Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotides Pf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotides Pf6N2E2_670 and Pf6N2E2_671 are separated by 214 nucleotides
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945
_669
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Position (kb)
775
776
777 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.746 kb on - strand, within Pf6N2E2_669 at 774.782 kb on + strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.858 kb on - strand, within Pf6N2E2_669 at 774.938 kb on + strand at 774.938 kb on + strand at 774.939 kb on - strand at 775.072 kb on + strand at 775.084 kb on + strand at 775.147 kb on + strand at 775.147 kb on + strand at 775.148 kb on - strand at 775.148 kb on - strand at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.417 kb on - strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.588 kb on + strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 776.032 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.042 kb on + strand at 776.043 kb on - strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.058 kb on - strand at 776.058 kb on - strand at 776.061 kb on + strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.117 kb on + strand at 776.117 kb on + strand at 776.141 kb on + strand at 776.328 kb on - strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.739 kb on - strand, within Pf6N2E2_671
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 774,147 + Pf6N2E2_668 0.84 +0.6 774,148 - Pf6N2E2_668 0.84 -0.2 774,148 - Pf6N2E2_668 0.84 -0.8 774,148 - Pf6N2E2_668 0.84 +0.3 774,466 - Pf6N2E2_669 0.14 +1.8 774,466 - Pf6N2E2_669 0.14 -1.1 774,746 - Pf6N2E2_669 0.64 +2.9 774,782 + Pf6N2E2_669 0.70 +0.7 774,783 - Pf6N2E2_669 0.71 -0.1 774,783 - Pf6N2E2_669 0.71 -1.0 774,858 - Pf6N2E2_669 0.84 +0.5 774,938 + -3.5 774,938 + +0.2 774,939 - +1.1 775,072 + +0.6 775,084 + +1.0 775,147 + -1.5 775,147 + -2.9 775,148 - -0.4 775,148 - +0.0 775,288 + Pf6N2E2_670 0.22 +0.2 775,288 + +1.4 775,289 - Pf6N2E2_670 0.22 +0.1 775,289 - Pf6N2E2_670 0.22 -0.2 775,289 - Pf6N2E2_670 0.22 +0.3 775,416 + Pf6N2E2_670 0.36 -0.7 775,416 + Pf6N2E2_670 0.36 -0.2 775,416 + Pf6N2E2_670 0.36 -1.1 775,417 - Pf6N2E2_670 0.36 -0.2 775,570 + Pf6N2E2_670 0.53 +0.6 775,570 + Pf6N2E2_670 0.53 +1.3 775,571 - Pf6N2E2_670 0.53 +0.7 775,571 - Pf6N2E2_670 0.53 -1.1 775,588 + Pf6N2E2_670 0.55 +1.2 775,589 - Pf6N2E2_670 0.55 -1.8 775,589 - Pf6N2E2_670 0.55 -0.4 775,589 - Pf6N2E2_670 0.55 +2.0 775,663 + Pf6N2E2_670 0.63 +0.7 775,663 + Pf6N2E2_670 0.63 +0.2 776,032 + -1.0 776,034 + +0.1 776,034 + -0.7 776,034 + -1.7 776,034 + -0.5 776,034 + -0.4 776,034 + -0.4 776,034 + +0.9 776,035 - +0.2 776,035 - +1.4 776,035 - -0.8 776,035 - +0.0 776,042 + -0.4 776,043 - +1.2 776,057 + -3.4 776,057 + +0.4 776,057 + +0.5 776,057 + -1.3 776,057 + -0.4 776,058 - +0.7 776,058 - -1.8 776,061 + +0.0 776,062 - +1.0 776,062 - -2.5 776,062 - +0.6 776,117 + -0.2 776,117 + +0.5 776,141 + +0.2 776,328 - Pf6N2E2_671 0.11 +1.1 776,406 + Pf6N2E2_671 0.19 +0.5 776,406 + Pf6N2E2_671 0.19 -0.4 776,454 + Pf6N2E2_671 0.23 -0.7 776,454 + Pf6N2E2_671 0.23 +0.2 776,739 - Pf6N2E2_671 0.51 +0.6
Or see this region's nucleotide sequence