Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_667 and Pf6N2E2_668 are separated by 55 nucleotides Pf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotides Pf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotides
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320
_668
Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945
_669
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Position (kb) 773
774
775 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.388 kb on + strand, within Pf6N2E2_668 at 773.389 kb on - strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.568 kb on + strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.569 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.572 kb on - strand, within Pf6N2E2_668 at 773.590 kb on - strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 773.676 kb on + strand, within Pf6N2E2_668 at 774.147 kb on + strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.148 kb on - strand, within Pf6N2E2_668 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.466 kb on - strand, within Pf6N2E2_669 at 774.746 kb on - strand, within Pf6N2E2_669 at 774.782 kb on + strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.783 kb on - strand, within Pf6N2E2_669 at 774.858 kb on - strand, within Pf6N2E2_669 at 774.938 kb on + strand at 774.938 kb on + strand at 774.939 kb on - strand at 775.072 kb on + strand at 775.084 kb on + strand at 775.147 kb on + strand at 775.147 kb on + strand at 775.148 kb on - strand at 775.148 kb on - strand at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 772,782 - Pf6N2E2_667 0.65 -0.2 772,895 + Pf6N2E2_667 0.75 +0.9 772,895 + Pf6N2E2_667 0.75 +1.9 772,896 - Pf6N2E2_667 0.75 +0.3 772,896 - Pf6N2E2_667 0.75 -0.7 772,948 - Pf6N2E2_667 0.80 +0.6 772,989 + Pf6N2E2_667 0.84 +0.1 772,990 - Pf6N2E2_667 0.84 -0.8 773,388 + Pf6N2E2_668 0.15 +0.9 773,388 + Pf6N2E2_668 0.15 +0.5 773,389 - Pf6N2E2_668 0.15 +1.4 773,568 + Pf6N2E2_668 0.31 +0.1 773,568 + Pf6N2E2_668 0.31 -0.1 773,569 - Pf6N2E2_668 0.31 -2.7 773,569 - Pf6N2E2_668 0.31 +0.4 773,572 - Pf6N2E2_668 0.32 +0.8 773,572 - Pf6N2E2_668 0.32 +0.8 773,572 - Pf6N2E2_668 0.32 +0.6 773,572 - Pf6N2E2_668 0.32 +1.2 773,590 - Pf6N2E2_668 0.33 +0.7 773,676 + Pf6N2E2_668 0.41 -0.1 773,676 + Pf6N2E2_668 0.41 +0.4 774,147 + Pf6N2E2_668 0.84 +0.6 774,148 - Pf6N2E2_668 0.84 -0.2 774,148 - Pf6N2E2_668 0.84 -0.8 774,148 - Pf6N2E2_668 0.84 +0.3 774,466 - Pf6N2E2_669 0.14 +1.8 774,466 - Pf6N2E2_669 0.14 -1.1 774,746 - Pf6N2E2_669 0.64 +2.9 774,782 + Pf6N2E2_669 0.70 +0.7 774,783 - Pf6N2E2_669 0.71 -0.1 774,783 - Pf6N2E2_669 0.71 -1.0 774,858 - Pf6N2E2_669 0.84 +0.5 774,938 + -3.5 774,938 + +0.2 774,939 - +1.1 775,072 + +0.6 775,084 + +1.0 775,147 + -1.5 775,147 + -2.9 775,148 - -0.4 775,148 - +0.0 775,288 + Pf6N2E2_670 0.22 +0.2 775,288 + +1.4 775,289 - Pf6N2E2_670 0.22 +0.1 775,289 - Pf6N2E2_670 0.22 -0.2 775,289 - Pf6N2E2_670 0.22 +0.3
Or see this region's nucleotide sequence