psyr_0090 0.80 -0.1 99,510 - Psyr_0090 0.80 +0.2 99,510 - Psyr_0090 0.80 +1.5 99,510 - Psyr_0090 0.80 +0.4 99,510 - Psyr_0090 0.80 +0.2 99,519 - Psyr_0090 0.80 -0.5 99,519 - Psyr_0090 0.80 +1.5 99,519 - Psyr_0090 0.80 -0.8 99,770 + +2.2 99,770 + +0.4 99,770 + +0.1 99,770 + -1.1 99,770 + +0.5 99,770 + -0.1 99,770 + +0.0 99,770 + -1.1 99,770 + +0.4 99,770 + -0.1 99,770 + -1.3 99,770 + -0.6 99,770 + +1.1 99,770 + +0.7 99,770 + +0.8 99,770 + +0.8 99,770 + -0.2 99,770 + -2.0 99,770 + -1.0 99,770 + +1.3 99,771 - +0.3 99,771 - +1.0 99,771 - +1.2 99,771 - +2.5 99,771 - -1.0 99,771 - +0.3 99,771 - -0.9 99,771 - -1.5 99,771 - -0.1 99,771 - +1.3 99,771 - -1.4 99,771 - -0.2 99,771 - -2.9 99,771 - +0.5 99,771 - +0.1 99,771 - -0.1 99,840 + +0.6 99,840 + +0.2 99,840 + +2.5 99,840 + +0.0 99,841 - -1.7 99,841 - +0.9 99,841 - +0.4 99,841 - +0.2 99,841 - +0.1 99,967 + -0.2 99,967 + +3.2 99,968 - +1.1 99,968 - +1.3 99,968 - -1.0 99,968 - +0.7 99,968 - +0.2 99,968 - +2.2 99,983 - +1.5 99,997 + -0.3 99,997 + +0.1 99,997 + -1.6 100,094 - Psyr_0091 0.10 -1.0 100,141 + Psyr_0091 0.13 -1.8 100,147 + Psyr_0091 0.14 -0.1 100,147 + Psyr_0091 0.14 +0.0 100,148 - Psyr_0091 0.14 +0.8 100,266 - Psyr_0091 0.22 -0.5 100,486 + Psyr_0091 0.37 -0.3 100,486 + Psyr_0091 0.37 -1.5 100,487 - Psyr_0091 0.37 +0.2 100,487 - Psyr_0091 0.37 -0.7 100,487 - Psyr_0091 0.37 +1.9 100,504 + Psyr_0091 0.39 -0.8 100,504 + Psyr_0091 0.39 -0.0 100,504 + Psyr_0091 0.39 +0.6 100,504 + Psyr_0091 0.39 +0.0 100,505 - Psyr_0091 0.39 +0.8 100,505 - Psyr_0091 0.39 -0.4 100,505 - Psyr_0091 0.39 -1.1 100,505 - Psyr_0091 0.39 -1.7 100,505 - Psyr_0091 0.39 -0.1 100,505 - Psyr_0091 0.39 +1.8 100,505 - Psyr_0091 0.39 +0.4 100,505 - Psyr_0091 0.39 +0.0 100,505 - Psyr_0091 0.39 +1.7 100,547 - Psyr_0091 0.42 +0.6
Or see this region's nucleotide sequence